C57BL/6JCya-Tut4em1flox/Cya
Common Name:
Tut4-flox
Product ID:
S-CKO-07346
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tut4-flox
Strain ID
CKOCMP-230594-Tut4-B6J-VA
Gene Name
Product ID
S-CKO-07346
Gene Alias
6030404K05Rik; 9230115F04Rik; PPAPD3; Tent3a; Zcchc11; mKIAA0191
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tut4em1flox/Cya mice (Catalog S-CKO-07346) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000043368
NCBI RefSeq
NM_175472
Target Region
Exon 4
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Tut4, also known as Zcchc11, TENT3A, or Z11, is a terminal uridylyltransferase. It plays a crucial role in regulating miRNA and mRNA homeostasis through 3' end uridylation [2,3,4,5,6]. This process is involved in various biological pathways such as controlling the stability of transcripts, and is important for maintaining the normal state of the transcriptome and cell functions. Genetic models like gene knockout (KO) are valuable for studying Tut4's functions.
In the context of coronavirus infection, depletion of host Tut4/7 leads to increased replication capacity of the mouse hepatitis virus (MHV), indicating that Tut4/7-mediated uridylation of MHV subgenomic RNAs marks them for decay and delays viral replication [1]. In miRNA and mRNA regulation, disruption of Tut4/7 expression in cells increases the abundance of various miRNAs, especially the let-7 family, and affects the levels of many of their mRNA targets. The mRNAs coding for proteins involved in cellular stress response, rRNA processing, etc., are most affected in Tut4/7 knockout cells [2]. Also, in cancer cell lines, mutation of Tut4/7 significantly reduces miRNA uridylation, causing defects in cancer cell properties like proliferation and migration, and leading to deregulation of miRNA-mRNA networks in a cell-type-specific manner [4].
In summary, Tut4 is essential for regulating RNA stability and abundance through uridylation, impacting multiple biological processes. Studies using KO models have revealed its roles in viral replication, miRNA/mRNA homeostasis, and cancer-related cell functions, providing insights into potential therapeutic strategies for viral diseases and cancer.
References:
1. Gupta, Ankit, Li, Yin, Chen, Shih-Heng, Martin, Negin P, Morgan, Marcos. 2023. TUT4/7-mediated uridylation of a coronavirus subgenomic RNAs delays viral replication. In Communications biology, 6, 438. doi:10.1038/s42003-023-04814-1. https://pubmed.ncbi.nlm.nih.gov/37085578/
2. Zhang, Pengcheng, Frederick, Mallory I, Heinemann, Ilka U. 2022. Terminal Uridylyltransferases TUT4/7 Regulate microRNA and mRNA Homeostasis. In Cells, 11, . doi:10.3390/cells11233742. https://pubmed.ncbi.nlm.nih.gov/36497000/
3. Yang, Acong, Bofill-De Ros, Xavier, Stanton, Ryan, Villanueva, Patricia, Gu, Shuo. 2022. TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and abundance. In Nature communications, 13, 5260. doi:10.1038/s41467-022-32969-8. https://pubmed.ncbi.nlm.nih.gov/36071058/
4. Medhi, Ragini, Price, Jonathan, Furlan, Giulia, Sapetschnig, Alexandra, Miska, Eric A. 2021. RNA uridyl transferases TUT4/7 differentially regulate miRNA variants depending on the cancer cell type. In RNA (New York, N.Y.), 28, 353-370. doi:10.1261/rna.078976.121. https://pubmed.ncbi.nlm.nih.gov/34949722/
5. Stein, Ross L, Wilson, David M. 2022. Kinetic and Mechanistic Studies of the Terminal Uridylyltransferase, Zcchc11 (TUT4). In Biochemistry, 61, 1614-1624. doi:10.1021/acs.biochem.2c00146. https://pubmed.ncbi.nlm.nih.gov/35797480/
6. Lim, Jaechul, Ha, Minju, Chang, Hyeshik, Patel, Dinshaw J, Kim, V Narry. . Uridylation by TUT4 and TUT7 marks mRNA for degradation. In Cell, 159, 1365-76. doi:10.1016/j.cell.2014.10.055. https://pubmed.ncbi.nlm.nih.gov/25480299/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen