C57BL/6JCya-Fhdc1em1flox/Cya
Common Name:
Fhdc1-flox
Product ID:
S-CKO-07247
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fhdc1-flox
Strain ID
CKOCMP-229474-Fhdc1-B6J-VA
Gene Name
Product ID
S-CKO-07247
Gene Alias
6330505N24Rik; Gm126
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fhdc1em1flox/Cya mice (Catalog S-CKO-07247) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000107689
NCBI RefSeq
NM_001033301
Target Region
Exon 4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Fhdc1, also known as INF1, is a non-FDD-type Formin homology protein with FH1 and FH2 domains in the N-terminal part and FDCH1-FDCH5 domains in the C-terminal part [2]. It is crucial for coordinating the dynamics of actin and microtubule networks, which is essential for many cellular processes such as cell division, migration, Golgi ribbon formation, and cilia assembly [1,3,4].
Knocking down FHDC1 inhibits actin wave formation in mast cells, indicating its role as an early regulator in formin-mediated actin polymerization [1]. In mammalian cells, knockdown of FHDC1 expression results in defective Golgi assembly, suggesting its importance in maintaining the Golgi-derived microtubule network [3]. Overexpression of FHDC1 affects ciliogenesis, with most cells showing blocked cilia assembly while the present cilia are elongated, and FHDC1 depletion inhibits cilia assembly and induces cilia resorption [4].
In conclusion, Fhdc1 is vital for coordinating cytoskeletal dynamics in multiple cellular processes. The findings from knockdown experiments, which can be considered as a form of loss-of-function study similar to gene knockout in principle, have revealed its significance in actin-microtubule crosstalk, Golgi morphology regulation, and cilia length control. This understanding of Fhdc1's functions may provide insights into related cellular malfunctions and potential disease mechanisms [1,3,4].
References:
1. Tong, Chee San, Su, Maohan, Sun, He, Miao, Yansong, Wu, Min. 2024. Collective dynamics of actin and microtubule and its crosstalk mediated by FHDC1. In Frontiers in cell and developmental biology, 11, 1261117. doi:10.3389/fcell.2023.1261117. https://pubmed.ncbi.nlm.nih.gov/38567385/
2. Katoh, Masuko, Katoh, Masaru. . Identification and characterization of human FHDC1, mouse Fhdc1 and zebrafish fhdc1 genes in silico. In International journal of molecular medicine, 13, 929-34. doi:. https://pubmed.ncbi.nlm.nih.gov/15138637/
3. Copeland, Sarah J, Thurston, Susan F, Copeland, John W. 2015. Actin- and microtubule-dependent regulation of Golgi morphology by FHDC1. In Molecular biology of the cell, 27, 260-76. doi:10.1091/mbc.E15-02-0070. https://pubmed.ncbi.nlm.nih.gov/26564798/
4. Copeland, Sarah J, McRae, Andrea, Guarguaglini, Giulia, Trinkle-Mulcahy, Laura, Copeland, John W. 2018. Actin-dependent regulation of cilia length by the inverted formin FHDC1. In Molecular biology of the cell, 29, 1611-1627. doi:10.1091/mbc.E18-02-0088. https://pubmed.ncbi.nlm.nih.gov/29742020/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen