C57BL/6JCya-Kmt2cem1flox/Cya
Common Name:
Kmt2c-flox
Product ID:
S-CKO-07424
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Kmt2c-flox
Strain ID
CKOCMP-231051-Kmt2c-B6J-VA
Gene Name
Product ID
S-CKO-07424
Gene Alias
E330008K23Rik; HALR; Mll3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kmt2cem1flox/Cya mice (Catalog S-CKO-07424) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000045291
NCBI RefSeq
NM_001081383
Target Region
Exon 3
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
KMT2C, also known as MLL3, is a histone lysine methyltransferase responsible for the monomethylation of histone 3 lysine 4 (H3K4) residues at gene enhancer sites. It is part of the KMT2C/D COMPASS complexes, which regulate DNA promoter and enhancer elements, modulating gene expression in various cell types crucial for embryonic development, central nervous system development, and post-natal survival [3]. It is involved in multiple signaling pathways such as EMT, p53, Myc, and DNA damage repair [5].
In SCLC mouse models, KMT2C deficiency promoted multiple-organ metastases. Mechanistically, KMT2C regulated DNMT3A expression through histone methylation, and forced DNMT3A expression restrained metastasis [1]. In non-metastatic murine models of TNBC, deletion of Kmt2c drove metastasis, especially to the brain, via KDM6A-dependent upregulation of MMP3 [2]. KMT2C knockout in cortical neurons and the mouse brain led to ASD-like behaviors [4]. In a Pten-deficient PCa mouse model, deletion of the Kmt2c SET domain drove proliferation, PIN formation, and metastatic dissemination [6].
In conclusion, KMT2C plays a crucial role in various biological processes through its histone methylation function. Gene knockout mouse models have revealed its significance in cancer metastasis, such as in SCLC, TNBC, and prostate cancer, as well as in neurodevelopmental disorders like ASD. These findings contribute to understanding the underlying mechanisms of these diseases and may provide potential therapeutic targets.
References:
1. Na, Feifei, Pan, Xiangyu, Chen, Jingyao, Wei, Yuquan, Chen, Chong. 2022. KMT2C deficiency promotes small cell lung cancer metastasis through DNMT3A-mediated epigenetic reprogramming. In Nature cancer, 3, 753-767. doi:10.1038/s43018-022-00361-6. https://pubmed.ncbi.nlm.nih.gov/35449309/
2. Seehawer, Marco, Li, Zheqi, Nishida, Jun, Papanastasiou, Malvina, Polyak, Kornelia. 2024. Loss of Kmt2c or Kmt2d drives brain metastasis via KDM6A-dependent upregulation of MMP3. In Nature cell biology, 26, 1165-1175. doi:10.1038/s41556-024-01446-3. https://pubmed.ncbi.nlm.nih.gov/38926506/
3. Lavery, William J, Barski, Artem, Wiley, Susan, Schorry, Elizabeth K, Lindsley, Andrew W. 2020. KMT2C/D COMPASS complex-associated diseases [KCDCOM-ADs]: an emerging class of congenital regulopathies. In Clinical epigenetics, 12, 10. doi:10.1186/s13148-019-0802-2. https://pubmed.ncbi.nlm.nih.gov/31924266/
4. Brauer, Bastian, Merino-Veliz, Nicolas, Ahumada-Marchant, Constanza, Arriagada, Gloria, Bustos, Fernando J. 2023. KMT2C knockout generates ASD-like behaviors in mice. In Frontiers in cell and developmental biology, 11, 1227723. doi:10.3389/fcell.2023.1227723. https://pubmed.ncbi.nlm.nih.gov/37538398/
5. Jiao, Yunjuan, Lv, Yuanhao, Liu, Mingjie, Kang, Xiaohong, Su, Wei. 2024. The modification role and tumor association with a methyltransferase: KMT2C. In Frontiers in immunology, 15, 1444923. doi:10.3389/fimmu.2024.1444923. https://pubmed.ncbi.nlm.nih.gov/39165358/
6. Limberger, Tanja, Schlederer, Michaela, Trachtová, Karolina, Lagger, Sabine, Kenner, Lukas. 2022. KMT2C methyltransferase domain regulated INK4A expression suppresses prostate cancer metastasis. In Molecular cancer, 21, 89. doi:10.1186/s12943-022-01542-8. https://pubmed.ncbi.nlm.nih.gov/35354467/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen