C57BL/6JCya-Gckrem1flox/Cya
Common Name:
Gckr-flox
Product ID:
S-CKO-07429
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gckr-flox
Strain ID
CKOCMP-231103-Gckr-B6J-VA
Gene Name
Product ID
S-CKO-07429
Gene Alias
GKRP
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gckrem1flox/Cya mice (Catalog S-CKO-07429) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000072228
NCBI RefSeq
NM_144909
Target Region
Exon 4~8
Size of Effective Region
~3.8 kb
Detailed Document
Overview of Gene Research
Gckr, which encodes GKRP, is a key regulator of hepatic glucokinase (GCK). It influences the hepatic cytosolic NADH/NAD+ ratio (referred to as reductive stress), and this stress is linked to the activation of the transcription factor ChREBP, highlighting its importance in metabolic pathways [1].
Common genetic variants in Gckr have pleiotropic effects on multiple metabolic traits. For instance, these variants are associated with an increased risk of non-alcoholic fatty liver disease (NAFLD), gestational diabetes mellitus (GDM), and the transition from asymptomatic hyperuricaemia to gout. In Chinese patients with type 2 diabetes, the Gckr rs1260326 T allele carriers are at a high risk for end-stage kidney disease [2,3,4,5,6].
In conclusion, Gckr is crucial in regulating metabolic processes through its influence on reductive stress and associated transcription factors. The study of Gckr genetic variants in various disease conditions, such as NAFLD, GDM, gout, and kidney disease in diabetes, helps in understanding disease mechanisms and potentially identifying high-risk patients for early intervention.
References：
1. Singh, Charandeep, Jin, Byungchang, Shrestha, Nirajan, Mootha, Vamsi K, Goodman, Russell P. 2023. ChREBP is activated by reductive stress and mediates GCKR-associated metabolic traits. In Cell metabolism, 36, 144-158.e7. doi:10.1016/j.cmet.2023.11.010. https://pubmed.ncbi.nlm.nih.gov/38101397/
2. Ioannou, George N. 2021. Epidemiology and risk-stratification of NAFLD-associated HCC. In Journal of hepatology, 75, 1476-1484. doi:10.1016/j.jhep.2021.08.012. https://pubmed.ncbi.nlm.nih.gov/34453963/
3. Zhu, Manning, Lv, Yaer, Peng, Yanqing, Tian, Jiawei, Sun, Litao. 2023. GCKR and ADIPOQ gene polymorphisms in women with gestational diabetes mellitus. In Acta diabetologica, 60, 1709-1718. doi:10.1007/s00592-023-02165-1. https://pubmed.ncbi.nlm.nih.gov/37524927/
4. Li, Jiaying, Zhao, Yuening, Zhang, Hongxiang, Yang, Xiaoqin, Zhu, Liyan. . Contribution of Rs780094 and Rs1260326 Polymorphisms in GCKR Gene to Non-alcoholic Fatty Liver Disease: A Meta-Analysis Involving 26,552 Participants. In Endocrine, metabolic & immune disorders drug targets, 21, 1696-1708. doi:10.2174/1871530320999201126202706. https://pubmed.ncbi.nlm.nih.gov/33243135/
5. Sandoval-Plata, Gabriela, Morgan, Kevin, Abhishek, Abhishek. 2021. Variants in urate transporters, ADH1B, GCKR and MEPE genes associate with transition from asymptomatic hyperuricaemia to gout: results of the first gout versus asymptomatic hyperuricaemia GWAS in Caucasians using data from the UK Biobank. In Annals of the rheumatic diseases, 80, 1220-1226. doi:10.1136/annrheumdis-2020-219796. https://pubmed.ncbi.nlm.nih.gov/33832965/
6. Wang, Ke, Shi, Mai, Yang, Aimin, Chan, Juliana C N, Chow, Elaine. 2022. GCKR and GCK polymorphisms are associated with increased risk of end-stage kidney disease in Chinese patients with type 2 diabetes: The Hong Kong Diabetes Register (1995-2019). In Diabetes research and clinical practice, 193, 110118. doi:10.1016/j.diabres.2022.110118. https://pubmed.ncbi.nlm.nih.gov/36243233/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen