C57BL/6JCya-Tm4sf4em1flox/Cya
Common Name:
Tm4sf4-flox
Product ID:
S-CKO-07238
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tm4sf4-flox
Strain ID
CKOCMP-229302-Tm4sf4-B6J-VA
Gene Name
Product ID
S-CKO-07238
Gene Alias
Iltmp
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tm4sf4em1flox/Cya mice (Catalog S-CKO-07238) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029377
NCBI RefSeq
NM_145539
Target Region
Exon 3
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Tm4sf4, a member of the transmembrane 4 superfamily, has physiological functions in regulating tissue differentiation, signal transduction pathways, cellular activation, proliferation, motility, adhesion, and angiogenesis [4]. It is involved in multiple pathways, such as the AKT pathway [1], and has been associated with the regulation of cell junction and cohesion processes [1]. Genetic models are valuable for studying Tm4sf4's role in various biological processes and diseases.
In papillary thyroid cancer (PTC), Tm4sf4 is significantly upregulated and serves as a diagnostic biomarker. Downregulation of Tm4sf4 in vitro suppressed the proliferation and metastasis of PTC cell lines while inducing apoptosis, and the AKT activator could reverse these effects, suggesting Tm4sf4 promotes PTC progression via the AKT pathway [1]. In lung and breast cancers, Tm4sf4 was identified as a potential therapeutic target through outlier analysis, and its knockdown suppressed the growth of lung cancer cell lines [2]. In hepatocellular carcinoma, Tm4sf4 was highly expressed, and the lncRNA ST8SIA6-AS1 regulated its expression through the miR-651-5p/Tm4sf4 axis, affecting cancer cell proliferation and migration [3]. In radiation-resistant lung adenocarcinoma cells, Tm4sf4 was highly expressed, activating cell growth, migration, and invasion via IGF1-induced IGF1R activation [5]. In colorectal cancer, Tm4sf4 was the downstream target of TYMS, and its overexpression increased the migration and invasion of CRC cells by regulating EMT and CD133 expression [6]. Adenovirus-mediated delivery of siRNA targeting Tm4sf4 attenuated liver cancer cell growth in vitro and in vivo [7].
In conclusion, Tm4sf4 plays crucial roles in the progression of multiple cancers, including papillary thyroid cancer, lung cancer, breast cancer, hepatocellular carcinoma, and colorectal cancer. Studies using gene knockdown or knockout-like models (such as siRNA-mediated knockdown) have revealed its oncogenic functions, making it a potential target for cancer diagnosis and treatment.
References：
1. Lin, Lizhi, Wen, Jialiang, Xu, Tiansheng, Si, Yuhao. 2024. TM4SF4 is a diagnostic biomarker accelerating progression of papillary thyroid cancer via AKT pathway. In Cancer biology & therapy, 25, 2424570. doi:10.1080/15384047.2024.2424570. https://pubmed.ncbi.nlm.nih.gov/39497261/
2. Jung, Kyungsoo, Choi, Joon-Seok, Koo, Beom-Mo, Oh, Doo-Yi, Choi, Yoon-La. 2020. TM4SF4 and LRRK2 Are Potential Therapeutic Targets in Lung and Breast Cancers through Outlier Analysis. In Cancer research and treatment, 53, 9-24. doi:10.4143/crt.2020.434. https://pubmed.ncbi.nlm.nih.gov/32972043/
3. Mou, Yanjie, Ding, Xiaoming. 2022. LncRNA ST8SIA6-AS1 facilitates hepatocellular carcinoma progression by governing miR-651-5p/TM4SF4 axis. In Anti-cancer drugs, 33, 741-751. doi:10.1097/CAD.0000000000001326. https://pubmed.ncbi.nlm.nih.gov/35946523/
4. Rahim, Nur Syafiqah, Wu, Yuan Seng, Sim, Maw Shin, Sekar, Mahendran, Guad, Rhanye Mac. 2023. Three Members of Transmembrane-4-Superfamily, TM4SF1, TM4SF4, and TM4SF5, as Emerging Anticancer Molecular Targets against Cancer Phenotypes and Chemoresistance. In Pharmaceuticals (Basel, Switzerland), 16, . doi:10.3390/ph16010110. https://pubmed.ncbi.nlm.nih.gov/36678607/
5. Choi, Soo-Im, Kim, Seo-Yeon, Lee, Jaeha, Cho, Eun-Wie, Kim, In-Gyu. . TM4SF4 overexpression in radiation-resistant lung carcinoma cells activates IGF1R via elevation of IGF1. In Oncotarget, 5, 9823-37. doi:. https://pubmed.ncbi.nlm.nih.gov/25344917/
6. Zhang, Fen, Ye, Jiecheng, Guo, Wenjing, Wang, Liyuan, Han, Anjia. 2022. TYMS-TM4SF4 axis promotes the progression of colorectal cancer by EMT and upregulating stem cell marker. In American journal of cancer research, 12, 1009-1026. doi:. https://pubmed.ncbi.nlm.nih.gov/35411242/
7. Wang, Leiming, Feng, Jian, Da, Liang, Li, Zaiping, Zhao, Mujun. 2013. Adenovirus-mediated delivery of siRNA targeting TM4SF4 attenuated liver cancer cell growth in vitro and in vivo. In Acta biochimica et biophysica Sinica, 45, 213-9. doi:10.1093/abbs/gms115. https://pubmed.ncbi.nlm.nih.gov/23296076/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen