Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Ints3em1flox/Cya
Common Name:
Ints3-flox
Product ID:
S-CKO-07259
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ints3-flox
Strain ID
CKOCMP-229543-Ints3-B6J-VA
Gene Name
Ints3
Product ID
S-CKO-07259
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
229543
Modification
Conditional knockout
Chromosome
3
Phenotype
MGI:2140050
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ints3em1flox/Cya mice (Catalog S-CKO-07259) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029542
NCBI RefSeq
NM_145540
Target Region
Exon 4~5
Size of Effective Region
~1.3 kb
Detailed Document
Click here to download >>
Overview of Gene Research
INTS3, a subunit of the integrator complex, plays crucial roles in multiple biological processes. It is involved in DNA repair, particularly in homologous recombination-dependent repair of double-strand breaks (DSBs) and in ATM-dependent signaling pathways [1,5,6,7,8,9]. It also has a role in RNA-related functions such as RNA polymerase II termination [4]. Additionally, abnormal INTS3 splicing and reduced expression are associated with leukaemogenesis [2], and it functions as an anti-apoptotic RNA-binding protein in colorectal cancer [3].

CRISPR-Cas9 screening in colorectal cancer cells showed that deletion/depletion of INTS3 triggered apoptosis, destabilized pro-apoptotic gene transcripts, and delayed cell growth in vivo, highlighting its importance in cancer cell survival [3]. In the context of DNA damage response, the INTS3-MISE-hSSB1 complex is key for ATM activation and RAD51 recruitment to DNA damage foci [5]. RPA70 depletion leads to the formation of sub-nuclear foci by hSSB1 and INTS3, which recruit the ATR-ATRIP checkpoint complex to sites of genomic stress [7]. Perturbation of INTS3 dimerization and disruption of the INTS3/INTS6 interaction impair the DSB repair process [9].

In summary, INTS3 is essential for DNA repair, regulation of apoptosis in cancer cells, and has implications in leukaemogenesis. Studies using loss-of-function models like CRISPR-Cas9 knockout in cancer cells and investigations of protein-protein interactions related to INTS3 have provided valuable insights into its role in these biological processes and disease conditions.

References:
1. Li, Jian, Ma, Xinli, Banerjee, Surajit, Bode, Ann M, Dong, Zigang. 2020. Structural basis for multifunctional roles of human Ints3 C-terminal domain. In The Journal of biological chemistry, 296, 100112. doi:10.1074/jbc.RA120.016393. https://pubmed.ncbi.nlm.nih.gov/33434574/
2. Yoshimi, Akihide, Lin, Kuan-Ting, Wiseman, Daniel H, Krainer, Adrian R, Abdel-Wahab, Omar. 2019. Coordinated alterations in RNA splicing and epigenetic regulation drive leukaemogenesis. In Nature, 574, 273-277. doi:10.1038/s41586-019-1618-0. https://pubmed.ncbi.nlm.nih.gov/31578525/
3. Wang, Zhiwei, Zhang, Cheng, Guo, Jing, Zhu, Pingping, He, Qiankun. 2024. CRISPR-Cas9 screening identifies INTS3 as an anti-apoptotic RNA-binding protein and therapeutic target for colorectal cancer. In iScience, 27, 109676. doi:10.1016/j.isci.2024.109676. https://pubmed.ncbi.nlm.nih.gov/38665208/
4. Fianu, Isaac, Ochmann, Moritz, Walshe, James L, Urlaub, Henning, Cramer, Patrick. 2024. Structural basis of Integrator-dependent RNA polymerase II termination. In Nature, 629, 219-227. doi:10.1038/s41586-024-07269-4. https://pubmed.ncbi.nlm.nih.gov/38570683/
5. Skaar, Jeffrey R, Richard, Derek J, Saraf, Anita, Khanna, Kum Kum, Pagano, Michele. 2009. INTS3 controls the hSSB1-mediated DNA damage response. In The Journal of cell biology, 187, 25-32. doi:10.1083/jcb.200907026. https://pubmed.ncbi.nlm.nih.gov/19786574/
6. Barbhuiya, Tabassum Khair, Beard, Sam, Shah, Esha T, Adams, Mark N, Gandhi, Neha S. 2024. Targeting the hSSB1-INTS3 Interface: A Computational Screening Driven Approach to Identify Potential Modulators. In ACS omega, 9, 8362-8373. doi:10.1021/acsomega.3c09267. https://pubmed.ncbi.nlm.nih.gov/38405517/
7. Kar, Ananya, Kaur, Manpreet, Ghosh, Tanushree, Varshney, Akhil, Saxena, Sandeep. 2015. RPA70 depletion induces hSSB1/2-INTS3 complex to initiate ATR signaling. In Nucleic acids research, 43, 4962-74. doi:10.1093/nar/gkv369. https://pubmed.ncbi.nlm.nih.gov/25916848/
8. Long, Qilin, Sebesta, Marek, Sedova, Katerina, Stefl, Richard, Gullerova, Monika. 2023. The phosphorylated trimeric SOSS1 complex and RNA polymerase II trigger liquid-liquid phase separation at double-strand breaks. In Cell reports, 42, 113489. doi:10.1016/j.celrep.2023.113489. https://pubmed.ncbi.nlm.nih.gov/38039132/
9. Jia, Yu, Cheng, Zixiu, Bharath, Sakshibeedu R, Huang, Jun, Song, Haiwei. 2021. Crystal structure of the INTS3/INTS6 complex reveals the functional importance of INTS3 dimerization in DSB repair. In Cell discovery, 7, 66. doi:10.1038/s41421-021-00283-0. https://pubmed.ncbi.nlm.nih.gov/34400606/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest