C57BL/6JCya-Tmem185aem1flox/Cya
Common Name:
Tmem185a-flox
Product ID:
S-CKO-07892
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tmem185a-flox
Strain ID
CKOCMP-236848-Tmem185a-B6J-VA
Gene Name
Product ID
S-CKO-07892
Gene Alias
ee3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmem185aem1flox/Cya mice (Catalog S-CKO-07892) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000114630
NCBI RefSeq
NM_001357750
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Tmem185a, a gene with its specific functions yet to be fully elaborated in a comprehensive manner. Based on current knowledge, it may be involved in various biological processes as its copy number can be affected by genomic rearrangements, like in a case of a complex rearrangement causing severe hemophilia A where TMEM185A was duplicated along with other genes, though its biological effect in this context did not seem dosage-dependent [3].
In cancer research, TMEM185A was identified as one of the neoantigens specifically present in lymph-node-positive breast cancers, making it a potential target for vaccine design [1]. In urinary bladder cancer, it was part of a four-gene signature (along with TMPRSS11E, SCEL, KRT78) that could predict overall survival, and this four-gene risk score had potential clinical implications in selecting high-risk patients for aggressive therapy [2]. In stage III serous ovarian carcinoma, TMEM185A was among the down-regulated genes identified by an annealing control primer system, and these differentially expressed genes might help predict patient prognosis and suggest new treatment targets [4].
In conclusion, TMEM185A shows potential significance in cancer-related biological processes, especially in breast, urinary bladder, and ovarian cancers. Its identification as a neoantigen and part of gene signatures in different cancers highlights its potential as a therapeutic target and prognostic marker, although more in-depth functional studies, potentially using gene knockout (KO) or conditional knockout (CKO) mouse models, are needed to fully understand its role in these disease conditions.
References:
1. Wang, Zhigang, Liu, Wei, Chen, Chong, Luo, Yunping, Zhang, Bailin. 2019. Low mutation and neoantigen burden and fewer effector tumor infiltrating lymphocytes correlate with breast cancer metastasization to lymph nodes. In Scientific reports, 9, 253. doi:10.1038/s41598-018-36319-x. https://pubmed.ncbi.nlm.nih.gov/30670769/
2. Chen, Siteng, Zhang, Ning, Shao, Jialiang, Wang, Tao, Wang, Xiang. 2019. A novel gene signature combination improves the prediction of overall survival in urinary bladder cancer. In Journal of Cancer, 10, 5744-5753. doi:10.7150/jca.30307. https://pubmed.ncbi.nlm.nih.gov/31737111/
3. Sheen, Campbell R, Jewell, Ursula R, Morris, Christine M, Smith, Mark P, Chen, Jian-Min. . Double complex mutations involving F8 and FUNDC2 caused by distinct break-induced replication. In Human mutation, 28, 1198-206. doi:. https://pubmed.ncbi.nlm.nih.gov/17683067/
4. Kim, Yun-Sook, Hwan, Jin Do, Bae, Sumi, Bae, Dong-Han, Shick, Woong Ahn. 2010. Identification of differentially expressed genes using an annealing control primer system in stage III serous ovarian carcinoma. In BMC cancer, 10, 576. doi:10.1186/1471-2407-10-576. https://pubmed.ncbi.nlm.nih.gov/20969748/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen