C57BL/6JCya-Impdh2em1flox/Cya
Common Name:
Impdh2-flox
Product ID:
S-CKO-08097
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Impdh2-flox
Strain ID
CKOCMP-23918-Impdh2-B6J-VA
Gene Name
Product ID
S-CKO-08097
Gene Alias
IMPD; IMPD 2; IMPDH-II
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Impdh2em1flox/Cya mice (Catalog S-CKO-08097) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000081111
NCBI RefSeq
NM_011830
Target Region
Exon 2~9
Size of Effective Region
~3.4 kb
Detailed Document
Overview of Gene Research
IMPDH2, or inosine 5'-monophosphate dehydrogenase type II, is a key enzyme in the de novo biosynthesis of guanine nucleotides, a dopamine synthetic pathway. It's involved in multiple biological processes such as cell cycle regulation, DNA damage response, and GTP synthesis [1,2,3].
In various disease models, IMPDH2 has shown significant impacts. In colorectal cancer, its overexpression promotes cell proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT) via activation of the PI3K/AKT/mTOR and PI3K/AKT/FOXO1 pathways, while knockdown has the opposite effect [1]. In osteoclastogenesis, Impdh2 deficiency in mouse myeloid lineage cells reduces osteoclast number and alleviates ovariectomy-induced osteoporosis by impairing mitochondrial oxidative phosphorylation [4]. In hepatoblastoma, IMPDH2 overexpression is associated with poor patient outcomes, and its knockdown inhibits cell proliferation and induces cell cycle arrest [5].
In conclusion, IMPDH2 plays essential roles in processes like cell cycle regulation, GTP synthesis, and cell-specific functions. Studies using gene-knockout or knockdown models in various disease areas such as cancer and osteoporosis have revealed its significance, suggesting it could be a potential biomarker and therapeutic target for these diseases [1,4,5].
References:
1. Duan, Shiyu, Huang, Wenqing, Liu, Xiaoting, Song, Wen, Zhou, Jun. 2018. IMPDH2 promotes colorectal cancer progression through activation of the PI3K/AKT/mTOR and PI3K/AKT/FOXO1 signaling pathways. In Journal of experimental & clinical cancer research : CR, 37, 304. doi:10.1186/s13046-018-0980-3. https://pubmed.ncbi.nlm.nih.gov/30518405/
2. Espinar, Lorena, Garcia-Cao, Marta, Schmidt, Alisa, Montero, Joan, Sdelci, Sara. 2024. Nuclear IMPDH2 controls the DNA damage response by modulating PARP1 activity. In Nature communications, 15, 9515. doi:10.1038/s41467-024-53877-z. https://pubmed.ncbi.nlm.nih.gov/39532854/
3. Flores-Mendez, Marco, Ohl, Laura, Roule, Thomas, Ortiz-González, Xilma R, Akizu, Naiara. 2024. IMPDH2 filaments protect from neurodegeneration in AMPD2 deficiency. In EMBO reports, 25, 3990-4012. doi:10.1038/s44319-024-00218-2. https://pubmed.ncbi.nlm.nih.gov/39075237/
4. Xu, Cheng, Wei, Zhixin, Lv, Longfei, Akimoto, Yoshie, Hu, Zhangfeng. 2024. Impdh2 deficiency suppresses osteoclastogenesis through mitochondrial oxidative phosphorylation and alleviates ovariectomy-induced osteoporosis. In Biochemical and biophysical research communications, 727, 150317. doi:10.1016/j.bbrc.2024.150317. https://pubmed.ncbi.nlm.nih.gov/38959733/
5. Li, Linman, Wu, Yichi, Huang, Hong-Ting, Feng, Hao, Xia, Qiang. 2024. IMPDH2 suppression impedes cell proliferation by instigating cell cycle arrest and stimulates apoptosis in pediatric hepatoblastoma. In Journal of cancer research and clinical oncology, 150, 377. doi:10.1007/s00432-024-05858-4. https://pubmed.ncbi.nlm.nih.gov/39085725/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen