C57BL/6JCya-Ccdc106em1flox/Cya
Common Name:
Ccdc106-flox
Product ID:
S-CKO-07586
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ccdc106-flox
Strain ID
CKOCMP-232821-Ccdc106-B6J-VA
Gene Name
Product ID
S-CKO-07586
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccdc106em1flox/Cya mice (Catalog S-CKO-07586) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000108571
NCBI RefSeq
NM_001290429
Target Region
Exon 5~6
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
CCDC106, Coiled-coil domain-containing protein 106, is a protein-coding gene. It has been identified as a p53-interacting partner and is involved in regulating the p53-Mdm2/MdmX signaling axis. This regulation is crucial as overexpressed Mdm2/MdmX inhibits cellular p53 activity, which is highly relevant to cancer occurrence [1].
CCDC106 overexpression downregulates the cellular levels of p53 and Mdm2/MdmX, and decreased p53 reversibly downregulates the cellular level of CCDC106 [1]. In ovarian cancer, overexpression of CCDC106 promotes the proliferation, invasion, and Epithelial-to-mesenchymal transition (EMT) of mutant p53 cells through the ATF4-mediated inhibition of p21 [2]. In non-small cell lung cancer, CCDC106 expression is associated with advanced TNM stage, positive regional lymph node metastasis, and poor overall survival. It promotes cell proliferation via AKT-dependent upregulation of Cyclin A2 and Cyclin B1 [3]. In cervical cancer, HPV-CCDC106 integration alters local chromosome architecture, hijacks an enhancer, and increases CCDC106 expression, which then promotes cancer progression by degrading p53 [4,6]. Also, in breast and cervical cancers with wild-type p53, CK2-mediated phosphorylation of CCDC106 at Ser-130 and Ser-147 is required for its interaction with p53, nuclear localization, and subsequent p53 degradation and oncogenic function [5].
In conclusion, CCDC106 plays a significant role in cancer-related biological processes, mainly through its interaction with p53 and regulation of related signaling pathways. Functional studies, especially those using in vivo models, have revealed its oncogenic potential in multiple cancer types, providing potential therapeutic targets for cancer treatment.
References:
1. Zhou, Ting, Ke, Zhiqiang, Ma, Qianqian, Cheng, Xiyao, Su, Zhengding. 2023. Molecular mechanism of CCDC106 regulating the p53-Mdm2/MdmX signaling axis. In Scientific reports, 13, 21892. doi:10.1038/s41598-023-47808-z. https://pubmed.ncbi.nlm.nih.gov/38081879/
2. Zhao, Na, Wang, Chen, Guo, Peng, Bhawal, Ujjal K, Liu, Yang. . CCDC106 promotes the proliferation and invasion of ovarian cancer cells by suppressing p21 transcription through a p53-independent pathway. In Bioengineered, 13, 10956-10972. doi:10.1080/21655979.2022.2066759. https://pubmed.ncbi.nlm.nih.gov/35484984/
3. Zhang, Xiupeng, Zheng, Qin, Wang, Chen, Qiu, Xueshan, Wang, Enhua. . CCDC106 promotes non-small cell lung cancer cell proliferation. In Oncotarget, 8, 26662-26670. doi:10.18632/oncotarget.15792. https://pubmed.ncbi.nlm.nih.gov/28460455/
4. Cao, Canhui, Hong, Ping, Huang, Xingyu, Li, Guoliang, Wu, Peng. 2020. HPV-CCDC106 integration alters local chromosome architecture and hijacks an enhancer by three-dimensional genome structure remodeling in cervical cancer. In Journal of genetics and genomics = Yi chuan xue bao, 47, 437-450. doi:10.1016/j.jgg.2020.05.006. https://pubmed.ncbi.nlm.nih.gov/33023834/
5. Ning, Yichong, Wang, Chunqing, Liu, Xin, Zhou, Jianlin, Zhou, Chang. 2019. CK2-mediated CCDC106 phosphorylation is required for p53 degradation in cancer progression. In Journal of experimental & clinical cancer research : CR, 38, 131. doi:10.1186/s13046-019-1137-8. https://pubmed.ncbi.nlm.nih.gov/30885251/
6. Zhi, Wenhua, Wei, Ye, Lazare, Cordelle, Cao, Canhui, Wu, Peng. 2022. HPV-CCDC106 integration promotes cervical cancer progression by facilitating the high expression of CCDC106 after HPV E6 splicing. In Journal of medical virology, 95, e28009. doi:10.1002/jmv.28009. https://pubmed.ncbi.nlm.nih.gov/35854676/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen