C57BL/6JCya-Tada2bem1flox/Cya
Common Name:
Tada2b-flox
Product ID:
S-CKO-07436
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tada2b-flox
Strain ID
CKOCMP-231151-Tada2b-B6J-VA
Gene Name
Product ID
S-CKO-07436
Gene Alias
Gm145
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tada2bem1flox/Cya mice (Catalog S-CKO-07436) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031097
NCBI RefSeq
NM_001170454
Target Region
Exon 2
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Tada2b, also known as Ada2beta, is a subunit of the chromatin-modifying complex SAGA. It plays central roles in regulating pluripotency, survival, growth, and lineage specification in human embryonic stem cells [3]. It is involved in histone acetylation pathways as part of the SAGA complex, which impacts gene expression and is crucial for normal cellular function and development.
In a melanoma model, genome-scale CRISPR-Cas9 knockout screening identified Tada2b as a gene whose loss is involved in resistance to vemurafenib, a therapeutic RAF inhibitor [1]. Protein-Protein Interaction analysis indicated that Tada2b levels were negatively correlated with KAT2A expression [2]. In an exploratory study on MDD, increased expression of Tada2b was found in hyperphagic MDD, with significant enrichment of gene ontology pathways mainly related to inflammation [4].
In conclusion, Tada2b is a key regulator within the SAGA complex, influencing important cellular processes such as pluripotency and survival. Its role in melanoma drug resistance and association with MDD-related inflammation suggest its potential significance in cancer treatment and understanding mood disorders. The use of gene-editing techniques like CRISPR-Cas9 in knockout screens has been valuable in uncovering these functions.
References：
1. Shalem, Ophir, Sanjana, Neville E, Hartenian, Ella, Doench, John G, Zhang, Feng. 2013. Genome-scale CRISPR-Cas9 knockout screening in human cells. In Science (New York, N.Y.), 343, 84-87. doi:10.1126/science.1247005. https://pubmed.ncbi.nlm.nih.gov/24336571/
2. Li, Hua, Li, Chun, Yang, Lu-Zong, Liu, Ji. . Integrative analysis of histone acetyltransferase KAT2A in human cancer. In Cancer biomarkers : section A of Disease markers, 38, 443-463. doi:10.3233/CBM-220464. https://pubmed.ncbi.nlm.nih.gov/38007639/
3. Naxerova, Kamila, Di Stefano, Bruno, Makofske, Jessica L, Hochedlinger, Konrad, Elledge, Stephen J. 2021. Integrated loss- and gain-of-function screens define a core network governing human embryonic stem cell behavior. In Genes & development, 35, 1527-1547. doi:10.1101/gad.349048.121. https://pubmed.ncbi.nlm.nih.gov/34711655/
4. Dan, Shu, Hall, Julia R, Holsen, Laura M, Klengel, Torsten. 2024. Divergent transcriptomic profiles in depressed individuals with hyper- and hypophagia implicating inflammatory status. In Journal of psychiatric research, 179, 209-219. doi:10.1016/j.jpsychires.2024.09.020. https://pubmed.ncbi.nlm.nih.gov/39316935/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen