C57BL/6JCya-Tnpo1em1flox/Cya
Common Name:
Tnpo1-flox
Product ID:
S-CKO-08059
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tnpo1-flox
Strain ID
CKOCMP-238799-Tnpo1-B6J-VA
Gene Name
Product ID
S-CKO-08059
Gene Alias
D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tnpo1em1flox/Cya mice (Catalog S-CKO-08059) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000109401
NCBI RefSeq
NM_178716
Target Region
Exon 3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Tnpo1, also known as Transportin-1 and Karyopherin-β2, is a crucial member of the karyopherin-β family. It is mainly involved in nuclear import processes, facilitating the transport of specific proteins into the nucleus. This function is essential for various cellular processes and signaling pathways, such as those related to cell growth, differentiation, and immune response [1,3,4,5].
In cancer research, TNPO1 has been found to play oncogenic roles. In ARID1A-deficient gynecologic cancer, TNPO1-mediated nuclear import of ARID1B promotes tumor growth. Knocking down TNPO1 or ARID1B changes chromatin accessibility, leading to loss of certain histone markers, diminishing activated transcription factors of the AP-1 family, and inactivating the PI3K/AKT signaling pathway [1]. In esophageal cancer, circBCAR3 can upregulate TNPO1 by interacting with miR-27a-3p, accelerating tumorigenesis and metastasis [2]. In cervical cancer, TNPO1-mediated nuclear import of FUBP1 contributes to tumor immune evasion by increasing NRP1 expression [3].
In conclusion, TNPO1 is vital for protein nuclear import, significantly influencing cellular functions. Its role in cancer, as revealed by functional studies in relevant models, shows its potential as a therapeutic target in treating ARID1A-deficient gynecologic cancer, esophageal cancer, and cervical cancer. Understanding TNPO1 provides insights into cancer mechanisms and potential treatment strategies.
References：
1. Yang, Bikang, Chen, Jing, Li, Xiao, Zhang, Zhigang, Teng, Yincheng. 2021. TNPO1-mediated nuclear import of ARID1B promotes tumor growth in ARID1A-deficient gynecologic cancer. In Cancer letters, 515, 14-27. doi:10.1016/j.canlet.2021.05.016. https://pubmed.ncbi.nlm.nih.gov/34044070/
2. Xi, Yong, Shen, Yaxing, Wu, Donglei, Yu, Bentong, Shen, Weiyu. 2022. CircBCAR3 accelerates esophageal cancer tumorigenesis and metastasis via sponging miR-27a-3p. In Molecular cancer, 21, 145. doi:10.1186/s12943-022-01615-8. https://pubmed.ncbi.nlm.nih.gov/35840974/
3. Yang, BiKang, Chen, Jing, Teng, YinCheng. 2021. TNPO1-Mediated Nuclear Import of FUBP1 Contributes to Tumor Immune Evasion by Increasing NRP1 Expression in Cervical Cancer. In Journal of immunology research, 2021, 9994004. doi:10.1155/2021/9994004. https://pubmed.ncbi.nlm.nih.gov/33987449/
4. Guo, Lin, Kim, Hong Joo, Wang, Hejia, Taylor, J Paul, Shorter, James. . Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains. In Cell, 173, 677-692.e20. doi:10.1016/j.cell.2018.03.002. https://pubmed.ncbi.nlm.nih.gov/29677512/
5. Hofweber, Mario, Hutten, Saskia, Bourgeois, Benjamin, Madl, Tobias, Dormann, Dorothee. . Phase Separation of FUS Is Suppressed by Its Nuclear Import Receptor and Arginine Methylation. In Cell, 173, 706-719.e13. doi:10.1016/j.cell.2018.03.004. https://pubmed.ncbi.nlm.nih.gov/29677514/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen