C57BL/6JCya-Gimap6em1flox/Cya
Common Name:
Gimap6-flox
Product ID:
S-CKO-07511
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gimap6-flox
Strain ID
CKOCMP-231931-Gimap6-B6J-VA
Gene Name
Product ID
S-CKO-07511
Gene Alias
4833419H03Rik; Ian6; mFLJ00102
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gimap6em1flox/Cya mice (Catalog S-CKO-07511) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000053661
NCBI RefSeq
NM_153175
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
GIMAP6, the GTPase of the immune-associated protein 6, is a gene crucial for autophagy, immune competence, and inflammation. It belongs to the GIMAP GTPases family, which is strongly expressed in the adaptive immune system. GIMAP6 is involved in pathways such as redox regulation, and its encoded protein may play a role in T-cell receptor signaling pathway, chemokine signaling pathway, and cytokine-cytokine receptor interaction [1,3].
In Gimap6-/-mice, there are defects in autophagy, redox regulation, and polyunsaturated fatty acid-containing lipids. Mice with deletion of Gimap6 within the T and B cell lineages show a 50-70% reduction in peripheral CD4+ and CD8+ T cells, along with autophagic disruption as indicated by increased levels of MAP1LC3B lipidated form and S405-phosphorylation of SQSTM1, and increased mitochondrial/cytoplasmic volume ratio and autophagosome numbers [1,2]. Gimap6-/-mice also die prematurely from microangiopathic glomerulosclerosis likely due to GIMAP6 deficiency in kidney endothelial cells [1]. In human patients with mutations in GIMAP6, there are infections, lymphoproliferation, autoimmunity, and multiorgan vasculitis, along with accelerated apoptosis [1,4].
In conclusion, GIMAP6 is essential for normal autophagic processes and the maintenance of a normal peripheral adaptive immune system. The study of Gimap6 knockout mouse models has revealed its crucial role in immune-related diseases, such as infections, autoimmunity, and multiorgan vasculitis, as well as in kidney-related pathologies like microangiopathic glomerulosclerosis [1,2,4].
References:
1. Yao, Yikun, Du Jiang, Ping, Chao, Brittany N, Simon, Anna Katharina, Lenardo, Michael J. 2022. GIMAP6 regulates autophagy, immune competence, and inflammation in mice and humans. In The Journal of experimental medicine, 219, . doi:10.1084/jem.20201405. https://pubmed.ncbi.nlm.nih.gov/35551368/
2. Pascall, John C, Webb, Louise M C, Eskelinen, Eeva-Liisa, Attaf-Bouabdallah, Noudjoud, Butcher, Geoffrey W. 2018. GIMAP6 is required for T cell maintenance and efficient autophagy in mice. In PloS one, 13, e0196504. doi:10.1371/journal.pone.0196504. https://pubmed.ncbi.nlm.nih.gov/29718959/
3. Chen, Xiuqiong, Li, Zhaona, Wang, Xinyue, Chen, Kaidi, Jiang, Richeng. 2023. Investigation and verification of GIMAP6 as a robust biomarker for prognosis and tumor immunity in lung adenocarcinoma. In Journal of cancer research and clinical oncology, 149, 11041-11055. doi:10.1007/s00432-023-04980-z. https://pubmed.ncbi.nlm.nih.gov/37338641/
4. Shadur, Bella, Asherie, Nathalie, Kfir-Erenfeld, Shlomit, Mor-Shaked, Hagar, Stepensky, Polina. 2020. A human case of GIMAP6 deficiency: a novel primary immune deficiency. In European journal of human genetics : EJHG, 29, 657-662. doi:10.1038/s41431-020-00773-x. https://pubmed.ncbi.nlm.nih.gov/33328581/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen