Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Xrn1em1flox/Cya
Common Name:
Xrn1-flox
Product ID:
S-CKO-08352
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Xrn1-flox
Strain ID
CKOCMP-24127-Xrn1-B6J-VA
Gene Name
Xrn1
Product ID
S-CKO-08352
Gene Alias
Dhm2; exo; mXrn1
Background
C57BL/6JCya
NCBI ID
24127
Modification
Conditional knockout
Chromosome
9
Phenotype
MGI:891964
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Xrn1em1flox/Cya mice (Catalog S-CKO-08352) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034981
NCBI RefSeq
NM_011916
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Xrn1, an exoribonuclease, plays a crucial role in RNA decay, executing the last step of mRNA degradation in the cytoplasm along the 5'-3' direction [3,4,5,9]. It is involved in multiple pathways such as dsRNA sensing, mRNA decapping and decay, and is associated with important biological processes like reproduction, metabolism, and immune response [1,2,3,5,6,7,8]. Genetic models, especially knockout models, are valuable for studying its functions.

In interferon-activated cancer cells, Xrn1 deletion causes PKR-dependent cell lethality, highlighting its role as a negative regulator of the PKR pathway [1,2]. In Macrobrachium nipponense, knockdown of Mn-XRN1 accelerates ovarian development, indicating its inhibitory effect on ovarian reproduction [5]. In forebrain-specific Xrn1 conditional knockout (Xrn1-cKO) mice, obesity, leptin resistance, hyperglycemia, hyperphagia, and decreased energy expenditure occur, suggesting that XRN1 in the hypothalamus is critical for metabolic homeostasis [8]. Targeting XRN1 in cancer cells can also potentiate the efficacy of cancer immunotherapy as its depletion activates IFN signaling and the viral defense pathway [7].

In conclusion, Xrn1 is essential for RNA decay and is involved in diverse biological processes. Gene knockout and conditional knockout mouse models have revealed its significance in cancer, reproduction, and metabolic diseases. These findings offer potential therapeutic targets for related diseases and enhance our understanding of the underlying molecular mechanisms.

References:
1. Zou, Tao, Zhou, Meng, Gupta, Akansha, Cherniack, Andrew D, Meyerson, Matthew. 2024. XRN1 deletion induces PKR-dependent cell lethality in interferon-activated cancer cells. In Cell reports, 43, 113600. doi:10.1016/j.celrep.2023.113600. https://pubmed.ncbi.nlm.nih.gov/38261514/
2. Zou, Tao, Zhou, Meng, Gupta, Akansha, Cherniack, Andrew D, Meyerson, Matthew. 2023. XRN1 deletion induces PKR-dependent cell lethality in interferon-activated cancer cells. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.08.01.551488. https://pubmed.ncbi.nlm.nih.gov/37577567/
3. Brothers, William R, Ali, Farah, Kajjo, Sam, Fabian, Marc R. 2023. The EDC4-XRN1 interaction controls P-body dynamics to link mRNA decapping with decay. In The EMBO journal, 42, e113933. doi:10.15252/embj.2023113933. https://pubmed.ncbi.nlm.nih.gov/37621215/
4. Sharma, Sunny, Yang, Jun, Grudzien-Nogalska, Ewa, Kwan, Kelvin Y, Kiledjian, Megerditch. 2022. Xrn1 is a deNADding enzyme modulating mitochondrial NAD-capped RNA. In Nature communications, 13, 889. doi:10.1038/s41467-022-28555-7. https://pubmed.ncbi.nlm.nih.gov/35173156/
5. Chen, Tianyong, Yuan, Huwei, Qiao, Hui, Fu, Hongtuo, Jin, Shubo. 2023. Mn-XRN1 Has an Inhibitory Effect on Ovarian Reproduction in Macrobrachium nipponense. In Genes, 14, . doi:10.3390/genes14071454. https://pubmed.ncbi.nlm.nih.gov/37510358/
6. Hosseini, Amir, Lindholm, Håvard T, Chen, Raymond, Greenbaum, Benjamin, De Carvalho, Daniel D. 2024. Retroelement decay by the exonuclease XRN1 is a viral mimicry dependency in cancer. In Cell reports, 43, 113684. doi:10.1016/j.celrep.2024.113684. https://pubmed.ncbi.nlm.nih.gov/38261511/
7. Ran, Xue-Bin, Ding, Ling-Wen, Sun, Qiao-Yang, Jha, Sudhakar, Koeffler, H Phillip. . Targeting RNA Exonuclease XRN1 Potentiates Efficacy of Cancer Immunotherapy. In Cancer research, 83, 922-938. doi:10.1158/0008-5472.CAN-21-3052. https://pubmed.ncbi.nlm.nih.gov/36638333/
8. Takaoka, Shohei, Yanagiya, Akiko, Mohamed, Haytham Mohamed Aly, Stoney, Patrick, Yamamoto, Tadashi. 2021. Neuronal XRN1 is required for maintenance of whole-body metabolic homeostasis. In iScience, 24, 103151. doi:10.1016/j.isci.2021.103151. https://pubmed.ncbi.nlm.nih.gov/34646989/
9. Grousl, Tomas, Vomastek, Tomas. . Xrn1 Exoribonuclease-An Intrinsic Marker of Yeast Population Growth. In Frontiers in bioscience (Elite edition), 16, 1. doi:10.31083/j.fbe1601001. https://pubmed.ncbi.nlm.nih.gov/38538525/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest