C57BL/6JCya-Tada2bem1flox/Cya
Common Name
Tada2b-flox
Product ID
S-CKO-07436
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-231151-Tada2b-B6J-VA
When using this mouse strain in a publication, please cite “Tada2b-flox Mouse (Catalog S-CKO-07436) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tada2b-flox
Strain ID
CKOCMP-231151-Tada2b-B6J-VA
Gene Name
Product ID
S-CKO-07436
Gene Alias
Gm145
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000031097
NCBI RefSeq
NM_001170454
Target Region
Exon 2
Size of Effective Region
~1.8 kb
Overview of Gene Research
Tada2b, also known as Ada2beta, is a subunit of the chromatin-modifying complex SAGA. It plays central roles in regulating pluripotency, survival, growth, and lineage specification in human embryonic stem cells [3]. It is involved in histone acetylation pathways as part of the SAGA complex, which impacts gene expression and is crucial for normal cellular function and development.
In a melanoma model, genome-scale CRISPR-Cas9 knockout screening identified Tada2b as a gene whose loss is involved in resistance to vemurafenib, a therapeutic RAF inhibitor [1]. Protein-Protein Interaction analysis indicated that Tada2b levels were negatively correlated with KAT2A expression [2]. In an exploratory study on MDD, increased expression of Tada2b was found in hyperphagic MDD, with significant enrichment of gene ontology pathways mainly related to inflammation [4].
In conclusion, Tada2b is a key regulator within the SAGA complex, influencing important cellular processes such as pluripotency and survival. Its role in melanoma drug resistance and association with MDD-related inflammation suggest its potential significance in cancer treatment and understanding mood disorders. The use of gene-editing techniques like CRISPR-Cas9 in knockout screens has been valuable in uncovering these functions.
References：
1. Shalem, Ophir, Sanjana, Neville E, Hartenian, Ella, Doench, John G, Zhang, Feng. 2013. Genome-scale CRISPR-Cas9 knockout screening in human cells. In Science (New York, N.Y.), 343, 84-87. doi:10.1126/science.1247005. https://pubmed.ncbi.nlm.nih.gov/24336571/
2. Li, Hua, Li, Chun, Yang, Lu-Zong, Liu, Ji. . Integrative analysis of histone acetyltransferase KAT2A in human cancer. In Cancer biomarkers : section A of Disease markers, 38, 443-463. doi:10.3233/CBM-220464. https://pubmed.ncbi.nlm.nih.gov/38007639/
3. Naxerova, Kamila, Di Stefano, Bruno, Makofske, Jessica L, Hochedlinger, Konrad, Elledge, Stephen J. 2021. Integrated loss- and gain-of-function screens define a core network governing human embryonic stem cell behavior. In Genes & development, 35, 1527-1547. doi:10.1101/gad.349048.121. https://pubmed.ncbi.nlm.nih.gov/34711655/
4. Dan, Shu, Hall, Julia R, Holsen, Laura M, Klengel, Torsten. 2024. Divergent transcriptomic profiles in depressed individuals with hyper- and hypophagia implicating inflammatory status. In Journal of psychiatric research, 179, 209-219. doi:10.1016/j.jpsychires.2024.09.020. https://pubmed.ncbi.nlm.nih.gov/39316935/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.