C57BL/6JCya-Acer2em1flox/Cya
Common Name:
Acer2-flox
Product ID:
S-CKO-07331
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Acer2-flox
Strain ID
CKOCMP-230379-Acer2-B6J-VA
Gene Name
Product ID
S-CKO-07331
Gene Alias
2410116I05Rik; Asah3l; CRG-L1; maCER2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Acer2em1flox/Cya mice (Catalog S-CKO-07331) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000045224
NCBI RefSeq
NM_139306
Target Region
Exon 2~3
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Acer2, encoding alkaline ceramidase 2, plays a critical role in sphingolipid metabolism. It catalyzes the hydrolysis of ceramide to sphingosine, which is then converted to sphingosine-1-phosphate (S1P). This process is involved in regulating various cellular processes such as proliferation, apoptosis, inflammation, and tumor progression [2].
In diffuse large B-cell lymphoma (DLBCL), YTHDF2 binds to m6A sites on Acer2 mRNA, promoting its stability and expression. Enhanced Acer2 expression hydrolyzes ceramides, disrupting the balance between ceramide and S1P, activating the ERK and PI3K/AKT pathways, and leading to DLBCL tumorigenesis [1].
In adipocytes, Acer2 is a target gene of hypoxia-inducible factor 2α (HIF-2α). Adipocyte HIF-2α deficiency exacerbates atherosclerosis by increasing adipose ceramide levels, while adipose overexpression of Acer2 rescues this effect [3].
In addition, knockout of Acer2 in mice leads to increased susceptibility for lithium-induced diabetes insipidus development [4].
In conclusion, Acer2 is essential in sphingolipid metabolism, influencing multiple biological processes. Gene knockout mouse models have revealed its role in diseases like DLBCL, atherosclerosis, and lithium-induced nephrogenic diabetes insipidus, providing insights into potential targeted therapies for these conditions.
References：
1. Chen, Xiaomin, Lu, Tiange, Ding, Mengfei, Zhou, Xiangxiang, Wang, Xin. 2023. Targeting YTHDF2 inhibits tumorigenesis of diffuse large B-cell lymphoma through ACER2-mediated ceramide catabolism. In Journal of advanced research, 63, 17-33. doi:10.1016/j.jare.2023.10.010. https://pubmed.ncbi.nlm.nih.gov/37865189/
2. Liu, Binggang, Zhou, Junfeng, Jiang, Biao, Liu, Ting, Lei, Pengcheng. 2024. The role of ACER2 in intestinal sphingolipid metabolism and gastrointestinal cancers. In Frontiers in immunology, 15, 1511283. doi:10.3389/fimmu.2024.1511283. https://pubmed.ncbi.nlm.nih.gov/39650647/
3. Zhang, Xingzhong, Zhang, Yangming, Wang, Pengcheng, Wang, Xian, Jiang, Changtao. 2019. Adipocyte Hypoxia-Inducible Factor 2α Suppresses Atherosclerosis by Promoting Adipose Ceramide Catabolism. In Cell metabolism, 30, 937-951.e5. doi:10.1016/j.cmet.2019.09.016. https://pubmed.ncbi.nlm.nih.gov/31668872/
4. de Groot, Theun, Ebert, Lena K, Christensen, Birgitte Mønster, Deen, Peter M T, Korstanje, Ron. 2019. Identification of Acer2 as a First Susceptibility Gene for Lithium-Induced Nephrogenic Diabetes Insipidus in Mice. In Journal of the American Society of Nephrology : JASN, 30, 2322-2336. doi:10.1681/ASN.2018050549. https://pubmed.ncbi.nlm.nih.gov/31558682/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen