C57BL/6JCya-Xrn1em1flox/Cya
Common Name:
Xrn1-flox
Product ID:
S-CKO-08352
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Xrn1-flox
Strain ID
CKOCMP-24127-Xrn1-B6J-VA
Gene Name
Product ID
S-CKO-08352
Gene Alias
Dhm2; exo; mXrn1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Xrn1em1flox/Cya mice (Catalog S-CKO-08352) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034981
NCBI RefSeq
NM_011916
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Xrn1, an exoribonuclease, plays a crucial role in RNA decay, executing the last step of mRNA degradation in the cytoplasm along the 5'-3' direction [3,4,5,9]. It is involved in multiple pathways such as dsRNA sensing, mRNA decapping and decay, and is associated with important biological processes like reproduction, metabolism, and immune response [1,2,3,5,6,7,8]. Genetic models, especially knockout models, are valuable for studying its functions.
In interferon-activated cancer cells, Xrn1 deletion causes PKR-dependent cell lethality, highlighting its role as a negative regulator of the PKR pathway [1,2]. In Macrobrachium nipponense, knockdown of Mn-XRN1 accelerates ovarian development, indicating its inhibitory effect on ovarian reproduction [5]. In forebrain-specific Xrn1 conditional knockout (Xrn1-cKO) mice, obesity, leptin resistance, hyperglycemia, hyperphagia, and decreased energy expenditure occur, suggesting that XRN1 in the hypothalamus is critical for metabolic homeostasis [8]. Targeting XRN1 in cancer cells can also potentiate the efficacy of cancer immunotherapy as its depletion activates IFN signaling and the viral defense pathway [7].
In conclusion, Xrn1 is essential for RNA decay and is involved in diverse biological processes. Gene knockout and conditional knockout mouse models have revealed its significance in cancer, reproduction, and metabolic diseases. These findings offer potential therapeutic targets for related diseases and enhance our understanding of the underlying molecular mechanisms.
References:
1. Zou, Tao, Zhou, Meng, Gupta, Akansha, Cherniack, Andrew D, Meyerson, Matthew. 2024. XRN1 deletion induces PKR-dependent cell lethality in interferon-activated cancer cells. In Cell reports, 43, 113600. doi:10.1016/j.celrep.2023.113600. https://pubmed.ncbi.nlm.nih.gov/38261514/
2. Zou, Tao, Zhou, Meng, Gupta, Akansha, Cherniack, Andrew D, Meyerson, Matthew. 2023. XRN1 deletion induces PKR-dependent cell lethality in interferon-activated cancer cells. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.08.01.551488. https://pubmed.ncbi.nlm.nih.gov/37577567/
3. Brothers, William R, Ali, Farah, Kajjo, Sam, Fabian, Marc R. 2023. The EDC4-XRN1 interaction controls P-body dynamics to link mRNA decapping with decay. In The EMBO journal, 42, e113933. doi:10.15252/embj.2023113933. https://pubmed.ncbi.nlm.nih.gov/37621215/
4. Sharma, Sunny, Yang, Jun, Grudzien-Nogalska, Ewa, Kwan, Kelvin Y, Kiledjian, Megerditch. 2022. Xrn1 is a deNADding enzyme modulating mitochondrial NAD-capped RNA. In Nature communications, 13, 889. doi:10.1038/s41467-022-28555-7. https://pubmed.ncbi.nlm.nih.gov/35173156/
5. Chen, Tianyong, Yuan, Huwei, Qiao, Hui, Fu, Hongtuo, Jin, Shubo. 2023. Mn-XRN1 Has an Inhibitory Effect on Ovarian Reproduction in Macrobrachium nipponense. In Genes, 14, . doi:10.3390/genes14071454. https://pubmed.ncbi.nlm.nih.gov/37510358/
6. Hosseini, Amir, Lindholm, Håvard T, Chen, Raymond, Greenbaum, Benjamin, De Carvalho, Daniel D. 2024. Retroelement decay by the exonuclease XRN1 is a viral mimicry dependency in cancer. In Cell reports, 43, 113684. doi:10.1016/j.celrep.2024.113684. https://pubmed.ncbi.nlm.nih.gov/38261511/
7. Ran, Xue-Bin, Ding, Ling-Wen, Sun, Qiao-Yang, Jha, Sudhakar, Koeffler, H Phillip. . Targeting RNA Exonuclease XRN1 Potentiates Efficacy of Cancer Immunotherapy. In Cancer research, 83, 922-938. doi:10.1158/0008-5472.CAN-21-3052. https://pubmed.ncbi.nlm.nih.gov/36638333/
8. Takaoka, Shohei, Yanagiya, Akiko, Mohamed, Haytham Mohamed Aly, Stoney, Patrick, Yamamoto, Tadashi. 2021. Neuronal XRN1 is required for maintenance of whole-body metabolic homeostasis. In iScience, 24, 103151. doi:10.1016/j.isci.2021.103151. https://pubmed.ncbi.nlm.nih.gov/34646989/
9. Grousl, Tomas, Vomastek, Tomas. . Xrn1 Exoribonuclease-An Intrinsic Marker of Yeast Population Growth. In Frontiers in bioscience (Elite edition), 16, 1. doi:10.31083/j.fbe1601001. https://pubmed.ncbi.nlm.nih.gov/38538525/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen