C57BL/6JCya-Setd1aem1flox/Cya
Common Name:
Setd1a-flox
Product ID:
S-CKO-07696
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Setd1a-flox
Strain ID
CKOCMP-233904-Setd1a-B6J-VA
Gene Name
Product ID
S-CKO-07696
Gene Alias
KMT2F; Nsccn1; mKIAA0339; mNSC1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Setd1aem1flox/Cya mice (Catalog S-CKO-07696) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000047157
NCBI RefSeq
NM_178029
Target Region
Exon 4
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Setd1a, a lysine-methyltransferase, is a key enzyme involved in histone H3 lysine 4 (H3K4) methylation. This methylation is crucial for chromatin-mediated regulation of gene expression, participating in various cellular processes such as cell cycle control, differentiation, and DNA damage repair [2,4]. It is also associated with multiple biological pathways and has overall significance in development and disease [3]. Genetic models, especially mouse models, have been valuable for studying its functions.
In Setd1a-deficient mice, which carry a heterozygous loss-of-function mutation, there are alterations in axonal branching and cortical synaptic dynamics, along with working memory deficits, recapitulating schizophrenia-related phenotypes [1]. Reinstating Setd1a expression in adulthood can rescue these cognitive deficits. Moreover, LSD1, identified as a major counteracting demethylase for Setd1a, and its pharmacological antagonism can fully rescue the behavioral and morphological deficits in these mice [1]. In Drosophila, the SETD1A orthologue is required in post-mitotic neurons of the fly brain for normal memory, suggesting its role in post-development neuronal function [2].
In conclusion, Setd1a is essential for normal neuronal function, as demonstrated through model-based research. The Setd1a-deficient mouse models have significantly contributed to understanding its role in schizophrenia-related phenotypes, providing insights into how Setd1a mutations may predispose to schizophrenia and pointing to potential therapeutic interventions [1].
References:
1. Mukai, Jun, Cannavò, Enrico, Crabtree, Gregg W, Xu, Bin, Gogos, Joseph A. 2019. Recapitulation and Reversal of Schizophrenia-Related Phenotypes in Setd1a-Deficient Mice. In Neuron, 104, 471-487.e12. doi:10.1016/j.neuron.2019.09.014. https://pubmed.ncbi.nlm.nih.gov/31606247/
2. Kummeling, Joost, Stremmelaar, Diante E, Raun, Nicholas, Kramer, Jamie M, Kleefstra, Tjitske. 2020. Characterization of SETD1A haploinsufficiency in humans and Drosophila defines a novel neurodevelopmental syndrome. In Molecular psychiatry, 26, 2013-2024. doi:10.1038/s41380-020-0725-5. https://pubmed.ncbi.nlm.nih.gov/32346159/
3. Kranz, Andrea, Anastassiadis, Konstantinos. 2020. The role of SETD1A and SETD1B in development and disease. In Biochimica et biophysica acta. Gene regulatory mechanisms, 1863, 194578. doi:10.1016/j.bbagrm.2020.194578. https://pubmed.ncbi.nlm.nih.gov/32389824/
4. Bayley, Rachel, Borel, Valerie, Moss, Rhiannon J, Boulton, Simon J, Higgs, Martin R. 2022. H3K4 methylation by SETD1A/BOD1L facilitates RIF1-dependent NHEJ. In Molecular cell, 82, 1924-1939.e10. doi:10.1016/j.molcel.2022.03.030. https://pubmed.ncbi.nlm.nih.gov/35439434/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen