C57BL/6JCya-Usp11em1flox/Cya
Common Name:
Usp11-flox
Product ID:
S-CKO-07884
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Usp11-flox
Strain ID
CKOCMP-236733-Usp11-B6J-VA
Gene Name
Product ID
S-CKO-07884
Gene Alias
6230415D12Rik; mKIAA4085
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Usp11em1flox/Cya mice (Catalog S-CKO-07884) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033383
NCBI RefSeq
NM_145628
Target Region
Exon 2~9
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Usp11, a member of the ubiquitin-specific protease family, is a deubiquitinating enzyme. It is involved in regulating multiple biological processes such as the cell cycle, DNA repair, and various signaling pathways. By deubiquitinating target proteins, Usp11 can affect their stability, function, and subsequent cellular responses, which is of great biological importance [6].
In spinal cord ischemia-reperfusion injury, knockout of Usp11 (Usp11-/Y) significantly decreased neuronal cell ferroptosis and promoted functional recovery in mice, suggesting Usp11 promotes autophagy-dependent ferroptosis in this context [1]. In intervertebral disc degeneration, knockout of Usp11 in vivo (Usp11-/-) resulted in exacerbated disease and poor pain-related behavioral scores, while its overexpression ameliorated oxidative stress-induced ferroptosis by stabilizing Sirt3 [2]. In hepatocellular carcinoma, knockdown of Usp11 inhibited its metastasis-promoting effect via the eEF1A1/SP1/HGF-dependent EMT pathway [3]. In radiation-induced pneumonitis, Usp11-/-mice showed alleviated disease progression with reduced pro-inflammatory cytokine expression, decreased pulmonary vessel permeability, and less immune cell infiltration [4]. In colorectal cancer, depletion of Usp11 increased cell sensitivity to ferroptosis, as it stabilizes LSH through deubiquitination to inhibit ferroptosis [5]. In prostate cancer, Usp11 knockdown significantly inhibited cell growth, and it promotes cancer progression by up-regulating AR and c-Myc activities [7].
In conclusion, Usp11 plays diverse and crucial roles in multiple biological processes and disease conditions. Gene knockout mouse models have been instrumental in revealing its functions, such as in ferroptosis-related diseases like spinal cord ischemia-reperfusion injury, intervertebral disc degeneration, and colorectal cancer, as well as in cancer metastasis and progression in hepatocellular carcinoma and prostate cancer. Understanding Usp11's functions through these models provides potential therapeutic targets for these diseases.
References：
1. Rong, Yuluo, Fan, Jin, Ji, Chengyue, Cai, Weihua, Liu, Wei. 2021. USP11 regulates autophagy-dependent ferroptosis after spinal cord ischemia-reperfusion injury by deubiquitinating Beclin 1. In Cell death and differentiation, 29, 1164-1175. doi:10.1038/s41418-021-00907-8. https://pubmed.ncbi.nlm.nih.gov/34839355/
2. Zhu, Jian, Sun, Ruping, Sun, Kaiqiang, Kong, Fanqi, Shi, Jiangang. 2023. The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3. In Redox biology, 62, 102707. doi:10.1016/j.redox.2023.102707. https://pubmed.ncbi.nlm.nih.gov/37099926/
3. Chen, Jin, Ning, Deng, Du, Pengcheng, Zhang, Bixiang, Chen, Xiaoping. 2023. USP11 potentiates HGF/AKT signaling and drives metastasis in hepatocellular carcinoma. In Oncogene, 43, 123-135. doi:10.1038/s41388-023-02847-8. https://pubmed.ncbi.nlm.nih.gov/37973952/
4. Tang, Yiting, Wang, Tingya, Gu, Liming, Cao, Jianping, Jiao, Yang. 2024. USP11 Exacerbates Radiation-Induced Pneumonitis by Activating Endothelial Cell Inflammatory Response via OTUD5-STING Signaling. In International journal of radiation oncology, biology, physics, 119, 1261-1274. doi:10.1016/j.ijrobp.2024.01.220. https://pubmed.ncbi.nlm.nih.gov/38364946/
5. Duan, Junyi, Huang, Daoyuan, Liu, Cheng, Wang, Weiping, Shao, Genze. 2023. USP11-mediated LSH deubiquitination inhibits ferroptosis in colorectal cancer through epigenetic activation of CYP24A1. In Cell death & disease, 14, 402. doi:10.1038/s41419-023-05915-9. https://pubmed.ncbi.nlm.nih.gov/37414755/
6. Guo, Tuanjie, Tang, Heting, Yuan, Zhihao, Zhang, Encheng, Wang, Xiang. 2022. The Dual Role of USP11 in Cancer. In Journal of oncology, 2022, 9963905. doi:10.1155/2022/9963905. https://pubmed.ncbi.nlm.nih.gov/35359344/
7. Pornour, Majid, Jeon, Hee-Young, Ryu, Hyunju, Barbieri, Christopher, Qi, Jianfei. 2024. USP11 promotes prostate cancer progression by up-regulating AR and c-Myc activity. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2403331121. doi:10.1073/pnas.2403331121. https://pubmed.ncbi.nlm.nih.gov/39052835/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen