C57BL/6JCya-Scapem1flox/Cya
Common Name:
Scap-flox
Product ID:
S-CKO-07859
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Scap-flox
Strain ID
CKOCMP-235623-Scap-B6J-VA
Gene Name
Product ID
S-CKO-07859
Gene Alias
9530044G19; mKIAA0199
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Scapem1flox/Cya mice (Catalog S-CKO-07859) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000098350
NCBI RefSeq
NM_001001144
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Scap, short for SREBP cleavage-activating protein, is a polytopic membrane protein. It serves as a crucial regulator in controlling the cholesterol content of membranes in mammalian cells. Scap binds to sterol regulatory element-binding proteins (SREBPs) and transports them from the endoplasmic reticulum (ER) to the Golgi for proteolytic processing. This process is part of the cholesterol homeostasis pathway, which is vital for maintaining normal cellular lipid levels [1].
In murine models, deleting Scap in the liver (a form of KO or CKO) exacerbated liver injury, fibrosis, and carcinogenesis in non-alcoholic steatohepatitis (NASH), despite reducing hepatic steatosis. This was due to alterations in fatty acid composition of phosphatidylcholines, leading to ER stress and hepatocyte injury [2]. Also, macrophage-specific knockout of Scap in mice attenuated Paigen diet-induced meta-inflammation and ectopic lipid deposition by reducing the activation of the STING-NF-κB pathway, suggesting Scap's role in lean NAFLD pathogenesis [3].
In conclusion, Scap is essential for regulating cholesterol homeostasis and lipid metabolism. The study of Scap using KO/CKO mouse models has provided insights into its role in diseases such as NASH and lean NAFLD, highlighting its potential as a therapeutic target for these metabolic disorders.
References:
1. Brown, Michael S, Radhakrishnan, Arun, Goldstein, Joseph L. 2017. Retrospective on Cholesterol Homeostasis: The Central Role of Scap. In Annual review of biochemistry, 87, 783-807. doi:10.1146/annurev-biochem-062917-011852. https://pubmed.ncbi.nlm.nih.gov/28841344/
2. Kawamura, Satoshi, Matsushita, Yuki, Kurosaki, Shigeyuki, Koike, Kazuhiko, Nakagawa, Hayato. . Inhibiting SCAP/SREBP exacerbates liver injury and carcinogenesis in murine nonalcoholic steatohepatitis. In The Journal of clinical investigation, 132, . doi:10.1172/JCI151895. https://pubmed.ncbi.nlm.nih.gov/35380992/
3. Huang, Xinyu, Yao, Yingcheng, Hou, Xiaoli, Li, Danyang, Chen, Yaxi. 2022. Macrophage SCAP Contributes to Metaflammation and Lean NAFLD by Activating STING-NF-κB Signaling Pathway. In Cellular and molecular gastroenterology and hepatology, 14, 1-26. doi:10.1016/j.jcmgh.2022.03.006. https://pubmed.ncbi.nlm.nih.gov/35367665/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen