C57BL/6JCya-Prdm13em1flox/Cya
Common Name:
Prdm13-flox
Product ID:
S-CKO-07305
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Prdm13-flox
Strain ID
CKOCMP-230025-Prdm13-B6J-VA
Gene Name
Product ID
S-CKO-07305
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prdm13em1flox/Cya mice (Catalog S-CKO-07305) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000095141
NCBI RefSeq
NM_001080771
Target Region
Exon 2~3
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
PRDM13, a transcriptional repressor, is a key regulator in neuronal development. It functions downstream of the transcription factor PTF1A and is involved in specifying neuronal subtypes. In the retina, it contributes to the development of amacrine interneurons, and in the spinal cord, it helps maintain the balance of inhibitory and excitatory neurons [3,4,6,7]. It is also implicated in hindbrain development, with its role in hypothalamic and cerebellar development being of particular interest [1,2].
In zebrafish, loss of prdm13 function leads to a reduction in Purkinje cell numbers and absence of the inferior olive nuclei, highlighting its role in hindbrain development [1]. In Prdm13 -deficient mice, there is a reduction in GABAergic and glycinergic amacrines in the retina, and abnormal visual sensitivities are observed [3]. Prdm13 -deficient mice also display cerebellar hypoplasia, delayed pubertal onset, and a significant reduction in the number of Kisspeptin neurons in the hypothalamus [2]. Additionally, chemogenetic inhibition or knockout of Prdm13+ neurons in the dorsomedial hypothalamus of mice affects sleep-wake patterns [5].
In conclusion, Prdm13 is crucial for neuronal subtype specification, especially in the retina, spinal cord, hypothalamus, and cerebellum. Studies using gene knockout mouse models have revealed its role in diseases such as pontocerebellar hypoplasias, congenital hypogonadotropic hypogonadism, and potentially North Carolina macular dystrophy. Understanding Prdm13's function provides insights into the mechanisms of neuronal development and related disorders.
References:
1. Coolen, Marion, Altin, Nami, Rajamani, Karthyayani, Moutton, Sébastien, Cantagrel, Vincent. 2022. Recessive PRDM13 mutations cause fatal perinatal brainstem dysfunction with cerebellar hypoplasia and disrupt Purkinje cell differentiation. In American journal of human genetics, 109, 909-927. doi:10.1016/j.ajhg.2022.03.010. https://pubmed.ncbi.nlm.nih.gov/35390279/
2. Whittaker, Danielle E, Oleari, Roberto, Gregory, Louise C, Basson, M Albert, Dattani, Mehul T. . A recessive PRDM13 mutation results in congenital hypogonadotropic hypogonadism and cerebellar hypoplasia. In The Journal of clinical investigation, 131, . doi:10.1172/JCI141587. https://pubmed.ncbi.nlm.nih.gov/34730112/
3. Watanabe, Satoshi, Sanuki, Rikako, Sugita, Yuko, Ohsuga, Mizuki, Furukawa, Takahisa. . Prdm13 regulates subtype specification of retinal amacrine interneurons and modulates visual sensitivity. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 35, 8004-20. doi:10.1523/JNEUROSCI.0089-15.2015. https://pubmed.ncbi.nlm.nih.gov/25995483/
4. Goodson, Noah B, Nahreini, Jhenya, Randazzo, Grace, Johnson, Jane E, Brzezinski, Joseph A. 2017. Prdm13 is required for Ebf3+ amacrine cell formation in the retina. In Developmental biology, 434, 149-163. doi:10.1016/j.ydbio.2017.12.003. https://pubmed.ncbi.nlm.nih.gov/29258872/
5. Tsuji, Shogo, Brace, Cynthia S, Yao, Ruiqing, Imai, Shin-Ichiro, Satoh, Akiko. 2023. Sleep-wake patterns are altered with age, Prdm13 signaling in the DMH, and diet restriction in mice. In Life science alliance, 6, . doi:10.26508/lsa.202301992. https://pubmed.ncbi.nlm.nih.gov/37045472/
6. Chang, Joshua C, Meredith, David M, Mayer, Paul R, Ou, Yi-Hung, Johnson, Jane E. . Prdm13 mediates the balance of inhibitory and excitatory neurons in somatosensory circuits. In Developmental cell, 25, 182-95. doi:10.1016/j.devcel.2013.02.015. https://pubmed.ncbi.nlm.nih.gov/23639443/
7. Mona, Bishakha, Uruena, Ana, Kollipara, Rahul K, Chang, Joshua C, Johnson, Jane E. 2017. Repression by PRDM13 is critical for generating precision in neuronal identity. In eLife, 6, . doi:10.7554/eLife.25787. https://pubmed.ncbi.nlm.nih.gov/28850031/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen