C57BL/6JCya-G3bp2em1flox/Cya
Common Name:
G3bp2-flox
Product ID:
S-CKO-08062
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
G3bp2-flox
Strain ID
CKOCMP-23881-G3bp2-B6J-VA
Gene Name
Product ID
S-CKO-08062
Gene Alias
E430034L04Rik; G3BP; mKIAA0660
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-G3bp2em1flox/Cya mice (Catalog S-CKO-08062) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000169094
NCBI RefSeq
NM_001080794
Target Region
Exon 4~5
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
G3bp2, a member of the Ras-GAP SH3 domain binding proteins family, is an RNA-binding protein. It can bind with RNA or protein, regulating the nucleoplasmic shuttle. This protein is involved in multiple biological functions such as cell growth, differentiation, migration, and RNA and protein metabolism [1].
In tauopathies, G3bp2 directly interacts with Tau and inhibits Tau aggregation. Loss of G3bp2 in human neurons and brain organoids significantly elevates Tau pathology [2]. In non-small cell lung cancer, MG53, a TRIM protein family member, modulates G3bp2 activity. Knockdown of G3bp2 reduces the tumorigenicity of NSCLC cells [3]. In endothelial cells, G3bp2 is a critical oscillatory shear stress (OSS) regulated gene. G3bp2-/-Apoe-/-mice have decreased atherosclerotic lesions due to G3bp2 deficiency's impact on endothelial barrier function, monocyte adhesion, and pro-inflammatory cytokine levels [4]. In head and neck squamous carcinoma (HNSC), PRMT5-USP7-G3bp2 regulatory axis drives de novo lipogenesis and tumorigenesis [5]. In esophageal squamous cell carcinoma (ESCC), G3bp2, regulated by lncRNA LINC01554, facilitates metastasis by stabilizing HDGF transcript [6]. In pancreatic ductal adenocarcinoma (PDAC), knockdown of G3bp2 inhibits cell proliferation and invasion, and G3bp2 promotes gemcitabine resistance via regulating PDIA3-DKC1-hENT in a stress-granules-dependent manner [7]. In breast cancer, CAF-derived exosomal miR-92a downregulates G3bp2, enhancing cell migration and invasion [8].
In summary, G3bp2 is involved in a variety of biological processes and disease conditions. Studies using gene knockout or conditional knockout mouse models have revealed its role in neurodegenerative diseases like tauopathies, and in multiple cancers including lung, esophageal, pancreatic, and breast cancer, as well as in endothelial-related diseases such as atherosclerosis. These findings provide insights into potential therapeutic strategies targeting G3bp2 for these diseases.
References:
1. Jin, Ge, Zhang, Zhen, Wan, Jingjing, Liu, Xia, Zhang, Weidong. 2022. G3BP2: Structure and function. In Pharmacological research, 186, 106548. doi:10.1016/j.phrs.2022.106548. https://pubmed.ncbi.nlm.nih.gov/36336216/
2. Wang, Congwei, Terrigno, Marco, Li, Juan, Grüninger, Fiona, Jagasia, Ravi. 2023. Increased G3BP2-Tau interaction in tauopathies is a natural defense against Tau aggregation. In Neuron, 111, 2660-2674.e9. doi:10.1016/j.neuron.2023.05.033. https://pubmed.ncbi.nlm.nih.gov/37385246/
3. Li, Haichang, Lin, Pei-Hui, Gupta, Pranav, Merritt, Robert E, Ma, Jianjie. 2021. MG53 suppresses tumor progression and stress granule formation by modulating G3BP2 activity in non-small cell lung cancer. In Molecular cancer, 20, 118. doi:10.1186/s12943-021-01418-3. https://pubmed.ncbi.nlm.nih.gov/34521423/
4. Li, Tianhan, Qiu, Juhui, Jia, Tingting, Chen, Yaokai, Wang, Guixue. 2021. G3BP2 regulates oscillatory shear stress-induced endothelial dysfunction. In Genes & diseases, 9, 1701-1715. doi:10.1016/j.gendis.2021.11.003. https://pubmed.ncbi.nlm.nih.gov/36157502/
5. Wang, Nan, Li, Tianzi, Liu, Wanyu, Xu, Meilan, Liu, Xuekui. 2023. USP7- and PRMT5-dependent G3BP2 stabilization drives de novo lipogenesis and tumorigenesis of HNSC. In Cell death & disease, 14, 182. doi:10.1038/s41419-023-05706-2. https://pubmed.ncbi.nlm.nih.gov/36878903/
6. Zheng, Yinli, Wu, Jinjun, Deng, Ru, Li, Yan, Yun, Jingping. . G3BP2 regulated by the lncRNA LINC01554 facilitates esophageal squamous cell carcinoma metastasis through stabilizing HDGF transcript. In Oncogene, 41, 515-526. doi:10.1038/s41388-021-02073-0. https://pubmed.ncbi.nlm.nih.gov/34782720/
7. Xing, Fa-Liang, Li, Bo-Rui, Fang, Ying-Jin, Qin, Yi, Zhang, Bo. 2024. G3BP2 promotes tumor progression and gemcitabine resistance in PDAC via regulating PDIA3-DKC1-hENT in a stress granules-dependent manner. In Acta pharmacologica Sinica, 46, 474-488. doi:10.1038/s41401-024-01387-5. https://pubmed.ncbi.nlm.nih.gov/39289547/
8. Sheng, Zhimei, Wang, Xuejie, Ding, Xiaodi, Su, Wenxia, Zhang, Baogang. 2024. Exosomal miRNA-92a derived from cancer-associated fibroblasts promote invasion and metastasis in breast cancer by regulating G3BP2. In Cellular signalling, 119, 111182. doi:10.1016/j.cellsig.2024.111182. https://pubmed.ncbi.nlm.nih.gov/38640983/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen