C57BL/6NCya-Zc3h12aem1flox/Cya
Common Name:
Zc3h12a-flox
Product ID:
S-CKO-07368
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Zc3h12a-flox
Strain ID
CKOCMP-230738-Zc3h12a-B6N-VA
Gene Name
Product ID
S-CKO-07368
Gene Alias
MCPIP; MCPIP-1; Mcpip1; Reg1
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Zc3h12aem1flox/Cya mice (Catalog S-CKO-07368) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000036188
NCBI RefSeq
NM_153159
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Zc3h12a, also known as MCPIP1 and Regnase-1, is the founding member of a ribonuclease family. It is an extensively studied regulator of the inflammatory response, acting as an RNase to degrade chemokine transcripts like IL-1 and its own mRNA, and as a transcription factor to reduce the expression of other chemokines induced by NF-kβ such as MCP-1. It also up-regulates genes involved in differentiation and apoptosis, playing a crucial role in maintaining an equilibrium in the immune response [1,2].
In the KC-Tie2 inflammatory dermatitis mouse model, siRNA knockdown of TCF4 led to increased Zc3h12a expression, creating a self-sustaining inflammatory feedback cycle. Zc3h12a knockdown decreased the expression of pro-inflammatory genes, revealing its pro-inflammatory role in skin inflammation [3]. In small cell lung cancer cells, knockdown of Zc3h12a impaired DNA repair ability, as seen by decreased BRCA1 expression and increased γH2AX at DNA damage sites, and sensitized the cells to radiotherapy [4]. Macrophage-specific Mcpip1 (Zc3h12a) deficiency in mice led to a more severe DSS-induced colitis, with increased macrophage migratory capacity and M1 polarisation but decreased monocyte-to-macrophage maturation, highlighting its role in mucosal inflammation [5].
In conclusion, Zc3h12a plays essential roles in inflammation-related biological processes, as revealed through model-based research. Its functions in maintaining immune equilibrium, regulating skin and mucosal inflammation, and affecting DNA damage repair in small cell lung cancer are significant. Mouse models, including KO/CKO models, have been crucial in uncovering these roles, providing insights into diseases such as inflammatory skin diseases, small cell lung cancer, and inflammatory bowel disease.
References:
1. Habacher, Cornelia, Ciosk, Rafal. 2017. ZC3H12A/MCPIP1/Regnase-1-related endonucleases: An evolutionary perspective on molecular mechanisms and biological functions. In BioEssays : news and reviews in molecular, cellular and developmental biology, 39, . doi:10.1002/bies.201700051. https://pubmed.ncbi.nlm.nih.gov/28719000/
2. Cifuentes, Ricardo A, Cruz-Tapias, Paola, Rojas-Villarraga, Adriana, Anaya, Juan-Manuel. 2010. ZC3H12A (MCPIP1): molecular characteristics and clinical implications. In Clinica chimica acta; international journal of clinical chemistry, 411, 1862-8. doi:10.1016/j.cca.2010.08.033. https://pubmed.ncbi.nlm.nih.gov/20807520/
3. Jiang, Yanyun, Gruszka, Dennis, Zeng, Chang, Gudjonsson, Johann E, Ward, Nicole L. 2024. Suppression of TCF4 promotes a ZC3H12A-mediated self-sustaining inflammatory feedback cycle involving IL-17RA/IL-17RE epidermal signaling. In JCI insight, 9, . doi:10.1172/jci.insight.172764. https://pubmed.ncbi.nlm.nih.gov/38470486/
4. Lu, Mingjun, Gao, Qing, Jin, Renjing, Wang, Jinghui, Ma, Teng. 2024. The Ribonuclease ZC3H12A is required for self-inflicted DNA breaks after DNA damage in small cell lung cancer cells. In Cellular oncology (Dordrecht, Netherlands), 47, 1497-1502. doi:10.1007/s13402-024-00941-x. https://pubmed.ncbi.nlm.nih.gov/38498096/
5. Lu, Huiying, Zhang, Cui, Wu, Wei, Kolattukudy, Pappachan E, Liu, Zhanju. 2022. MCPIP1 restrains mucosal inflammation by orchestrating the intestinal monocyte to macrophage maturation via an ATF3-AP1S2 axis. In Gut, 72, 882-895. doi:10.1136/gutjnl-2022-327183. https://pubmed.ncbi.nlm.nih.gov/37015751/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen