C57BL/6JCya-Zic3em1flox/Cya
Common Name
Zic3-flox
Product ID
S-CKO-07112
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-22773-Zic3-B6J-VA
Status
When using this mouse strain in a publication, please cite “Zic3-flox Mouse (Catalog S-CKO-07112) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Zic3-flox
Strain ID
CKOCMP-22773-Zic3-B6J-VA
Gene Name
Product ID
S-CKO-07112
Gene Alias
Bn, Ka
Background
C57BL/6JCya
Gene Full Name
zinc finger protein of the cerebellum 3
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr X (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000088627
NCBI Transcript ID
NM_009575
Target Region
Exon 1
Size of Effective Region
~2.3 kb
Overview of Gene Research
Zic3, a C2H2 zinc finger transcription factor, is crucial for early vertebrate embryo patterning. It has putative roles in multiple developmental signalling pathways involved in establishing the left-right axis. Zic3 is also important for midline neural patterning, and its function is linked to maintaining pluripotency of embryonic stem cells [1,2,3,7].
In Zic3 null mice, which recapitulate the human heterotaxy phenotype, there are cilia positioning defects at the embryonic node due to impaired planar cell polarity (PCP), leading to abnormal left-right signalling, heart defects, and neural tube defects. Zic3 interacts with PCP membrane protein Vangl2 and effector genes Rac1 and Daam1, and its absence disrupts the expression of PCP components and reduces phosphorylation of DVL2 during left-right axis determination. Conditional deletion in mice shows Zic3 is required in the primitive streak and migrating mesoderm for proper left-right patterning and cardiac development [4,5]. In Xenopus embryos, overexpression of Zic3 on the right side altered heart and gut looping, and its expression in the mesoderm is induced by activin or Vg1, suggesting it participates in left-sided signalling upstream of Xnr1 and Pitx2 [6].
In conclusion, Zic3 is essential for left-right axis formation, midline neural patterning, and maintaining pluripotency. Studies using Zic3 knockout and conditional knockout mouse models, as well as Xenopus models, have revealed its role in these processes and in diseases such as heterotaxy, congenital heart disease, and neural tube defects. These models have provided key insights into the molecular mechanisms underlying Zic3-related biological functions and disease conditions [1,4,5,6].
References：
1. Herman, G E, El-Hodiri, H M. . The role of ZIC3 in vertebrate development. In Cytogenetic and genome research, 99, 229-35. doi:. https://pubmed.ncbi.nlm.nih.gov/12900569/
2. Hossain, Ishtiaque, Priam, Pierre, Reynoso, Sofia C, Lessard, Julie A, Pastor, William A. 2024. ZIC2 and ZIC3 promote SWI/SNF recruitment to safeguard progression towards human primed pluripotency. In Nature communications, 15, 8539. doi:10.1038/s41467-024-52431-1. https://pubmed.ncbi.nlm.nih.gov/39358345/
3. Yang, Shen-Hsi, Andrabi, Munazah, Biss, Rebecca, Iqbal, Mudassar, Sharrocks, Andrew D. . ZIC3 Controls the Transition from Naive to Primed Pluripotency. In Cell reports, 27, 3215-3227.e6. doi:10.1016/j.celrep.2019.05.026. https://pubmed.ncbi.nlm.nih.gov/31189106/
4. Bellchambers, Helen M, Ware, Stephanie M. . Loss of Zic3 impairs planar cell polarity leading to abnormal left-right signaling, heart defects and neural tube defects. In Human molecular genetics, 30, 2402-2415. doi:10.1093/hmg/ddab195. https://pubmed.ncbi.nlm.nih.gov/34274973/
5. Sutherland, Mardi J, Wang, Shuyun, Quinn, Malgorzata E, Haaning, Allison, Ware, Stephanie M. 2013. Zic3 is required in the migrating primitive streak for node morphogenesis and left-right patterning. In Human molecular genetics, 22, 1913-23. doi:10.1093/hmg/ddt001. https://pubmed.ncbi.nlm.nih.gov/23303524/
6. Kitaguchi, T, Nagai, T, Nakata, K, Aruga, J, Mikoshiba, K. . Zic3 is involved in the left-right specification of the Xenopus embryo. In Development (Cambridge, England), 127, 4787-95. doi:. https://pubmed.ncbi.nlm.nih.gov/11044394/
7. Declercq, Jeroen, Sheshadri, Preethi, Verfaillie, Catherine M, Kumar, Anujith. 2013. Zic3 enhances the generation of mouse induced pluripotent stem cells. In Stem cells and development, 22, 2017-25. doi:10.1089/scd.2012.0651. https://pubmed.ncbi.nlm.nih.gov/23421367/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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