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C57BL/6JCya-Vash1em1flox/Cya
Common Name:
Vash1-flox
Product ID:
S-CKO-08017
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Vash1-flox
Strain ID
CKOCMP-238328-Vash1-B6J-VA
Gene Name
Vash1
Product ID
S-CKO-08017
Gene Alias
D930046M13Rik; G630009D10Rik; TTCP 1
Background
C57BL/6JCya
NCBI ID
238328
Modification
Conditional knockout
Chromosome
12
Phenotype
MGI:2442543
Document
Click here to download >>
Application
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More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Vash1em1flox/Cya mice (Catalog S-CKO-08017) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021681
NCBI RefSeq
NM_177354
Target Region
Exon 4~5
Size of Effective Region
~1.6 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Vash1, short for vasohibin-1, is a gene with multiple important functions. It is involved in the detyrosination/tyrosination cycle of α-tubulin, which is crucial for proper cell functioning. Vash1 forms a complex with small-vasohibin binding protein (SVBP) to mediate α-tubulin detyrosination, a post-translational modification of microtubules (MTs) that impacts MT-associated proteins and molecular motors [1,2,4,9,10]. Additionally, Vash1 is related to angiogenesis pathways, with its expression being influenced by vascular endothelial growth factor A (VEGFA) [5]. It also plays roles in various disease-related processes, including cancer and inflammation [3,6,7,8].

In ovarian cancer, Vash1 acts as a tumor-suppressing factor, with lower expression in neoplastic tissues compared to normal tissues. It affects overall survival (OS) and recurrence-free survival (RFS), negatively regulates tumor purity, and controls the tumor microenvironment through immunocyte infiltration. Functional enrichment analysis suggests it suppresses tumors by regulating the extracellular matrix receptor pathway, and in vivo experiments confirm its inhibition of ovarian cancer cell malignant phenotypes [3].

In lower-grade glioma (LGG), high Vash1 expression is associated with poor prognosis, WHO grade, IDH1 wild-type, and progressive disease. Knockdown of Vash1 in glioma cell lines increases cell proliferation, invasion, and migration, potentially by affecting microtubule formation and immune microenvironment remodeling [6].

In endometriosis, miR-143-3p targets Vash1 to activate the TGF-β signaling pathway, facilitating the motility and invasiveness of endometriotic stromal cells [7]. Porcine β-defensin 2 (PBD-2) interacts with intracellular Vash1 to inhibit the LPS-induced Akt/NF-κB signaling pathway and suppress inflammatory responses [8].

In conclusion, Vash1 is essential for microtubule detyrosination and has significant implications in multiple disease areas. Its role as a tumor suppressor in ovarian cancer and its association with prognosis in lower-grade glioma, along with its involvement in endometriosis-related cell motility and inflammation regulation, highlight its importance in understanding disease mechanisms. Studies on Vash1 contribute to a better understanding of these biological processes and may offer potential therapeutic targets.

References:
1. Ramirez-Rios, Sacnicte, Choi, Sung Ryul, Sanyal, Chadni, Steinmetz, Michel O, Moutin, Marie-Jo. 2022. VASH1-SVBP and VASH2-SVBP generate different detyrosination profiles on microtubules. In The Journal of cell biology, 222, . doi:10.1083/jcb.202205096. https://pubmed.ncbi.nlm.nih.gov/36512346/
2. Yue, Yang, Hotta, Takashi, Higaki, Takumi, Verhey, Kristen J, Ohi, Ryoma. 2023. Microtubule detyrosination by VASH1/SVBP is regulated by the conformational state of tubulin in the lattice. In Current biology : CB, 33, 4111-4123.e7. doi:10.1016/j.cub.2023.07.062. https://pubmed.ncbi.nlm.nih.gov/37716348/
3. Li, Yan, Meng, Liang, Lou, Ge. 2023. Revealing the inhibitory effect of VASH1 on ovarian cancer from multiple perspectives. In Cancer biology & therapy, 24, 2285817. doi:10.1080/15384047.2023.2285817. https://pubmed.ncbi.nlm.nih.gov/38010374/
4. Li, Faxiang, Li, Yang, Ye, Xuecheng, Rice, Luke M, Yu, Hongtao. 2020. Cryo-EM structure of VASH1-SVBP bound to microtubules. In eLife, 9, . doi:10.7554/eLife.58157. https://pubmed.ncbi.nlm.nih.gov/32773040/
5. Qiao, Sijing, Hou, Yue, Rong, Qing, Han, Bing, Liu, Peishu. 2023. Tregs are involved in VEGFA/ VASH1-related angiogenesis pathway in ovarian cancer. In Translational oncology, 32, 101665. doi:10.1016/j.tranon.2023.101665. https://pubmed.ncbi.nlm.nih.gov/37018867/
6. Aili, Yirizhati, Maimaitiming, Nuersimanguli, Maimaiti, Aierpati, Wang, Yongxin, Wang, Zengliang. 2022. Identification of VASH1 as a Potential Prognostic Biomarker of Lower-Grade Glioma by Quantitative Proteomics and Experimental Verification. In Journal of oncology, 2022, 2621969. doi:10.1155/2022/2621969. https://pubmed.ncbi.nlm.nih.gov/36504559/
7. Li, Na, Yi, Keyun, Li, Xia, Hu, Jiaxuan, Wang, Zhenhua. 2022. MiR-143-3p facilitates motility and invasiveness of endometriotic stromal cells by targeting VASH1/TGF-β signaling. In Reproductive biology, 22, 100592. doi:10.1016/j.repbio.2021.100592. https://pubmed.ncbi.nlm.nih.gov/34995817/
8. Huang, Chao, Sun, Yufan, Qiu, Xiuxiu, Zhou, Rui, Li, Lu. 2022. The Intracellular Interaction of Porcine β-Defensin 2 with VASH1 Alleviates Inflammation via Akt Signaling Pathway. In Journal of immunology (Baltimore, Md. : 1950), 208, 2795-2805. doi:10.4049/jimmunol.2100810. https://pubmed.ncbi.nlm.nih.gov/35688466/
9. Li, Lin, Williams, Preston, Gao, Zi, Wang, Yinsheng. . VEZF1-guanine quadruplex DNA interaction regulates alternative polyadenylation and detyrosinase activity of VASH1. In Nucleic acids research, 48, 11994-12003. doi:10.1093/nar/gkaa1092. https://pubmed.ncbi.nlm.nih.gov/33231681/
10. Adamopoulos, Athanassios, Landskron, Lisa, Heidebrecht, Tatjana, Brummelkamp, Thijn R, Perrakis, Anastassis. 2019. Crystal structure of the tubulin tyrosine carboxypeptidase complex VASH1-SVBP. In Nature structural & molecular biology, 26, 567-570. doi:10.1038/s41594-019-0254-6. https://pubmed.ncbi.nlm.nih.gov/31270470/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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