C57BL/6JCya-Kat14em1flox/Cya
Common Name:
Kat14-flox
Product ID:
S-CKO-07183
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Kat14-flox
Strain ID
CKOCMP-228714-Kat14-B6J-VA
Gene Name
Product ID
S-CKO-07183
Gene Alias
2510008M08Rik; ATAC2; Csrp2bp; D2Ertd473e; D2Wsu131e; E430020F17
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kat14em1flox/Cya mice (Catalog S-CKO-07183) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028911
NCBI RefSeq
NM_181417
Target Region
Exon 6~7
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Kat14, also known as Atac2, is a histone acetyltransferase. It is part of the hATAC complex and has histone acetyl transferase activity, preferentially acetylating histone H4 [2,4]. It is involved in various biological processes related to chromatin-remodeling, gene expression regulation, and may play a role in the epigenetic machinery [2,4,5].
In ovarian endometrioma, Kat14 was upregulated in ectopic lesions and predominantly expressed in activated fibroblasts. In vitro studies in endometrial stromal cells showed that Kat14 overexpression promoted a TGF-β-induced profibrotic response, while silencing had adverse effects. In an endometriosis mouse model, Kat14 knockdown ameliorated fibrosis in ectopic lesions. Mechanistically, Kat14 directly interacts with serum response factor (SRF) to promote the expression of α-smooth muscle actin (α-SMA) by increasing histone H4 acetylation at promoter regions, which is necessary for TGF-β-induced ECM production and myofibroblast differentiation [1]. In the pea aphid, silencing of Kat14 promoted survival or increased the number of offspring, suggesting its role in life-history traits [3].
In conclusion, Kat14 is an important histone acetyltransferase involved in chromatin-remodeling and gene expression. Its role in promoting fibrosis in ovarian endometrioma through interaction with SRF has been revealed by in vitro and in vivo studies, especially the use of gene knockdown in a mouse model. Also, its impact on the life-history traits of the pea aphid indicates its broad-ranging biological functions.
References:
1. Gong, Yi, Liu, Mian, Zhang, Qianqian, Ma, Yanlin, Quan, Song. 2024. Lysine acetyltransferase 14 mediates TGF-β-induced fibrosis in ovarian endometrioma via co-operation with serum response factor. In Journal of translational medicine, 22, 561. doi:10.1186/s12967-024-05243-2. https://pubmed.ncbi.nlm.nih.gov/38867256/
2. Viita, Tiina, Kyheröinen, Salla, Prajapati, Bina, Varjosalo, Markku, Vartiainen, Maria K. 2019. Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing. In Journal of cell science, 132, . doi:10.1242/jcs.226852. https://pubmed.ncbi.nlm.nih.gov/30890647/
3. Kirfel, Phillipp, Skaljac, Marisa, Grotmann, Jens, Michaelis, Katja, Vilcinskas, Andreas. 2019. Inhibition of histone acetylation and deacetylation enzymes affects longevity, development, and fecundity in the pea aphid (Acyrthosiphon pisum). In Archives of insect biochemistry and physiology, 103, e21614. doi:10.1002/arch.21614. https://pubmed.ncbi.nlm.nih.gov/31498475/
4. Suganuma, Tamaki, Gutiérrez, José L, Li, Bing, Abmayr, Susan M, Workman, Jerry L. 2008. ATAC is a double histone acetyltransferase complex that stimulates nucleosome sliding. In Nature structural & molecular biology, 15, 364-72. doi:10.1038/nsmb.1397. https://pubmed.ncbi.nlm.nih.gov/18327268/
5. Roza, Mauricio, Eriksson, Andreas N M, Svanholm, Sofie, Berg, Cecilia, Karlsson, Oskar. 2024. Male-transmitted transgenerational effects of the herbicide linuron on DNA methylation profiles in Xenopus tropicalis brain and testis. In The Science of the total environment, 923, 170949. doi:10.1016/j.scitotenv.2024.170949. https://pubmed.ncbi.nlm.nih.gov/38365020/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen