C57BL/6JCya-Ccdc174em1flox/Cya
Common Name:
Ccdc174-flox
Product ID:
S-CKO-07537
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ccdc174-flox
Strain ID
CKOCMP-232236-Ccdc174-B6J-VA
Gene Name
Product ID
S-CKO-07537
Gene Alias
C130022K22Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccdc174em1flox/Cya mice (Catalog S-CKO-07537) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000037783
NCBI RefSeq
NM_172730
Target Region
Exon 2~3
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Ccdc174, short for coiled-coil domain-containing 174, is involved in multiple biological processes. It functions as an RNA-binding protein in mouse ES cells, associating with U1 snRNA and 5' splice sites, and is required for the selection and effective processing of weak 5' splice sites during pre-mRNA splicing, which is crucial for proper gene expression [2]. It has also been associated with mammalian reproduction and immunity-related biological processes [4,5].
In bulls, the expression level of Ccdc174 was significantly up-regulated in high-fertile bulls compared to sub-fertile ones. A fertility prediction multivariate model including Ccdc174 had a high coefficient of determination with the field conception rate, suggesting it can be a potential marker for assessing bull fertility [1]. In humans, a homozygous mutation in Ccdc174 (c.1404A>G, p.[*468Trpext*6]) was found in individuals with a syndrome of hypotonia and psychomotor developmental delay. Knockdown of the Ccdc174 ortholog in Xenopus laevis embryos led to poor neural fold closure, embryonic lethality, and reduction in the expression of neural markers. Overexpression of the mutant Ccdc174 in neuroblastoma cells caused apoptosis, and the mutation also led to myopathic changes in skeletal muscle [3].
In conclusion, Ccdc174 is important for pre-mRNA splicing, semen quality in bulls, and normal development in vertebrates. Its abnormal mutation is associated with a syndrome of hypotonia and psychomotor developmental delay in humans. Research on Ccdc174 helps in understanding biological processes related to reproduction, development, and disease mechanisms.
References:
1. Selvaraju, Sellappan, Swathi, Divakar, Ramya, Laxman, Archana, Santhanahalli Siddalingappa, Sivaram, Muniandy. 2020. Orchestrating the expression levels of sperm mRNAs reveals CCDC174 as an important determinant of semen quality and bull fertility. In Systems biology in reproductive medicine, 67, 89-101. doi:10.1080/19396368.2020.1836286. https://pubmed.ncbi.nlm.nih.gov/33190538/
2. Flemr, Matyas, Schwaiger, Michaela, Hess, Daniel, Mohn, Fabio, Bühler, Marc. 2023. Mouse nuclear RNAi-defective 2 promotes splicing of weak 5' splice sites. In RNA (New York, N.Y.), 29, 1140-1165. doi:10.1261/rna.079465.122. https://pubmed.ncbi.nlm.nih.gov/37137667/
3. Volodarsky, Michael, Lichtig, Hava, Leibson, Tom, Frank, Dale, Birk, Ohad S. 2015. CDC174, a novel component of the exon junction complex whose mutation underlies a syndrome of hypotonia and psychomotor developmental delay. In Human molecular genetics, 24, 6485-91. doi:10.1093/hmg/ddv357. https://pubmed.ncbi.nlm.nih.gov/26358778/
4. Liu, Kai, Liu, Yufang, Chu, Mingxing. 2024. Detection of polymorphisms in six genes and their association analysis with litter size in sheep. In Animal biotechnology, 35, 2309954. doi:10.1080/10495398.2024.2309954. https://pubmed.ncbi.nlm.nih.gov/38294691/
5. Marete, Andrew, Ariel, Olivier, Ibeagha-Awemu, Eveline, Bissonnette, Nathalie. 2021. Identification of Long Non-coding RNA Isolated From Naturally Infected Macrophages and Associated With Bovine Johne's Disease in Canadian Holstein Using a Combination of Neural Networks and Logistic Regression. In Frontiers in veterinary science, 8, 639053. doi:10.3389/fvets.2021.639053. https://pubmed.ncbi.nlm.nih.gov/33969037/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen