C57BL/6JCya-Ets2em1flox/Cya
Common Name:
Ets2-flox
Product ID:
S-CKO-08051
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ets2-flox
Strain ID
CKOCMP-23872-Ets2-B6J-VA
Gene Name
Product ID
S-CKO-08051
Gene Alias
Ets-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ets2em1flox/Cya mice (Catalog S-CKO-08051) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023612
NCBI RefSeq
NM_011809
Target Region
Exon 3~5
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Ets2, also known as V-ets erythroblastosis virus E26 oncogene homolog 2, is a transcription factor belonging to the E26 transformation-specific sequence (ETS) domain family. It plays crucial roles in regulating cell proliferation, differentiation, apoptosis, and tumorigenesis, and is involved in multiple signaling pathways such as the MAPK/Erk and calcineurin/NFAT pathways [1,2].
In cardiac hypertrophy, conditional deletion of Ets2 in mouse cardiomyocytes protects against pressure-overload induced cardiac hypertrophy, and silencing Ets2 in the hearts of calcineurin transgenic mice attenuates hypertrophic growth and contractile dysfunction. Ets2 forms a complex with NFAT to stimulate transcriptional activity of hypertrophy-stimulated genes, revealing its role in the pathogenesis of cardiac hypertrophy [1].
In renal fibrosis, knockdown of Ets2 abrogates TGF-β1-mediated expression of profibrogenic factors in human proximal tubular epithelial cells, suggesting that Ets2 promotes epithelial-to-mesenchymal transition in renal fibrosis by targeting JUNB transcription [3].
In conclusion, Ets2 is a key transcription factor involved in multiple biological processes and disease conditions. Gene knockout and conditional knockout mouse models have significantly contributed to understanding its role in cardiac hypertrophy and renal fibrosis. These functional studies provide important insights into the underlying molecular mechanisms of related diseases, potentially paving the way for new therapeutic strategies.
References:
1. Luo, Yuxuan, Jiang, Nan, May, Herman I, Gillette, Thomas G, Hill, Joseph A. 2021. Cooperative Binding of ETS2 and NFAT Links Erk1/2 and Calcineurin Signaling in the Pathogenesis of Cardiac Hypertrophy. In Circulation, 144, 34-51. doi:10.1161/CIRCULATIONAHA.120.052384. https://pubmed.ncbi.nlm.nih.gov/33821668/
2. Choi, Yunsik, Lee, Yuri, Kim, Jin Seo, Zhang, Peijing, Kim, Jongchan. 2023. USP39-Mediated Non-Proteolytic Control of ETS2 Suppresses Nuclear Localization and Activity. In Biomolecules, 13, . doi:10.3390/biom13101475. https://pubmed.ncbi.nlm.nih.gov/37892157/
3. Yao, Fang, Wang, Xiaojing, Cui, Zhong-Kai, Wu, Bingyi, Bai, Xiaochun. 2019. ETS2 promotes epithelial-to-mesenchymal transition in renal fibrosis by targeting JUNB transcription. In Laboratory investigation; a journal of technical methods and pathology, 100, 438-453. doi:10.1038/s41374-019-0331-9. https://pubmed.ncbi.nlm.nih.gov/31641227/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen