C57BL/6JCya-Daglbem1flox/Cya
Common Name:
Daglb-flox
Product ID:
S-CKO-07504
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Daglb-flox
Strain ID
CKOCMP-231871-Daglb-B6J-VA
Gene Name
Product ID
S-CKO-07504
Gene Alias
E330036I19Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Daglbem1flox/Cya mice (Catalog S-CKO-07504) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000045593
NCBI RefSeq
NM_144915
Target Region
Exon 2
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Daglb, or diacylglycerol lipase β, is the main synthase responsible for generating 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in the brain. The endocannabinoid system, in which Daglb-mediated 2-AG biosynthesis is a key part, is involved in regulating diverse neural functions. It has implications in multiple biological processes and diseases, making it an important gene for study, especially with the help of genetic models [1,2].
Genetic knockdown of Daglb in nigral dopaminergic neurons (DANs) of mice substantially reduced substantia nigra (SN) 2-AG levels and impaired locomotor skill learning, particularly across-session learning. This indicates that DAGLB-deficiency contributes to the pathogenesis of Parkinsonism, highlighting the importance of DAGLB-mediated 2-AG biosynthesis in nigral DANs in regulating neuronal activity and dopamine release [1]. In addition, in AP-4-deficient neurons (where AP-4 is involved in anterograde axonal transport), DAGLB accumulates at the trans-Golgi network, axonal DAGLB levels are reduced, and 2-AG levels in the brains of AP-4 knockout mice are decreased. Neurite growth defects of these neurons can be rescued by inhibiting the enzyme responsible for 2-AG hydrolysis, suggesting a role for DAGLB-mediated 2-AG signaling in axon growth [2].
In conclusion, Daglb is crucial for endocannabinoid synthesis, especially in the brain. Studies using gene-knockout mouse models have revealed its significant role in Parkinsonism pathogenesis and axon growth. Understanding Daglb's functions provides insights into neural function regulation and potential therapeutic targets for related neurological disorders.
References:
1. Liu, Zhenhua, Yang, Nannan, Dong, Jie, Cai, Huaibin, Tang, Beisha. 2022. Deficiency in endocannabinoid synthase DAGLB contributes to early onset Parkinsonism and murine nigral dopaminergic neuron dysfunction. In Nature communications, 13, 3490. doi:10.1038/s41467-022-31168-9. https://pubmed.ncbi.nlm.nih.gov/35715418/
2. Davies, Alexandra K, Alecu, Julian E, Ziegler, Marvin, Ebrahimi-Fakhari, Darius, Borner, Georg H H. 2022. AP-4-mediated axonal transport controls endocannabinoid production in neurons. In Nature communications, 13, 1058. doi:10.1038/s41467-022-28609-w. https://pubmed.ncbi.nlm.nih.gov/35217685/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen