C57BL/6JCya-Carfem1flox/Cya
Common Name:
Carf-flox
Product ID:
S-CKO-08328
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Carf-flox
Strain ID
CKOCMP-241066-Carf-B6J-VA
Gene Name
Product ID
S-CKO-08328
Gene Alias
Als2cr8; ECBRC-FC1; ECBRC-FC2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Carfem1flox/Cya mice (Catalog S-CKO-08328) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000187978
NCBI RefSeq
NM_139150
Target Region
Exon 8
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
CARF, also known as CDKN2AIP and Collaborator of ARF, is a multi-module regulator that plays a crucial role in cell proliferation, acting as a molecular bridge between cellular senescence and carcinogenesis [1,2,5]. It stabilizes the p53-tumor suppressor protein, either in an ARF-dependent or-independent manner, and transcriptionally represses HDM2, which negatively regulates p53 through proteasomal-mediated degradation. Thus, CARF controls the p53-HDM2-p21WAF1 axis to regulate cell proliferative fates [1].
In male CARF knockout mice, fertility is significantly impaired, and they exhibit Sertoli-cell-only (SCO) syndrome phenotypes. Loss of CARF in Sertoli cells leads to decreased GDNF expression, hindering spermatogonial stem cells (SSCs) self-renewal. In undifferentiated spermatogonia, CARF loss impairs their proliferation [3]. Additionally, fatty livers from mice have low CARF expression. Silencing and overexpressing CARF result in higher and lower fat accumulation in HepG2 cells respectively, suggesting CARF dysregulation by excess fat leads to hepatic steatosis [4]. In colorectal cancer, aberrant upregulation of CARF is found in tumor tissues, promoting cancer stemness by activating the ERBB signaling pathway [6].
In conclusion, CARF has essential functions in regulating cell proliferation, spermatogenesis, and lipid metabolism. The study of CARF knockout mouse models has revealed its roles in male infertility, hepatic steatosis, and colorectal cancer, providing insights into the underlying molecular mechanisms and potential therapeutic targets for these disease conditions.
References:
1. Wadhwa, Renu, Kalra, Rajkumar Singh, Kaul, Sunil C. 2017. CARF is a multi-module regulator of cell proliferation and a molecular bridge between cellular senescence and carcinogenesis. In Mechanisms of ageing and development, 166, 64-68. doi:10.1016/j.mad.2017.07.008. https://pubmed.ncbi.nlm.nih.gov/28754531/
2. Cheung, Caroline T Y, Hasan, Md Kamrul, Widodo, Nashi, Kaul, Sunil C, Wadhwa, Renu. 2008. CARF: an emerging regulator of p53 tumor suppressor and senescence pathway. In Mechanisms of ageing and development, 130, 18-23. doi:10.1016/j.mad.2008.05.002. https://pubmed.ncbi.nlm.nih.gov/18555516/
3. Cui, Wenhao, He, Xiaoli, Zhai, Xiaohong, Shi, Qinghua, Li, Lin. 2020. CARF promotes spermatogonial self-renewal and proliferation through Wnt signaling pathway. In Cell discovery, 6, 85. doi:10.1038/s41421-020-00212-7. https://pubmed.ncbi.nlm.nih.gov/33298864/
4. Hasan, Kamrul M, Parveen, Meher, Pena, Alondra, Sinha-Hikim, Amiya P, Friedman, Theodore C. 2023. Fatty Acid Excess Dysregulates CARF to Initiate the Development of Hepatic Steatosis. In Cells, 12, . doi:10.3390/cells12071069. https://pubmed.ncbi.nlm.nih.gov/37048142/
5. Cheung, Caroline T, Kaul, Sunil C, Wadhwa, Renu. . Molecular bridging of aging and cancer: A CARF link. In Annals of the New York Academy of Sciences, 1197, 129-33. doi:10.1111/j.1749-6632.2009.05392.x. https://pubmed.ncbi.nlm.nih.gov/20536841/
6. Dong, Weiyi, Cao, Zheng, Pang, Yanmin, Feng, Teng, Tian, Hongtao. 2019. CARF, As An Oncogene, Promotes Colorectal Cancer Stemness By Activating ERBB Signaling Pathway. In OncoTargets and therapy, 12, 9041-9051. doi:10.2147/OTT.S225733. https://pubmed.ncbi.nlm.nih.gov/31802911/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen