C57BL/6JCya-Tnrc6aem1flox/Cya
Common Name
Tnrc6a-flox
Product ID
S-CKO-07680
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-233833-Tnrc6a-B6J-VA
When using this mouse strain in a publication, please cite “Tnrc6a-flox Mouse (Catalog S-CKO-07680) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tnrc6a-flox
Strain ID
CKOCMP-233833-Tnrc6a-B6J-VA
Gene Name
Product ID
S-CKO-07680
Gene Alias
GW182, Tnrc6, CAGH26, 2010321I05Rik, 3110054G10Rik, D130023A07Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 7
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000094053
NCBI RefSeq
NM_144925
Target Region
Exon 7~8
Size of Effective Region
~2.0 kb
Overview of Gene Research
Tnrc6a, also known as GW182 paralog, is an essential protein for microRNA (miRNA)-mediated gene silencing. It functions as a platform protein in the cytoplasm, interacting with Argonaute protein for RNA silencing and with decapping enzymes or deadenylation protein complexes to induce mRNA degradation. It also shuttles between the cytoplasm and nucleus, potentially playing a role in navigating Ago protein into the nucleus for miRNA-mediated gene silencing [4,5].
In a rat model of myocardial infarction, abnormal expression of Tnrc6a was observed. As the disease progressed, Tnrc6a protein expressed abnormally, up-regulating miR-21 after the 3rd and 5th week of infarction, which in turn down-regulated PTEN, contributing to the development of myocardial infarction [1].
In non-small cell lung cancer (NSCLC), LINC01082 inhibits NSCLC cell proliferation, migration, and invasion by binding to miR-543, which targets Tnrc6a. TNRC6A was downregulated in NSCLC cells [2].
In hepatocellular carcinoma, miR-652-3p promotes metastasis by inhibiting TNRC6A expression, and high miR-652-3p and low TNRC6A expression are correlated with poor prognosis [3].
In mice, downregulation of TNRC6A in the prefrontal cortex (PFC) decreased the stability of miR-21-3p, up-regulating CRF and leading to anxiety-like behaviors [6].
In conclusion, Tnrc6a is crucial for miRNA-mediated gene silencing and is involved in various biological processes and disease conditions. Studies in different animal models have revealed its roles in myocardial infarction, NSCLC, hepatocellular carcinoma, and anxiety-like behaviors, highlighting its potential as a biomarker and therapeutic target for these diseases.
References：
1. Li, Liang, Chen, Qiang, Feng, Chao, Jin, Yongping, Xia, Shudong. 2019. Aberrant expression of TNRC6a and miR-21 during myocardial infarction. In 3 Biotech, 9, 285. doi:10.1007/s13205-019-1812-7. https://pubmed.ncbi.nlm.nih.gov/31245249/
2. Yang, Ran, Han, Jinli, Zhao, Song. 2023. LINC01082 Inhibits Non-Small Cell Lung Cancer by Targeting the miR-543/TNRC6A Axis. In Biochemical genetics, 61, 1585-1605. doi:10.1007/s10528-022-10313-5. https://pubmed.ncbi.nlm.nih.gov/36719626/
3. Chen, Wei, Ru, Junnan, Wu, Tong, Li, Yanhua, Yang, Beng. 2022. MiR-652-3p promotes malignancy and metastasis of cancer cells via inhibiting TNRC6A in hepatocellular carcinoma. In Biochemical and biophysical research communications, 640, 1-11. doi:10.1016/j.bbrc.2022.11.100. https://pubmed.ncbi.nlm.nih.gov/36495604/
4. Nishi, Kenji, Nishi, Ai, Nagasawa, Tatsuya, Ui-Tei, Kumiko. 2012. Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. In RNA (New York, N.Y.), 19, 17-35. doi:10.1261/rna.034769.112. https://pubmed.ncbi.nlm.nih.gov/23150874/
5. Suzawa, Masataka, Noguchi, Kentaro, Nishi, Kenji, Oyama, Masaaki, Ui-Tei, Kumiko. 2017. Comprehensive Identification of Nuclear and Cytoplasmic TNRC6A-Associating Proteins. In Journal of molecular biology, 429, 3319-3333. doi:10.1016/j.jmb.2017.04.017. https://pubmed.ncbi.nlm.nih.gov/28478284/
6. Lu, Gui-Feng, Yang, Xin, Xiao, Zhi, Huang, Meng-Qi, Geng, Fei. 2024. Prefrontal TNRC6A mediates anxiety-like behaviour by regulating CRF through the maintenance of miR-21-3p stability. In Neuropharmacology, 262, 110194. doi:10.1016/j.neuropharm.2024.110194. https://pubmed.ncbi.nlm.nih.gov/39424169/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.