C57BL/6JCya-Pabpc4em1flox/Cya
Common Name:
Pabpc4-flox
Product ID:
S-CKO-07364
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pabpc4-flox
Strain ID
CKOCMP-230721-Pabpc4-B6J-VA
Gene Name
Product ID
S-CKO-07364
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pabpc4em1flox/Cya mice (Catalog S-CKO-07364) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000080178
NCBI RefSeq
NM_130881
Target Region
Exon 2~3
Size of Effective Region
~3.8 kb
Detailed Document
Overview of Gene Research
Pabpc4, a cytoplasmic poly(A)-binding protein isoform, is an RNA-processing protein. It plays a vital role in enhancing translation and mRNA stability, thus promoting gene expression. It is involved in various biological processes such as erythroid differentiation and metabolic stress response, and is associated with pathways like selective autophagy, which is important for host-defense against virus invasion [1,3,4]. Genetic models like gene knockout could potentially be used to further study its functions.
In terms of its antiviral function, Pabpc4 can be regulated by transcription factor SP1 and broadly inhibits the replication of coronaviruses from four genera of the Coronaviridae family. It targets the nucleocapsid (N) protein of these coronaviruses, recruits the E3 ubiquitin ligase MARCH8/MARCHF8 for ubiquitination of the N protein. The ubiquitinated N protein is then recognized by the cargo receptor NDP52/CALCOCO2 and delivered to autolysosomes for degradation, impairing viral proliferation [1]. In addition, Pabpc4 knockdown experiments in 3T3-L1 pre-adipocytes suggest its role in regulating differentiation and energy metabolism in these cells [2]. Silencing of Pabpc4 in C2C12 and MEF cells induces an oxidative phenotype, through increasing the ubiquitination and degradation of NCoR1, leading to the derepression of PPAR-regulated genes, and enhancing lipid metabolism [3].
In conclusion, Pabpc4 is essential for gene expression regulation, and its functions extend to antiviral defense, adipocyte differentiation, and metabolic stress response. Research using knockout models could further clarify its roles, especially in relation to coronavirus-related diseases, type 2 diabetes, and metabolic diseases [1-3].
References:
1. Jiao, Yajuan, Kong, Ning, Wang, Hua, Tong, Guangzhi, Shan, Tongling. 2021. PABPC4 Broadly Inhibits Coronavirus Replication by Degrading Nucleocapsid Protein through Selective Autophagy. In Microbiology spectrum, 9, e0090821. doi:10.1128/Spectrum.00908-21. https://pubmed.ncbi.nlm.nih.gov/34612687/
2. Tang, Haibo, Wang, Jie, Deng, Peizhi, Zhu, Shaihong, Lu, Yao. 2023. Transcriptome-wide association study-derived genes as potential visceral adipose tissue-specific targets for type 2 diabetes. In Diabetologia, 66, 2087-2100. doi:10.1007/s00125-023-05978-5. https://pubmed.ncbi.nlm.nih.gov/37540242/
3. Oliveira, A G, Oliveira, L D, Cruz, M V, Auwerx, J, Silveira, L R. 2023. Interaction between poly(A)-binding protein PABPC4 and nuclear receptor corepressor NCoR1 modulates a metabolic stress response. In The Journal of biological chemistry, 299, 104702. doi:10.1016/j.jbc.2023.104702. https://pubmed.ncbi.nlm.nih.gov/37059182/
4. Kini, Hemant K, Kong, Jian, Liebhaber, Stephen A. 2014. Cytoplasmic poly(A) binding protein C4 serves a critical role in erythroid differentiation. In Molecular and cellular biology, 34, 1300-9. doi:10.1128/MCB.01683-13. https://pubmed.ncbi.nlm.nih.gov/24469397/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen