C57BL/6JCya-Agr2em1flox/Cya
Common Name:
Agr2-flox
Product ID:
S-CKO-07976
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Agr2-flox
Strain ID
CKOCMP-23795-Agr2-B6J-VA
Gene Name
Product ID
S-CKO-07976
Gene Alias
Agr2h; Gob-4; HAG-2; XAG-2; mAG-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Agr2em1flox/Cya mice (Catalog S-CKO-07976) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020898
NCBI RefSeq
NM_011783
Target Region
Exon 2~4
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
AGR2, or anterior gradient-2, is a 20 kDa protein crucial for oxidative protein folding in the endoplasmic reticulum [4]. It's involved in several signal transduction pathways essential for cell survival and was initially discovered in Xenopus laevis, where it contributes to embryonic development. In mammals, it has diverse functions and is associated with various biological processes and diseases [4].
In colorectal cancer (CRC), neutrophil-specific Agr2 knockout (Agr2f/f;Mrp-Cre) mice were used to study the role of tumour-associated neutrophils (TANs)-secreted AGR2. Results showed that TANs are a major source of secreted AGR2 in the CRC tumour microenvironment (TME), and TAN-secreted AGR2 promotes CRC cell migration. Neutrophils-specific ablation of Agr2 in mice ameliorated CRC liver metastases, unveiling a TAN-CRC cell crosstalk via the AGR2-CD98hc-xCT axis promoting metastasis [1]. In breast cancer, AGR2 is expressed in precancerous, primary, and metastatic tumours. lncRNA LINC02273 was found to epigenetically increase AGR2 transcription, promoting breast cancer metastasis in vitro and in vivo, and the combination of AGR2 and LINC02273 is an independent prognostic factor [2,3].
In summary, AGR2 is important for oxidative protein folding and cell survival-related signal transduction. Model-based research, especially using Agr2 knockout mouse models in CRC, has revealed its role in promoting cancer metastasis. In breast cancer, its association with lncRNA-mediated regulation and prognosis also highlights its significance in understanding cancer development and potential therapeutic targeting.
References：
1. Tian, Shaobo, Chu, Yanan, Hu, Jia, Wang, Lin, Wang, Zheng. 2022. Tumour-associated neutrophils secrete AGR2 to promote colorectal cancer metastasis via its receptor CD98hc-xCT. In Gut, 71, 2489-2501. doi:10.1136/gutjnl-2021-325137. https://pubmed.ncbi.nlm.nih.gov/35086885/
2. Zhang, Ke, Li, Yuan, Kong, Xiangyi, Chang, Hu, Xuan, Lixue. 2023. AGR2: a secreted protein worthy of attention in diagnosis and treatment of breast cancer. In Frontiers in oncology, 13, 1195885. doi:10.3389/fonc.2023.1195885. https://pubmed.ncbi.nlm.nih.gov/37197416/
3. Xiu, Bingqiu, Chi, Yayun, Liu, Lei, Huang, Shenglin, Wu, Jiong. 2019. LINC02273 drives breast cancer metastasis by epigenetically increasing AGR2 transcription. In Molecular cancer, 18, 187. doi:10.1186/s12943-019-1115-y. https://pubmed.ncbi.nlm.nih.gov/31856843/
4. Jach, Daria, Cheng, Yuzhu, Prica, Filip, Dumartin, Laurent, Crnogorac-Jurcevic, Tatjana. 2021. From development to cancer - an ever-increasing role of AGR2. In American journal of cancer research, 11, 5249-5262. doi:. https://pubmed.ncbi.nlm.nih.gov/34873459/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen