C57BL/6JCya-Katna1em1flox/Cya
Common Name:
Katna1-flox
Product ID:
S-CKO-08104
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Katna1-flox
Strain ID
CKOCMP-23924-Katna1-B6J-VA
Gene Name
Product ID
S-CKO-08104
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Katna1em1flox/Cya mice (Catalog S-CKO-08104) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000165806
NCBI RefSeq
--
Target Region
Exon 3
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
KATNA1, also known as katanin p60 ATPase-containing subunit A1, is a microtubule-cleaving enzyme. It is crucial for cytoskeletal rearrangements, which are involved in neural protrusion development, cell division, motility, and morphogenesis [1,2,3,5]. Microtubule severing is an essential process for the reorganization of microtubules based on cellular needs, such as during mitosis in dividing cells and in the formation of axons and dendrites in non-dividing neurons [4].
In male mammalian meiosis and spermiogenesis, gene knockout mouse models have shown that KATNA1 and its paralogue KATNAL1 act co-operatively. They collectively regulate the male meiotic spindle, cytokinesis, midbody abscission, and spermatid remodelling events [2]. In neurons, studies on cultured hippocampal neurons (a form of in-vitro functional study relevant to in-vivo neural function) have demonstrated that KATNA1 promotes neurite outgrowth. Mutation of the SUMOylation site K330 in KATNA1 abolishes its microtubule-severing ability and the promotion of neurite outgrowth, indicating that SUMOylation at this site is important for its function [1]. Also, the interaction between KATNA1 and CRMP3 has been shown to enhance microtubule-severing efficiency and promote hippocampal neurite outgrowth, as genetic knockout of either protein significantly inhibits neurite outgrowth [3].
In conclusion, KATNA1 is vital for microtubule-related biological processes, especially in neural development and male reproductive processes. Gene knockout models, both in the context of meiosis and neural cell function studies, have provided key insights into its functions. Understanding KATNA1's role can potentially contribute to research in neurodegenerative diseases and male infertility [1,2,3].
References:
1. Li, Shaojin, Liang, Yaozhong, Zou, Jianyu, Zhang, Guowei, Tan, Minghui. 2022. SUMOylation of microtubule-cleaving enzyme KATNA1 promotes microtubule severing and neurite outgrowth. In The Journal of biological chemistry, 298, 102292. doi:10.1016/j.jbc.2022.102292. https://pubmed.ncbi.nlm.nih.gov/35868557/
2. Dunleavy, Jessica E M, Graffeo, Maddison, Wozniak, Kathryn, Houston, Brendan J, O'Bryan, Moira K. 2023. The katanin A-subunits KATNA1 and KATNAL1 act co-operatively in mammalian meiosis and spermiogenesis to achieve male fertility. In Development (Cambridge, England), 150, . doi:10.1242/dev.201956. https://pubmed.ncbi.nlm.nih.gov/37882691/
3. Xie, Zhiyao, Song, Wei, Meng, Zhichao, Lei, Changbin, Tan, Minghui. 2025. The interaction between KATNA1 and CRMP3 modulates microtubule dynamics and neurite outgrowth. In Biochemical and biophysical research communications, 752, 151426. doi:10.1016/j.bbrc.2025.151426. https://pubmed.ncbi.nlm.nih.gov/39938451/
4. Kaya, Yesim, Korulu, Sirin, Tunoglu, Ezgi Nurdan Yenilmez, Yildiz, Aysegul. 2023. A potential posttranscriptional regulator for p60-katanin: miR-124-3p. In Cytoskeleton (Hoboken, N.J.), 80, 437-447. doi:10.1002/cm.21769. https://pubmed.ncbi.nlm.nih.gov/37439368/
5. Cheung, Keith, Senese, Silvia, Kuang, Jiaen, Whitelegge, Julian P, Torres, Jorge Z. 2016. Proteomic Analysis of the Mammalian Katanin Family of Microtubule-severing Enzymes Defines Katanin p80 subunit B-like 1 (KATNBL1) as a Regulator of Mammalian Katanin Microtubule-severing. In Molecular & cellular proteomics : MCP, 15, 1658-69. doi:10.1074/mcp.M115.056465. https://pubmed.ncbi.nlm.nih.gov/26929214/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen