C57BL/6NCya-Picalmem1flox/Cya
Common Name:
Picalm-flox
Product ID:
S-CKO-07651
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Picalm-flox
Strain ID
CKOCMP-233489-Picalm-B6N-VA
Gene Name
Product ID
S-CKO-07651
Gene Alias
CALM; CLTH; PAP180; fit-1; fit1; mKIAA4114
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Picalmem1flox/Cya mice (Catalog S-CKO-07651) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000207484
NCBI RefSeq
NM_146194
Target Region
Exon 5
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Picalm, short for Phosphatidylinositol binding clathrin-assembly protein, is a clathrin-adaptor protein. It plays a critical role in clathrin-mediated endocytosis and autophagy, being involved in pathways related to these processes. Picalm has been associated with various biological processes and diseases, making it an important gene for study. Genetic models, such as gene knockout (KO) or conditional knockout (CKO) mouse models, can help reveal its functions [1,2,4,5].
In a murine tauopathy model, reducing Picalm (by generating Tg30xPicalm+/- mice through crossing Tg30 tau transgenic mice with Picalm-haploinsufficient mice) exacerbated tau pathologies and tau-mediated neurodegeneration. These mice developed more severe motor deficits, had higher pathological tau levels, increased neurofibrillary tangles, and abnormal autophagy markers compared to Tg30 mice, suggesting Picalm expression changes as a risk factor for tau pathology development [5]. Also, genetic depletion of Picalm in a doxorubicin-induced cardiotoxicity mouse model reduced amyloid β-peptide generation, alleviating the cardiotoxicity. In this model, Picalm was upregulated only in cardiomyocytes with doxorubicin-induced cardiotoxicity, and its increase led to higher amyloid β-peptide production and greater cardiomyocyte membrane permeability [3].
In conclusion, Picalm is essential for processes like clathrin-mediated endocytosis and autophagy. Model-based research, especially KO mouse models, has revealed its role in diseases such as Alzheimer's disease-related tauopathies and anthracycline-induced cardiotoxicity. Understanding Picalm's functions through these models provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Ando, Kunie, Nagaraj, Siranjeevi, Küçükali, Fahri, Brion, Jean-Pierre, Leroy, Karelle. 2022. PICALM and Alzheimer's Disease: An Update and Perspectives. In Cells, 11, . doi:10.3390/cells11243994. https://pubmed.ncbi.nlm.nih.gov/36552756/
2. Xu, Wei, Tan, Lan, Yu, Jin-Tai. 2014. The Role of PICALM in Alzheimer's Disease. In Molecular neurobiology, 52, 399-413. doi:10.1007/s12035-014-8878-3. https://pubmed.ncbi.nlm.nih.gov/25186232/
3. Bao, Mengni, Hua, Xiumeng, Chen, Xiao, Yue, Guangxin, Song, Jiangping. 2024. PICALM Regulating the Generation of Amyloid β-Peptide to Promote Anthracycline-Induced Cardiotoxicity. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2401945. doi:10.1002/advs.202401945. https://pubmed.ncbi.nlm.nih.gov/38935046/
4. Hattersley, Kathryn J, Carosi, Julian M, Hein, Leanne K, Bensalem, Julien, Sargeant, Timothy J. 2021. PICALM regulates cathepsin D processing and lysosomal function. In Biochemical and biophysical research communications, 570, 103-109. doi:10.1016/j.bbrc.2021.07.024. https://pubmed.ncbi.nlm.nih.gov/34311200/
5. Ando, Kunie, De Decker, Robert, Vergara, Cristina, Leroy, Karelle, Brion, Jean-Pierre. 2020. Picalm reduction exacerbates tau pathology in a murine tauopathy model. In Acta neuropathologica, 139, 773-789. doi:10.1007/s00401-020-02125-x. https://pubmed.ncbi.nlm.nih.gov/31925534/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen