C57BL/6JCya-Ints3em1flox/Cya
Common Name:
Ints3-flox
Product ID:
S-CKO-07259
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ints3-flox
Strain ID
CKOCMP-229543-Ints3-B6J-VA
Gene Name
Product ID
S-CKO-07259
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ints3em1flox/Cya mice (Catalog S-CKO-07259) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029542
NCBI RefSeq
NM_145540
Target Region
Exon 4~5
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
INTS3, a subunit of the integrator complex, plays crucial roles in multiple biological processes. It is involved in DNA repair, particularly in homologous recombination-dependent repair of double-strand breaks (DSBs) and in ATM-dependent signaling pathways [1,5,6,7,8,9]. It also has a role in RNA-related functions such as RNA polymerase II termination [4]. Additionally, abnormal INTS3 splicing and reduced expression are associated with leukaemogenesis [2], and it functions as an anti-apoptotic RNA-binding protein in colorectal cancer [3].
CRISPR-Cas9 screening in colorectal cancer cells showed that deletion/depletion of INTS3 triggered apoptosis, destabilized pro-apoptotic gene transcripts, and delayed cell growth in vivo, highlighting its importance in cancer cell survival [3]. In the context of DNA damage response, the INTS3-MISE-hSSB1 complex is key for ATM activation and RAD51 recruitment to DNA damage foci [5]. RPA70 depletion leads to the formation of sub-nuclear foci by hSSB1 and INTS3, which recruit the ATR-ATRIP checkpoint complex to sites of genomic stress [7]. Perturbation of INTS3 dimerization and disruption of the INTS3/INTS6 interaction impair the DSB repair process [9].
In summary, INTS3 is essential for DNA repair, regulation of apoptosis in cancer cells, and has implications in leukaemogenesis. Studies using loss-of-function models like CRISPR-Cas9 knockout in cancer cells and investigations of protein-protein interactions related to INTS3 have provided valuable insights into its role in these biological processes and disease conditions.
References:
1. Li, Jian, Ma, Xinli, Banerjee, Surajit, Bode, Ann M, Dong, Zigang. 2020. Structural basis for multifunctional roles of human Ints3 C-terminal domain. In The Journal of biological chemistry, 296, 100112. doi:10.1074/jbc.RA120.016393. https://pubmed.ncbi.nlm.nih.gov/33434574/
2. Yoshimi, Akihide, Lin, Kuan-Ting, Wiseman, Daniel H, Krainer, Adrian R, Abdel-Wahab, Omar. 2019. Coordinated alterations in RNA splicing and epigenetic regulation drive leukaemogenesis. In Nature, 574, 273-277. doi:10.1038/s41586-019-1618-0. https://pubmed.ncbi.nlm.nih.gov/31578525/
3. Wang, Zhiwei, Zhang, Cheng, Guo, Jing, Zhu, Pingping, He, Qiankun. 2024. CRISPR-Cas9 screening identifies INTS3 as an anti-apoptotic RNA-binding protein and therapeutic target for colorectal cancer. In iScience, 27, 109676. doi:10.1016/j.isci.2024.109676. https://pubmed.ncbi.nlm.nih.gov/38665208/
4. Fianu, Isaac, Ochmann, Moritz, Walshe, James L, Urlaub, Henning, Cramer, Patrick. 2024. Structural basis of Integrator-dependent RNA polymerase II termination. In Nature, 629, 219-227. doi:10.1038/s41586-024-07269-4. https://pubmed.ncbi.nlm.nih.gov/38570683/
5. Skaar, Jeffrey R, Richard, Derek J, Saraf, Anita, Khanna, Kum Kum, Pagano, Michele. 2009. INTS3 controls the hSSB1-mediated DNA damage response. In The Journal of cell biology, 187, 25-32. doi:10.1083/jcb.200907026. https://pubmed.ncbi.nlm.nih.gov/19786574/
6. Barbhuiya, Tabassum Khair, Beard, Sam, Shah, Esha T, Adams, Mark N, Gandhi, Neha S. 2024. Targeting the hSSB1-INTS3 Interface: A Computational Screening Driven Approach to Identify Potential Modulators. In ACS omega, 9, 8362-8373. doi:10.1021/acsomega.3c09267. https://pubmed.ncbi.nlm.nih.gov/38405517/
7. Kar, Ananya, Kaur, Manpreet, Ghosh, Tanushree, Varshney, Akhil, Saxena, Sandeep. 2015. RPA70 depletion induces hSSB1/2-INTS3 complex to initiate ATR signaling. In Nucleic acids research, 43, 4962-74. doi:10.1093/nar/gkv369. https://pubmed.ncbi.nlm.nih.gov/25916848/
8. Long, Qilin, Sebesta, Marek, Sedova, Katerina, Stefl, Richard, Gullerova, Monika. 2023. The phosphorylated trimeric SOSS1 complex and RNA polymerase II trigger liquid-liquid phase separation at double-strand breaks. In Cell reports, 42, 113489. doi:10.1016/j.celrep.2023.113489. https://pubmed.ncbi.nlm.nih.gov/38039132/
9. Jia, Yu, Cheng, Zixiu, Bharath, Sakshibeedu R, Huang, Jun, Song, Haiwei. 2021. Crystal structure of the INTS3/INTS6 complex reveals the functional importance of INTS3 dimerization in DSB repair. In Cell discovery, 7, 66. doi:10.1038/s41421-021-00283-0. https://pubmed.ncbi.nlm.nih.gov/34400606/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen