C57BL/6JCya-Pdk1em1flox/Cya
Common Name:
Pdk1-flox
Product ID:
S-CKO-07150
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pdk1-flox
Strain ID
CKOCMP-228026-Pdk1-B6J-VA
Gene Name
Product ID
S-CKO-07150
Gene Alias
B830012B01; D530020C15Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pdk1em1flox/Cya mice (Catalog S-CKO-07150) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000006669
NCBI RefSeq
NM_172665
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Pdk1, also known as 3-phosphoinositide-dependent protein kinase 1, is a master kinase regulating AGC kinase family members. It plays a crucial role in the PI3K/Akt, Ras/MAPK and Myc signaling pathways [1,2]. It is involved in multiple biological processes such as cell growth, metastasis, invasion, apoptosis and survival, making it of great biological importance [1]. Genetic mouse models are valuable tools for studying Pdk1.
In a genetic mouse model, Smurf1 deficiency dramatically reduced colorectal cancer tumorigenesis, unveiling the role of Smurf1-mediated PDK1 neddylation in PI3K-Akt signaling and tumorigenesis [3]. In breast cancer, PDK1 knockdown reduced the proliferation, migration, and tumorigenicity of breast cancer cells and inhibited the HIF-1α pathway [4]. Also, downregulation of PDK1 levels inhibited migration and experimental metastasis of human breast cancer cells [5].
In conclusion, Pdk1 is essential in regulating cell behaviors and signaling pathways. Mouse models have revealed its significant roles in cancer development, including colorectal and breast cancer. Targeting Pdk1 may be a potential strategy for cancer therapy.
References：
1. Zheng, Nana, Wei, Jiaqi, Wu, Depei, Xu, Yang, Guo, Jianping. 2023. Master kinase PDK1 in tumorigenesis. In Biochimica et biophysica acta. Reviews on cancer, 1878, 188971. doi:10.1016/j.bbcan.2023.188971. https://pubmed.ncbi.nlm.nih.gov/37640147/
2. Gagliardi, Paolo Armando, Puliafito, Alberto, Primo, Luca. 2017. PDK1: At the crossroad of cancer signaling pathways. In Seminars in cancer biology, 48, 27-35. doi:10.1016/j.semcancer.2017.04.014. https://pubmed.ncbi.nlm.nih.gov/28473254/
3. Peng, Zhiqiang, Fang, Wei, Wu, Bo, Cui, Chun-Ping, Zhang, Lingqiang. 2024. Targeting Smurf1 to block PDK1-Akt signaling in KRAS-mutated colorectal cancer. In Nature chemical biology, 21, 59-70. doi:10.1038/s41589-024-01683-5. https://pubmed.ncbi.nlm.nih.gov/39039255/
4. Wei, Yu, Zhang, Dian, Shi, He, Guo, Bianqin, Chen, Tingmei. 2023. PDK1 promotes breast cancer progression by enhancing the stability and transcriptional activity of HIF-1α. In Genes & diseases, 11, 101041. doi:10.1016/j.gendis.2023.06.013. https://pubmed.ncbi.nlm.nih.gov/38560503/
5. Raimondi, C, Falasca, M. . Targeting PDK1 in cancer. In Current medicinal chemistry, 18, 2763-9. doi:. https://pubmed.ncbi.nlm.nih.gov/21568903/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen