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C57BL/6JCya-Lin54em1flox/Cya
Common Name:
Lin54-flox
Product ID:
S-CKO-07461
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lin54-flox
Strain ID
CKOCMP-231506-Lin54-B6J-VA
Gene Name
Lin54
Product ID
S-CKO-07461
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
231506
Modification
Conditional knockout
Chromosome
5
Phenotype
MGI:2140902
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lin54em1flox/Cya mice (Catalog S-CKO-07461) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000239513
NCBI RefSeq
NM_001115010
Target Region
Exon 6~7
Size of Effective Region
~1.4 kb
Detailed Document
Click here to download >>
Overview of Gene Research
LIN54 is an essential core subunit of the DREAM/LINC (LIN complex) and MuvB complexes. These complexes play crucial roles in the coordinated regulation of cell cycle genes, either activating transcription during S-G2 phases or repressing it during quiescence [1,3,4]. LIN54 is required for cell cycle progression and has a DNA-binding domain (CHC domain) which consists of two cysteine-rich (CXC) domains, enabling it to bind to the cdc2 promoter in a sequence-specific manner [1].

In Drosophila, the LIN54 homolog Mip120 controls two aspects of oogenesis. Its absence leads to egg chamber arrest during the transition from stage 7 to 8 due to failure in normal chromosomal dynamics in ovarian nurse cells, and also causes a dramatic increase in the expression of benign gonial cell neoplasm (bgcn) [2]. In mouse embryonic stem cells (ESCs), deficiency of Lin54 triggers G2/M arrest, loss of pluripotency, and spontaneous differentiation, and these abnormalities can be partially rescued by ectopic co-expression of Cyclin B1 and Cdk1 [5].

In conclusion, LIN54 is vital for cell cycle regulation and has a significant impact on processes such as oogenesis and maintaining embryonic stem cell identity. The study of LIN54 through model-based research, like in Drosophila and mouse ESCs, provides insights into its role in normal development and potentially in disease conditions related to cell cycle dysregulation [2,5].

References:
1. Schmit, Fabienne, Cremer, Sarah, Gaubatz, Stefan. 2009. LIN54 is an essential core subunit of the DREAM/LINC complex that binds to the cdc2 promoter in a sequence-specific manner. In The FEBS journal, 276, 5703-16. doi:10.1111/j.1742-4658.2009.07261.x. https://pubmed.ncbi.nlm.nih.gov/19725879/
2. Cheng, Mei-Hsin, Andrejka, Laura, Vorster, Paul J, Hinman, Albert, Lipsick, Joseph S. 2017. The Drosophila LIN54 homolog Mip120 controls two aspects of oogenesis. In Biology open, 6, 967-978. doi:10.1242/bio.025825. https://pubmed.ncbi.nlm.nih.gov/28522430/
3. Iness, Audra N, Litovchick, Larisa. 2018. MuvB: A Key to Cell Cycle Control in Ovarian Cancer. In Frontiers in oncology, 8, 223. doi:10.3389/fonc.2018.00223. https://pubmed.ncbi.nlm.nih.gov/29942794/
4. Walston, Hayley, Iness, Audra N, Litovchick, Larisa. 2021. DREAM On: Cell Cycle Control in Development and Disease. In Annual review of genetics, 55, 309-329. doi:10.1146/annurev-genet-071819-103836. https://pubmed.ncbi.nlm.nih.gov/34496610/
5. Wang, Congcong, Hao, Kunying, Dong, Lixia, Jiang, Qing, Qin, Jinzhong. 2022. The MuvB complex safeguards embryonic stem cell identity through regulation of the cell cycle machinery. In The Journal of biological chemistry, 298, 101701. doi:10.1016/j.jbc.2022.101701. https://pubmed.ncbi.nlm.nih.gov/35148988/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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