C57BL/6JCya-Ago2em1flox/Cya
Common Name:
Ago2-flox
Product ID:
S-CKO-08136
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ago2-flox
Strain ID
CKOCMP-239528-Ago2-B6J-VA
Gene Name
Product ID
S-CKO-08136
Gene Alias
1110029L17Rik; 2310051F07Rik; Eif2c2; Gerp95; Gm10365; mKIAA4215
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ago2em1flox/Cya mice (Catalog S-CKO-08136) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000044113
NCBI RefSeq
NM_153178
Target Region
Exon 6~7
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Ago2, the only member with catalytic activity in the Argonaute family, is a key component of the RNA-induced silencing complex. It contains four functional core domains (N, PAZ, MID, and PIWI from N-to C-terminal) and is involved in multiple processes. It plays a crucial role in small RNAs guided post-transcriptional gene silencing, such as mRNA degradation and translational repression. Additionally, Ago2 is implicated in gene regulation in nuclei, including chromatin remodeling, transcriptional regulation, double-strand break repair, and alternative splicing. It also participates in other cellular processes like alternative polyadenylation, translational activation, and transposon repression [3].
Deletion of YTHDF1, a m6A reader protein, leads to changes in Ago2-related functions. YTHDF1 interacts with Ago2 through its YTH domain, and their co-localization in P-body is observed. YTHDF1 promotes mRNA degradation by recruiting Ago2 and facilitating P-body formation via liquid-liquid phase separation. The deletion of YTHDF1 causes P-bodies to change from liquid to gel/solid droplets, resulting in Ago2/RNA patches and mRNA degradation delay, revealing a new aspect of mRNA post-transcriptional regulation [1]. In failing hearts, nuclear Ago2, rather than cytosolic Ago2, overexpression exacerbates cardiac dysfunction. Nuclear Ago2 activates the transcription of ANKRD1, which is involved in cardiac remodeling. Drugs like ivermectin and ANPep can prevent ANKRD1 nuclear import and improve cardiac performance [2].
In summary, Ago2 is essential for post-transcriptional gene silencing and various gene-regulatory processes. Studies using gene-knockout models, like the YTHDF1-KO cell lines and overexpression models in the heart, have revealed its role in mRNA regulation and cardiac remodeling. These findings contribute to our understanding of Ago2's function in normal biological processes and disease conditions such as heart failure and potentially provide new therapeutic targets.
References:
1. Li, Jiong, Chen, Ke, Dong, Xin, He, Chunjiang, Luo, Mengcheng. 2021. YTHDF1 promotes mRNA degradation via YTHDF1-AGO2 interaction and phase separation. In Cell proliferation, 55, e13157. doi:10.1111/cpr.13157. https://pubmed.ncbi.nlm.nih.gov/34821414/
2. Xie, Rong, Yuan, Shuai, Hu, Guo, Wang, Dao Wen, Li, Huaping. 2024. Nuclear AGO2 promotes myocardial remodeling by activating ANKRD1 transcription in failing hearts. In Molecular therapy : the journal of the American Society of Gene Therapy, 32, 1578-1594. doi:10.1016/j.ymthe.2024.03.018. https://pubmed.ncbi.nlm.nih.gov/38475992/
3. Li, Xiaojing, Wang, Xueying, Cheng, Zeneng, Zhu, Qubo. 2020. AGO2 and its partners: a silencing complex, a chromatin modulator, and new features. In Critical reviews in biochemistry and molecular biology, 55, 33-53. doi:10.1080/10409238.2020.1738331. https://pubmed.ncbi.nlm.nih.gov/32164444/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen