C57BL/6JCya-Cenpeem1flox/Cya
Common Name:
Cenpe-flox
Product ID:
S-CKO-07295
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cenpe-flox
Strain ID
CKOCMP-229841-Cenpe-B6J-VA
Gene Name
Product ID
S-CKO-07295
Gene Alias
312kDa; C530022J18; CENP-E; Kif10
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cenpeem1flox/Cya mice (Catalog S-CKO-07295) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000062893
NCBI RefSeq
NM_173762
Target Region
Exon 2~5
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Cenpe, short for centromeric protein E, is a microtubule plus-end-directed kinetochore protein, belonging to the centromere protein (CENP) family. It plays a crucial role in mitosis and is involved in cell cycle-related processes. Heterozygous mutations in Cenpe can lead to primary microcephaly syndrome [1,4].
In non-WNT/non-SHH medulloblastoma, Cenpe is highly expressed compared to normal tissues and serves as an independent prognostic factor for survival. Knockdown of Cenpe inhibits cell proliferation by activating the p53 signaling pathway and blocking the cell cycle [1].
In pulmonary hypertension, Cenpe deficiency significantly inhibits the development of pulmonary vascular remodeling and right ventricular hypertrophy, and knocking out Cenpe effectively inhibits the proliferation and induces apoptosis of primary pulmonary artery smooth muscle cells [2].
In cervical cancer, Cenpe expression is upregulated, promoting the progression of cervical squamous cell carcinoma through IL-6-mediated PI3K-Akt and MAPK signaling pathways [3].
In medulloblastoma cells, CENPE knockdown induces mitotic defects, apoptosis, and endogenous DNA damage accumulation, activating TP53 or TP73 as well as cell death signaling pathways [4].
In glioblastoma, Cenpe promotes proliferation by directly binding to WEE1 and regulating the G0-G1 and G2/M phase transitions [5].
In chemoresistant acute myeloid leukemia, knockdown of Cenpe expression suppresses the proliferation of myeloid leukemia cells and reverses cytarabine chemoresistance [6].
In esophageal adenocarcinoma, high Cenpe expression is associated with unfavorable overall survival, and its expression is related to DNA methylation status [7].
In chromophobe renal cell carcinoma, Cenpe is highly expressed and is an important prognostic biomarker, and its function is mainly enriched in proliferation-related pathways [8].
In non-small cell lung cancer, high expression of Cenpe is related to poor prognosis [9].
In lung adenocarcinoma, Cenpe promotes proliferation and is directly regulated by FOXM1 [10].
In conclusion, Cenpe is a key protein in cell cycle-related processes and mitosis. Research using gene-knockout or knockdown models has revealed its significance in various diseases, including different types of cancers and pulmonary hypertension. These findings suggest that Cenpe could be a potential biomarker and therapeutic target for these diseases.
References:
1. Fang, Huangyi, Zhang, Yusong, Lin, Chengyin, Sheng, Hansong, Lin, Jian. 2023. Primary microcephaly gene CENPE is a novel biomarker and potential therapeutic target for non-WNT/non-SHH medulloblastoma. In Frontiers in immunology, 14, 1227143. doi:10.3389/fimmu.2023.1227143. https://pubmed.ncbi.nlm.nih.gov/37593739/
2. Fang, Xiaoyu, Xie, Min, Liu, Xiansheng, He, Yuanzhou. 2021. CENPE contributes to pulmonary vascular remodeling in pulmonary hypertension. In Biochemical and biophysical research communications, 557, 40-47. doi:10.1016/j.bbrc.2021.04.010. https://pubmed.ncbi.nlm.nih.gov/33862458/
3. Peng, Peiqiang, Zheng, Jingying, He, Kang, Zhang, Shuang, Zhao, Lijing. 2024. CENPE is a diagnostic and prognostic biomarker for cervical cancer. In Heliyon, 10, e40860. doi:10.1016/j.heliyon.2024.e40860. https://pubmed.ncbi.nlm.nih.gov/39759304/
4. Iegiani, Giorgia, Gai, Marta, Di Cunto, Ferdinando, Pallavicini, Gianmarco. 2021. CENPE Inhibition Leads to Mitotic Catastrophe and DNA Damage in Medulloblastoma Cells. In Cancers, 13, . doi:10.3390/cancers13051028. https://pubmed.ncbi.nlm.nih.gov/33804489/
5. Ma, Chuanling, Wang, Jianchao, Zhou, Jie, Li, Fangqiong, Zhang, Meng. . CENPE promotes glioblastomas proliferation by directly binding to WEE1. In Translational cancer research, 9, 717-725. doi:10.21037/tcr.2019.11.40. https://pubmed.ncbi.nlm.nih.gov/35117417/
6. Shi, Mingyue, Niu, Junwei, Niu, Xiaona, Chen, Yuqing, Shao, Fengmin. 2021. Lin28A/CENPE Promoting the Proliferation and Chemoresistance of Acute Myeloid Leukemia. In Frontiers in oncology, 11, 763232. doi:10.3389/fonc.2021.763232. https://pubmed.ncbi.nlm.nih.gov/34868981/
7. Zhu, Xueqiang, Luo, Xing, Feng, Gang, Zeng, Ming, Liu, Hao. 2019. CENPE expression is associated with its DNA methylation status in esophageal adenocarcinoma and independently predicts unfavorable overall survival. In PloS one, 14, e0207341. doi:10.1371/journal.pone.0207341. https://pubmed.ncbi.nlm.nih.gov/30716092/
8. Wu, Hui-Feng, Liu, Hao, Zhang, Zhe-Wei, Chen, Ji-Min. 2023. CENPE and LDHA were potential prognostic biomarkers of chromophobe renal cell carcinoma. In European journal of medical research, 28, 481. doi:10.1186/s40001-023-01449-0. https://pubmed.ncbi.nlm.nih.gov/37925501/
9. Hao, Xuezhi, Qu, Tao. 2019. Expression of CENPE and its Prognostic Role in Non-small Cell Lung Cancer. In Open medicine (Warsaw, Poland), 14, 497-502. doi:10.1515/med-2019-0053. https://pubmed.ncbi.nlm.nih.gov/31259255/
10. Shan, Lina, Zhao, Minjie, Lu, Ya, Chai, Wenshu, Shi, Xianbao. 2019. CENPE promotes lung adenocarcinoma proliferation and is directly regulated by FOXM1. In International journal of oncology, 55, 257-266. doi:10.3892/ijo.2019.4805. https://pubmed.ncbi.nlm.nih.gov/31115500/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen