C57BL/6JCya-Cnot1em1flox/Cya
Common Name:
Cnot1-flox
Product ID:
S-CKO-07747
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Cnot1-flox
Strain ID
CKOCMP-234594-Cnot1-B6J-VA
Gene Name
Product ID
S-CKO-07747
Gene Alias
6030411K04Rik; D830048B13
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cnot1em1flox/Cya mice (Catalog S-CKO-07747) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000211887
NCBI RefSeq
NM_001205226
Target Region
Exon 5~6
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
CNOT1, a member of the CCR4-NOT complex, is a master regulator in gene expression, RNA deadenylation, and protein ubiquitination [1]. This complex functions in the cytoplasm as the main mRNA deadenylase in constitutive and regulated mRNA decay pathways [3]. CNOT1, encoding the scaffold protein of the CCR4-NOT deadenylase complex, is involved in multiple biological processes, such as brain development, circadian rhythm regulation, and pancreatic development [1,2,4]. Genetic models like Drosophila and mouse models have been crucial for studying its functions.
In Drosophila, knockdown of CNOT1 led to learning and memory defects, and human wild-type CNOT1 could rescue this phenotype, suggesting that CNOT1 impairment causes neurodevelopmental delay [1]. In mice, CNOT1 deficiency resulted in circadian period lengthening and alterations in the mRNA and protein expression patterns of clock genes, mainly Per2, indicating its role in regulating the mammalian circadian clock [2].
In conclusion, CNOT1 is essential in various biological processes. Model-based research, especially the use of Drosophila and mouse models, has revealed its key role in neurodevelopmental delay and circadian behavior regulation. Understanding CNOT1 functions can provide insights into the underlying mechanisms of related diseases and potentially offer new therapeutic targets.
References:
1. Vissers, Lisenka E L M, Kalvakuri, Sreehari, de Boer, Elke, Bodmer, Rolf, de Brouwer, Arjan P M. 2020. De Novo Variants in CNOT1, a Central Component of the CCR4-NOT Complex Involved in Gene Expression and RNA and Protein Stability, Cause Neurodevelopmental Delay. In American journal of human genetics, 107, 164-172. doi:10.1016/j.ajhg.2020.05.017. https://pubmed.ncbi.nlm.nih.gov/32553196/
2. Mohamed, Haytham Mohamed Aly, Takahashi, Akinori, Nishijima, Saori, Okamura, Hitoshi, Yamamoto, Tadashi. 2021. CNOT1 regulates circadian behaviour through Per2 mRNA decay in a deadenylation-dependent manner. In RNA biology, 19, 703-718. doi:10.1080/15476286.2022.2071026. https://pubmed.ncbi.nlm.nih.gov/35510877/
3. Mauxion, Fabienne, Basquin, Jérôme, Ozgur, Sevim, Séraphin, Bertrand, Conti, Elena. 2022. The human CNOT1-CNOT10-CNOT11 complex forms a structural platform for protein-protein interactions. In Cell reports, 42, 111902. doi:10.1016/j.celrep.2022.111902. https://pubmed.ncbi.nlm.nih.gov/36586408/
4. de Queiroz Júnior, Amaro Freire, Sanseverino, Maria Teresa Vieira, Collares, Marcus Vinicius Martins, Artigalás, Osvaldo, Félix, Têmis Maria. 2024. CNOT1 p.Arg535Cys variant in holoprosencephaly with late onset diabetes mellitus. In American journal of medical genetics. Part A, 194, e63836. doi:10.1002/ajmg.a.63836. https://pubmed.ncbi.nlm.nih.gov/39149840/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen