C57BL/6JCya-Strip1em1flox/Cya
Common Name:
Strip1-flox
Product ID:
S-CKO-07282
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Strip1-flox
Strain ID
CKOCMP-229707-Strip1-B6J-VA
Gene Name
Product ID
S-CKO-07282
Gene Alias
6330569M22Rik; 6530401O14Rik; Fam40a
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Strip1em1flox/Cya mice (Catalog S-CKO-07282) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000064759
NCBI RefSeq
NM_153563
Target Region
Exon 2~5
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Strip1, or striatin-interacting protein 1, is a core component of the striatin-interacting phosphatases and kinases (STRIPAK) complex. This complex integrates diverse cellular signals in the Hippo pathway to regulate cell proliferation and survival [6]. Strip1 has shown to be important in embryonic morphogenesis, and genetic models like zebrafish, C. elegans, and mice have been used to study its functions [1,3,5].
In zebrafish, loss of Strip1 causes defects in inner retinal circuit formation. RGCs undergo apoptosis, and the inner plexiform layer becomes disorganized, suggesting Strip1 promotes RGC survival and proper neuron positioning in the inner retina [1].
In C. elegans, FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization. Missense mutations in farl-11 lead to sarcomere disorganization and SR disruption [3,4].
In mice, Strip1-null mutants arrest development at mid-gestation with mesoderm migration defects, abnormal focal adhesions, and actin cytoskeleton changes [5]. In mouse cochlear hair cells, Strip1 expression increases with age, and homozygous knockout is embryonic lethal, suggesting its importance in hair cell development [2].
In breast cancer cells, loss of STRIP1 leads to cell cycle arrest and decreased proliferation, yet may allow escape from therapy-induced senescence [7].
In summary, Strip1 is crucial for various biological processes such as embryonic development, inner retina formation, muscle structure organization, and cochlear hair cell development. Studies using gene knockout models in different organisms have revealed its roles in these processes and potential implications in diseases like glaucoma and breast cancer.
References:
1. Ahmed, Mai, Kojima, Yutaka, Masai, Ichiro. 2022. Strip1 regulates retinal ganglion cell survival by suppressing Jun-mediated apoptosis to promote retinal neural circuit formation. In eLife, 11, . doi:10.7554/eLife.74650. https://pubmed.ncbi.nlm.nih.gov/35314028/
2. Zhang, Shasha, Dong, Ying, Qiang, Ruiying, Chen, Jie, Chai, Renjie. 2021. Characterization of Strip1 Expression in Mouse Cochlear Hair Cells. In Frontiers in genetics, 12, 625867. doi:10.3389/fgene.2021.625867. https://pubmed.ncbi.nlm.nih.gov/33889175/
3. Martin, Sterling C T, Qadota, Hiroshi, Oberhauser, Andres F, Hardin, Jeff, Benian, Guy M. 2023. FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in C. elegans. In Molecular biology of the cell, 34, ar86. doi:10.1091/mbc.E23-03-0083. https://pubmed.ncbi.nlm.nih.gov/37314837/
4. Martin, Sterling C T, Qadota, Hiroshi, Oberhauser, Andres F, Hardin, Jeff, Benian, Guy M. 2023. FARL-11 (STRIP1/2) is Required for Sarcomere and Sarcoplasmic Reticulum Organization in C. elegans. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.03.05.531173. https://pubmed.ncbi.nlm.nih.gov/36945551/
5. Bazzi, Hisham, Soroka, Ekaterina, Alcorn, Heather L, Anderson, Kathryn V. 2017. STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo. In Proceedings of the National Academy of Sciences of the United States of America, 114, E10928-E10936. doi:10.1073/pnas.1713535114. https://pubmed.ncbi.nlm.nih.gov/29203676/
6. Jeong, Byung-Cheon, Bae, Sung Jun, Ni, Lisheng, Bai, Xiao-Chen, Luo, Xuelian. 2021. Cryo-EM structure of the Hippo signaling integrator human STRIPAK. In Nature structural & molecular biology, 28, 290-299. doi:10.1038/s41594-021-00564-y. https://pubmed.ncbi.nlm.nih.gov/33633399/
7. Rodriguez-Cupello, Carmen, Dam, Monica, Serini, Laura, García-Mariscal, Alberto, Madsen, Chris D. 2020. The STRIPAK Complex Regulates Response to Chemotherapy Through p21 and p27. In Frontiers in cell and developmental biology, 8, 146. doi:10.3389/fcell.2020.00146. https://pubmed.ncbi.nlm.nih.gov/32258031/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen