C57BL/6NCya-Rbm15em1flox/Cya
Common Name:
Rbm15-flox
Product ID:
S-CKO-07280
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rbm15-flox
Strain ID
CKOCMP-229700-Rbm15-B6N-VA
Gene Name
Product ID
S-CKO-07280
Gene Alias
C230088J01Rik; mKIAA1438
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Rbm15em1flox/Cya mice (Catalog S-CKO-07280) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000061772
NCBI RefSeq
NM_001045807
Target Region
Exon 1
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
Rbm15, an RNA-binding protein, is a crucial "writer" of the m6A methyltransferase complex. N6-methyladenosine (m6A) is a prevalent and conserved internal cotranscriptional modification in eukaryotic RNAs. Rbm15 regulates multiple signal pathways such as Notch and Wnt, playing important roles in cell growth, apoptosis, especially in blood cells [3].
In laryngeal squamous cell carcinoma (LSCC), knockdown of Rbm15 reduced cell proliferation, invasion, migration, and promoted apoptosis both in vitro and in vivo, as it regulates TMBIM6 stability through an IGF2BP3-dependent mechanism [1]. In lung adenocarcinoma, overexpression of Rbm15 increased m6A levels and promoted cell invasion, migration, and proliferation, while knockdown had opposite effects. Rbm15-mediated m6A modification inhibits RASSF8 protein levels to increase cell invasion and migration [2]. In triple-negative breast cancer, Rbm15 drives cell growth by stimulating serine and glycine metabolism [4]. In lung cancer, Rbm15 silencing promoted ferroptosis by regulating the TGF-β/Smad2 pathway, reducing cell viability, proliferation, invasion, and migration [5]. In lung adenocarcinoma, Rbm15 overexpression facilitated osimertinib resistance by enhancing m6A modification of SPOCK1 mRNA [6].
In conclusion, Rbm15 is significantly involved in the development and progression of multiple cancers, playing a key role in processes like cell proliferation, invasion, migration, apoptosis, and drug resistance. The study of Rbm15 through gene-knockout or knockdown models in these cancer-related disease areas has provided valuable insights into its functions, offering potential therapeutic targets for cancer treatment.
References:
1. Wang, Xin, Tian, Linli, Li, Yushan, Wang, Peng, Sun, Yanan. 2021. RBM15 facilitates laryngeal squamous cell carcinoma progression by regulating TMBIM6 stability through IGF2BP3 dependent. In Journal of experimental & clinical cancer research : CR, 40, 80. doi:10.1186/s13046-021-01871-4. https://pubmed.ncbi.nlm.nih.gov/33637103/
2. Ma, Mingsheng, Wang, Wei, Li, Li, Zhao, Guangqiang, Ye, Lianhua. 2024. RBM15 facilities lung adenocarcinoma cell progression by regulating RASSF8 stability through N6 Methyladenosine modification. In Translational oncology, 46, 102018. doi:10.1016/j.tranon.2024.102018. https://pubmed.ncbi.nlm.nih.gov/38838436/
3. Hu, Mengyu, Yang, Yuxia, Ji, Zhi, Luo, Jianyuan. . RBM15 Functions in Blood Diseases. In Current cancer drug targets, 16, 579-85. doi:. https://pubmed.ncbi.nlm.nih.gov/26758534/
4. Park, Su Hwan, Ju, Jin-Sung, Woo, Hyunmin, Lee, Jong-Ho, Park, Yun-Yong. 2024. The m6A writer RBM15 drives the growth of triple-negative breast cancer cells through the stimulation of serine and glycine metabolism. In Experimental & molecular medicine, 56, 1373-1387. doi:10.1038/s12276-024-01235-w. https://pubmed.ncbi.nlm.nih.gov/38825643/
5. Feng, Jing, Li, Yaling, He, Fen, Zhang, Fuwei. 2023. RBM15 silencing promotes ferroptosis by regulating the TGF-β/Smad2 pathway in lung cancer. In Environmental toxicology, 38, 950-961. doi:10.1002/tox.23741. https://pubmed.ncbi.nlm.nih.gov/36715115/
6. Li, Hongxiang, Li, Yin, Zheng, Xiaoxiao, Cheng, Xinghua, Xu, Guodong. 2024. RBM15 facilitates osimertinib resistance of lung adenocarcinoma through m6A-dependent epigenetic silencing of SPOCK1. In Oncogene, 44, 307-321. doi:10.1038/s41388-024-03220-z. https://pubmed.ncbi.nlm.nih.gov/39528815/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen