C57BL/6JCya-Gcaem1flox/Cya
Common Name:
Gca-flox
Product ID:
S-CKO-07144
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gca-flox
Strain ID
CKOCMP-227960-Gca-B6J-VA
Gene Name
Product ID
S-CKO-07144
Gene Alias
5133401E04Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gcaem1flox/Cya mice (Catalog S-CKO-07144) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028257
NCBI RefSeq
NM_145523
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Gca, also known as grancalcin, is a protein whose levels in circulation are associated with cognitive function. Bone marrow-derived GCA+ immune cells, which secrete Gca, play a role in the pathogenesis of Alzheimer's disease (AD). These cells preferentially invade the hippocampus and cortex of AD mouse models in a C-C Motif Chemokine Receptor 10 (CCR10)-dependent manner [1].
In AD research, transplanting GCA+ immune cells or injecting recombinant GCA protein intensifies amyloid plaque load and aggravates cognitive and memory impairments. Conversely, genetic ablation of GCA in the hematopoietic compartment improves cognitive and memory function. Mechanistically, GCA competitively binds to the low-density lipoprotein receptor-related protein 1 (LRP1) in microglia, inhibiting phagocytosis and clearance of Aβ and potentiating neuropathological changes. GCA-neutralizing antibody treatment rejuvenates cognitive and memory function and constrains AD progression [1].
In conclusion, Gca-related research, especially through genetic ablation models, reveals that Gca+ immune cells play a pathological role in cognitive and memory decline in AD. Understanding Gca's function provides potential targets for innovative therapeutic strategies in AD.
References：
1. Zhou, Rui, Wang, Liwen, Chen, Linyun, Huang, Yan, Zhou, Haiyan. 2023. Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2303402. doi:10.1002/advs.202303402. https://pubmed.ncbi.nlm.nih.gov/37949676/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen