C57BL/6JCya-Lrrtm4em1flox/Cya
Common Name:
Lrrtm4-flox
Product ID:
S-CKO-08519
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Lrrtm4-flox
Strain ID
CKOCMP-243499-Lrrtm4-B6J-VA
Gene Name
Product ID
S-CKO-08519
Gene Alias
7530419J18Rik; A230052N11
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lrrtm4em1flox/Cya mice (Catalog S-CKO-08519) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000136421
NCBI RefSeq
NM_001282102
Target Region
Exon 2
Size of Effective Region
~4.6 kb
Detailed Document
Overview of Gene Research
Lrrtm4, leucine rich repeat transmembrane neuronal 4, is a synaptic adhesion molecule crucial for synapse formation and signaling [1,2,3,4]. It is involved in various synapse-related pathways, and its proper function is essential for normal neuronal communication. Genetic models, such as KO mouse models, are valuable for studying its functions [2,3].
In the retina, knockout of Lrrtm4 in rod bipolar cells (RBCs) reduces RBC axonal GABAA and GABAC receptor clustering, disrupts presynaptic inhibition onto RBC terminals, and perturbs the stereotyped output synapse arrangement at RBC terminals [2]. In the hippocampus, Lrrtm4(-/-) dentate gyrus granule cells exhibit reductions in excitatory synapse density and function, as well as impaired activity-regulated AMPA receptor trafficking [3].
In conclusion, Lrrtm4 plays a key role in synapse organization and function, especially in retinal and hippocampal neurons. Studies using KO mouse models have revealed its significance in regulating GABAergic synapses in the retina and excitatory synapses in the hippocampus, providing insights into potential mechanisms underlying related neurological disorders [2,3].
References：
1. Agosto, Melina A, Wensel, Theodore G. 2020. LRRTM4 is a member of the transsynaptic complex between rod photoreceptors and bipolar cells. In The Journal of comparative neurology, 529, 221-233. doi:10.1002/cne.24944. https://pubmed.ncbi.nlm.nih.gov/32390181/
2. Sinha, Raunak, Siddiqui, Tabrez J, Padmanabhan, Nirmala, Wong, Rachel O, Hoon, Mrinalini. 2020. LRRTM4: A Novel Regulator of Presynaptic Inhibition and Ribbon Synapse Arrangements of Retinal Bipolar Cells. In Neuron, 105, 1007-1017.e5. doi:10.1016/j.neuron.2019.12.028. https://pubmed.ncbi.nlm.nih.gov/31974009/
3. Siddiqui, Tabrez J, Tari, Parisa Karimi, Connor, Steven A, Brose, Nils, Craig, Ann Marie. 2013. An LRRTM4-HSPG complex mediates excitatory synapse development on dentate gyrus granule cells. In Neuron, 79, 680-95. doi:10.1016/j.neuron.2013.06.029. https://pubmed.ncbi.nlm.nih.gov/23911104/
4. de Wit, Joris, O'Sullivan, Matthew L, Savas, Jeffrey N, Yates, John R, Ghosh, Anirvan. 2013. Unbiased discovery of glypican as a receptor for LRRTM4 in regulating excitatory synapse development. In Neuron, 79, 696-711. doi:10.1016/j.neuron.2013.06.049. https://pubmed.ncbi.nlm.nih.gov/23911103/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen