C57BL/6NCya-Niban2em1flox/Cya
Common Name:
Niban2-flox
Product ID:
S-CKO-07116
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Niban2-flox
Strain ID
CKOCMP-227737-Niban2-B6N-VA
Gene Name
Product ID
S-CKO-07116
Gene Alias
9130404D14Rik; Fam129b; Meg-3
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Niban2em1flox/Cya mice (Catalog S-CKO-07116) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028135
NCBI RefSeq
NM_146119
Target Region
Exon 4
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Niban2, with currently no widely-known common aliases, has been found to be tightly associated with multiple biological processes. It is involved in pathways related to bone formation, cell migration, and may play a role in immune-related functions. In bone, it promotes osteoblast differentiation, which is crucial for maintaining normal bone structure and preventing osteoporosis [1].
Conditional Niban2 knockout in osteoblasts led to bone loss and insufficient mineralization, demonstrating its key role in bone formation. Mechanistically, Niban2 interacts with the HNRNPU-cored spliceosome complex, regulating the alternative splicing of RUNX2. This results in an increase in functional RUNX2 (nuclear localization sequence complete) and a decrease in dysfunctional RUNX2 (exon 6 exclusive), ultimately reinforcing osteoblast differentiation.
Additionally, in bovine cloned placentation, NIBAN2 was upregulated, and in human endometrial sEV-mediated trophoblast invasion, NIBAN2 was among the upregulated proteins related to embryo implantation. Also, in FLT3-mutated AML, NIBAN2 was identified as a candidate biomarker associated with FLT3 inhibitor sensitivity [1,2,3,4].
In conclusion, Niban2 plays essential roles in bone formation, potentially through its regulation of RUNX2 alternative splicing. Its involvement in processes like cell migration during placentation and as a biomarker in AML indicates its broad-reaching biological significance. The conditional knockout mouse model in osteoblasts has been particularly valuable in revealing its role in bone-related diseases such as osteoporosis.
References：
1. Zhang, Sheng, Yang, Zhiqiang, Xie, Yuanlong, Cai, Lin, Wei, Renxiong. 2025. Identification of NIBAN2-Regulated RUNX2 Alternative Splicing Presents Novel Strategies for Antagonizing Osteoporosis. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 12, e2416536. doi:10.1002/advs.202416536. https://pubmed.ncbi.nlm.nih.gov/40051391/
2. Barreto, Rodrigo da Silva Nunes, Matias, Gustavo de Sá Schiavo, Nishiyama-Jr, Milton Yutaka, Carreira, Ana Claudia Oliveira, Miglino, Maria Angelica. 2022. ECM proteins involved in cell migration and vessel formation compromise bovine cloned placentation. In Theriogenology, 188, 156-162. doi:10.1016/j.theriogenology.2022.04.003. https://pubmed.ncbi.nlm.nih.gov/35689945/
3. Fatmous, Monique, Rai, Alin, Poh, Qi Hui, Salamonsen, Lois A, Greening, David W. 2022. Endometrial small extracellular vesicles regulate human trophectodermal cell invasion by reprogramming the phosphoproteome landscape. In Frontiers in cell and developmental biology, 10, 1078096. doi:10.3389/fcell.2022.1078096. https://pubmed.ncbi.nlm.nih.gov/36619864/
4. Niu, Mingming, Wang, Ning, Yang, Dandan, Wang, Hong, Shao, Xianfeng. 2025. Multi-omics integration reveals immune hallmarks and biomarkers associated with FLT3 inhibitor sensitivity in FLT3-mutated AML. In Blood science (Baltimore, Md.), 7, e00227. doi:10.1097/BS9.0000000000000227. https://pubmed.ncbi.nlm.nih.gov/40115132/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen