C57BL/6JCya-Prr14em1flox/Cya
Common Name:
Prr14-flox
Product ID:
S-CKO-07692
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Prr14-flox
Strain ID
CKOCMP-233895-Prr14-B6J-VA
Gene Name
Product ID
S-CKO-07692
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prr14em1flox/Cya mice (Catalog S-CKO-07692) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033095
NCBI RefSeq
NM_145589
Target Region
Exon 1~11
Size of Effective Region
~5.3 kb
Detailed Document
Overview of Gene Research
Prr14, or Proline Rich 14, is a protein that plays a crucial role in organizing H3K9me3-modified heterochromatin at the nuclear lamina [1,4,7]. It dynamically associates with both the nuclear lamina and heterochromatin, and its two HP1-binding sites contribute to this association [1]. It also seems to be involved in multiple signaling pathways, such as the PI3K/Akt/mTOR pathway, which are important in various biological processes and disease-related events [2,5,6,8].
Specific knockout (KO) of skeletal muscle Prr14 in mice led to muscle atrophy. This demonstrated that Prr14 is crucial for mediating mechanotransduction, regulating MEF2C's activity, and maintaining myofiber identity [3]. In cancer research, RNA interfering (RNAi)-mediated inhibition of Prr14 in cutaneous squamous cell carcinoma (cSCC) cells suppressed cell proliferation, migration, and invasion while promoting apoptosis, suggesting its role as an oncogene in cSCC through the PI3K/Akt/mTOR signal pathway [2]. Similarly, in colon cancer, down-regulation of Prr14 inhibited cell growth, migration, invasion, and the epithelial-mesenchymal transition (EMT) process [5]. In breast cancer, Prr14 overexpression promoted cell proliferation and tumor formation by activating the PI3-kinase/Akt/mTOR pathway and inhibiting the CHEK2 pathway [6].
In conclusion, Prr14 is essential for the organization of heterochromatin at the nuclear lamina. Through gene-knockout models, its significance in skeletal muscle function and as an oncogene in multiple cancer types has been revealed. These findings offer new therapeutic avenues for skeletal muscle pathologies and cancer treatment.
References:
1. Kiseleva, Anna A, Cheng, Yu-Chia, Smith, Cheryl L, Katz, Richard A, Poleshko, Andrey. . PRR14 organizes H3K9me3-modified heterochromatin at the nuclear lamina. In Nucleus (Austin, Tex.), 14, 2165602. doi:10.1080/19491034.2023.2165602. https://pubmed.ncbi.nlm.nih.gov/36633363/
2. Zhang, Lili, Yang, Jie, Ji, Zhoujing, Zhang, Jie, Yang, Shengju. 2023. PRR14 acts a novel oncogene activating the PI3K signal pathway in human cutaneous squamous cell carcinoma. In Journal of Cancer, 14, 1531-1540. doi:10.7150/jca.83695. https://pubmed.ncbi.nlm.nih.gov/37325059/
3. Yang, Mei, Wang, Jiajie, Liu, Zhongyue, Li, Zhihong. 2024. PRR14 mediates mechanotransduction and regulates myofiber identity via MEF2C in skeletal muscle. In Metabolism: clinical and experimental, 164, 156109. doi:10.1016/j.metabol.2024.156109. https://pubmed.ncbi.nlm.nih.gov/39706290/
4. Dunlevy, Kelly L, Medvedeva, Valentina, Wilson, Jade E, Poleshko, Andrey, Katz, Richard A. 2020. The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting. In Journal of cell science, 133, . doi:10.1242/jcs.240416. https://pubmed.ncbi.nlm.nih.gov/32317397/
5. Li, Fangfang, Zhang, Chundong, Fu, Lijuan. 2019. PRR14 overexpression promotes cell growth, epithelial to mesenchymal transition and metastasis of colon cancer via the AKT pathway. In PloS one, 14, e0218839. doi:10.1371/journal.pone.0218839. https://pubmed.ncbi.nlm.nih.gov/31596887/
6. Ren, Xiaolei, Long, Meijun, Li, Zhihong, Qi, Lin, Yang, Mei. 2020. Oncogene PRR14 promotes breast cancer through activation of PI3K signal pathway and inhibition of CHEK2 pathway. In Cell death & disease, 11, 464. doi:10.1038/s41419-020-2640-8. https://pubmed.ncbi.nlm.nih.gov/32541902/
7. Kiseleva, Anna A, Poleshko, Andrey. 2023. The secret life of chromatin tethers. In FEBS letters, 597, 2782-2790. doi:10.1002/1873-3468.14685. https://pubmed.ncbi.nlm.nih.gov/37339933/
8. Yang, M, Lewinska, M, Fan, X, Zhu, J, Yuan, Z-M. 2016. PRR14 is a novel activator of the PI3K pathway promoting lung carcinogenesis. In Oncogene, 35, 5527-5538. doi:10.1038/onc.2016.93. https://pubmed.ncbi.nlm.nih.gov/27041574/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen