Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Ccdc174em1flox/Cya
Common Name:
Ccdc174-flox
Product ID:
S-CKO-07537
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ccdc174-flox
Strain ID
CKOCMP-232236-Ccdc174-B6J-VA
Gene Name
Ccdc174
Product ID
S-CKO-07537
Gene Alias
C130022K22Rik
Background
C57BL/6JCya
NCBI ID
232236
Modification
Conditional knockout
Chromosome
6
Phenotype
MGI:2444652
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccdc174em1flox/Cya mice (Catalog S-CKO-07537) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000037783
NCBI RefSeq
NM_172730
Target Region
Exon 2~3
Size of Effective Region
~1.7 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Ccdc174, short for coiled-coil domain-containing 174, is involved in multiple biological processes. It functions as an RNA-binding protein in mouse ES cells, associating with U1 snRNA and 5' splice sites, and is required for the selection and effective processing of weak 5' splice sites during pre-mRNA splicing, which is crucial for proper gene expression [2]. It has also been associated with mammalian reproduction and immunity-related biological processes [4,5].

In bulls, the expression level of Ccdc174 was significantly up-regulated in high-fertile bulls compared to sub-fertile ones. A fertility prediction multivariate model including Ccdc174 had a high coefficient of determination with the field conception rate, suggesting it can be a potential marker for assessing bull fertility [1]. In humans, a homozygous mutation in Ccdc174 (c.1404A>G, p.[*468Trpext*6]) was found in individuals with a syndrome of hypotonia and psychomotor developmental delay. Knockdown of the Ccdc174 ortholog in Xenopus laevis embryos led to poor neural fold closure, embryonic lethality, and reduction in the expression of neural markers. Overexpression of the mutant Ccdc174 in neuroblastoma cells caused apoptosis, and the mutation also led to myopathic changes in skeletal muscle [3].

In conclusion, Ccdc174 is important for pre-mRNA splicing, semen quality in bulls, and normal development in vertebrates. Its abnormal mutation is associated with a syndrome of hypotonia and psychomotor developmental delay in humans. Research on Ccdc174 helps in understanding biological processes related to reproduction, development, and disease mechanisms.

References:
1. Selvaraju, Sellappan, Swathi, Divakar, Ramya, Laxman, Archana, Santhanahalli Siddalingappa, Sivaram, Muniandy. 2020. Orchestrating the expression levels of sperm mRNAs reveals CCDC174 as an important determinant of semen quality and bull fertility. In Systems biology in reproductive medicine, 67, 89-101. doi:10.1080/19396368.2020.1836286. https://pubmed.ncbi.nlm.nih.gov/33190538/
2. Flemr, Matyas, Schwaiger, Michaela, Hess, Daniel, Mohn, Fabio, Bühler, Marc. 2023. Mouse nuclear RNAi-defective 2 promotes splicing of weak 5' splice sites. In RNA (New York, N.Y.), 29, 1140-1165. doi:10.1261/rna.079465.122. https://pubmed.ncbi.nlm.nih.gov/37137667/
3. Volodarsky, Michael, Lichtig, Hava, Leibson, Tom, Frank, Dale, Birk, Ohad S. 2015. CDC174, a novel component of the exon junction complex whose mutation underlies a syndrome of hypotonia and psychomotor developmental delay. In Human molecular genetics, 24, 6485-91. doi:10.1093/hmg/ddv357. https://pubmed.ncbi.nlm.nih.gov/26358778/
4. Liu, Kai, Liu, Yufang, Chu, Mingxing. 2024. Detection of polymorphisms in six genes and their association analysis with litter size in sheep. In Animal biotechnology, 35, 2309954. doi:10.1080/10495398.2024.2309954. https://pubmed.ncbi.nlm.nih.gov/38294691/
5. Marete, Andrew, Ariel, Olivier, Ibeagha-Awemu, Eveline, Bissonnette, Nathalie. 2021. Identification of Long Non-coding RNA Isolated From Naturally Infected Macrophages and Associated With Bovine Johne's Disease in Canadian Holstein Using a Combination of Neural Networks and Logistic Regression. In Frontiers in veterinary science, 8, 639053. doi:10.3389/fvets.2021.639053. https://pubmed.ncbi.nlm.nih.gov/33969037/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest