C57BL/6JCya-Csde1em1flox/Cya
Common Name:
Csde1-flox
Product ID:
S-CKO-07271
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Csde1-flox
Strain ID
CKOCMP-229663-Csde1-B6J-VA
Gene Name
Product ID
S-CKO-07271
Gene Alias
D3Jfr1; mKIAA0885; unr
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Csde1em1flox/Cya mice (Catalog S-CKO-07271) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029446
NCBI RefSeq
NM_144901
Target Region
Exon 4~5
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Csde1, also known as cold shock domain containing E1, is an RNA-binding protein that plays a key role in translational reprogramming. It can promote or repress RNA translation and influence RNA abundance [2]. Csde1 is involved in multiple biological pathways, such as the miRNA-mediated gene regulation pathway and Wnt/β-catenin signaling pathway, and is important for biological processes like hematopoiesis, erythrocyte development, and may be relevant in cancer development [1,2,3,4]. Genetic models are valuable for studying Csde1's function.
In zebrafish, depletion of csde1 in genetic mutants and morphants impaired the production of hematopoietic stem and progenitor cells (HSPCs), as Csde1 regulates HSPC generation by modulating Wnt/β-catenin signaling activity, specifically by binding to ctnnb1 mRNAs and regulating their translation [4]. In mouse models, depletion of CSDE1 in primary mouse keratinocytes led to senescence bypass, cell immortalization, and tumor formation, indicating its role as a potential tumor suppressor in oncogene-induced senescence (OIS) [5].
In conclusion, Csde1 is a crucial regulator in translational reprogramming and is involved in multiple biological processes. Model-based research, especially the use of gene-knockout models in zebrafish and mice, has revealed its roles in hematopoiesis and oncogene-induced senescence, providing insights into related disease mechanisms such as cancer development.
References:
1. Kakumani, Pavan Kumar, Ko, Yunkoo, Ramakrishna, Sushmitha, Shin, Chanseok, Simard, Martin J. . CSDE1 promotes miR-451 biogenesis. In Nucleic acids research, 51, 9385-9396. doi:10.1093/nar/gkad619. https://pubmed.ncbi.nlm.nih.gov/37493604/
2. Guo, Ao-Xiang, Cui, Jia-Jia, Wang, Lei-Yun, Yin, Ji-Ye. 2020. The role of CSDE1 in translational reprogramming and human diseases. In Cell communication and signaling : CCS, 18, 14. doi:10.1186/s12964-019-0496-2. https://pubmed.ncbi.nlm.nih.gov/31987048/
3. Ciocia, Annagiulia, Mestre-Farràs, Neus, Vicent-Nacht, Ignacio, Guitart, Tanit, Gebauer, Fátima. 2024. CSDE1: a versatile regulator of gene expression in cancer. In NAR cancer, 6, zcae014. doi:10.1093/narcan/zcae014. https://pubmed.ncbi.nlm.nih.gov/38600987/
4. Li, Ying, Li, Can, Liu, Mengyao, Liu, Feng, Wang, Lu. 2023. The RNA-binding protein CSDE1 promotes hematopoietic stem and progenitor cell generation via translational control of Wnt signaling. In Development (Cambridge, England), 150, . doi:10.1242/dev.201890. https://pubmed.ncbi.nlm.nih.gov/37874038/
5. Avolio, Rosario, Inglés-Ferrándiz, Marta, Ciocia, Annagiulia, Ribó, Anna, Gebauer, Fátima. . Coordinated post-transcriptional control of oncogene-induced senescence by UNR/CSDE1. In Cell reports, 38, 110211. doi:10.1016/j.celrep.2021.110211. https://pubmed.ncbi.nlm.nih.gov/35021076/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen