C57BL/6JCya-Gpat3em1flox/Cya
Common Name:
Gpat3-flox
Product ID:
S-CKO-07463
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Gpat3-flox
Strain ID
CKOCMP-231510-Gpat3-B6J-VA
Gene Name
Product ID
S-CKO-07463
Gene Alias
1-AGPAT; 1-AGPAT 9; 4933407I02Rik; 4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3; mGPAT3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpat3em1flox/Cya mice (Catalog S-CKO-07463) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031255
NCBI RefSeq
NM_172715
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Gpat3, or glycerol-3-phosphate acyltransferase 3, is a key rate-limiting enzyme regulating triglyceride synthesis [1]. It is involved in lipid metabolic pathways and plays important roles in various biological processes, such as adipogenesis [4]. Genetic models, like gene knockout mouse models, are valuable for studying its functions.
In mouse models, GPAT3 deficiency attenuates corticosterone-caused hepatic steatosis and oxidative stress through the GSK3β/Nrf2 signals [1]. In Seipin -/-Gpat3 -/- mice, there is a significant improvement in liver steatosis and insulin sensitivity, suggesting a functional link between seipin and GPAT3 [3]. In addition, GPAT3 -/- mice show reduced Kupffer cell inflammation reaction, accompanied by improved mitochondrial function and decreased production of lysophosphatidic acid (LPA) [2].
In conclusion, GPAT3 is crucial in lipid metabolism, especially in triglyceride synthesis. Studies using KO mouse models have revealed its roles in diseases like hepatic steatosis, insulin resistance, and inflammation-related liver diseases, providing insights into potential therapeutic strategies targeting GPAT3 for these conditions.
References:
1. Fan, Guoqiang, Huang, Lingling, Wang, Mengxuan, Li, Yanfei, Yang, Xiaojing. 2024. GPAT3 deficiency attenuates corticosterone-caused hepatic steatosis and oxidative stress through GSK3β/Nrf2 signals. In Biochimica et biophysica acta. Molecular basis of disease, 1870, 167007. doi:10.1016/j.bbadis.2023.167007. https://pubmed.ncbi.nlm.nih.gov/38185063/
2. Fan, Guoqiang, Li, Yanfei, Zong, Yibo, Gao, Mingming, Yang, Xiaojing. 2023. GPAT3 regulates the synthesis of lipid intermediate LPA and exacerbates Kupffer cell inflammation mediated by the ERK signaling pathway. In Cell death & disease, 14, 208. doi:10.1038/s41419-023-05741-z. https://pubmed.ncbi.nlm.nih.gov/36964139/
3. Gao, Mingming, Liu, Lin, Wang, Xiaowei, Liu, George, Yang, Hongyuan. . GPAT3 deficiency alleviates insulin resistance and hepatic steatosis in a mouse model of severe congenital generalized lipodystrophy. In Human molecular genetics, 29, 432-443. doi:10.1093/hmg/ddz300. https://pubmed.ncbi.nlm.nih.gov/31873720/
4. Shan, Dandan, Li, Jian-liang, Wu, Leeying, Gimeno, Ruth E, Cao, Jingsong. 2010. GPAT3 and GPAT4 are regulated by insulin-stimulated phosphorylation and play distinct roles in adipogenesis. In Journal of lipid research, 51, 1971-81. doi:10.1194/jlr.M006304. https://pubmed.ncbi.nlm.nih.gov/20181984/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen