C57BL/6JCya-Zfp128em1flox/Cya
Common Name:
Zfp128-flox
Product ID:
S-CKO-08543
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Zfp128-flox
Strain ID
CKOCMP-243833-Zfp128-B6J-VA
Gene Name
Product ID
S-CKO-08543
Gene Alias
9630016P15Rik; Znf8; mZnf8
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp128em1flox/Cya mice (Catalog S-CKO-08543) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000144578
NCBI RefSeq
NM_153802
Target Region
Exon 2~3
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Zfp128, also known as mZnf8, is a mouse Krüppel-type zinc finger protein. It functions as a nuclear transcriptional repressor and is involved in negatively regulating the TGF-β/BMP signaling pathway. This pathway is crucial for various biological processes, and Zfp128's role in it underscores its biological importance. Genetic models, such as mouse models, can be valuable for further exploring its functions.
Functional analysis using in vitro and in vivo approaches, including RNA interference to silence endogenous mZnf8 expression, demonstrated its role in the TGF-β/BMP signaling pathway. Silencing mZnf8 led to a significant increase in the expression of one BMP reporter, suggesting its negative regulatory role in vivo [1].
In conclusion, Zfp128 (mZnf8) is a key negative regulator of the TGF-β/BMP signaling pathway. The use of in vivo models, like the RNA interference-based silencing in this study, has helped to uncover its role in this important biological pathway, potentially providing insights relevant to diseases related to dysregulated TGF-β/BMP signaling.
References：
1. Jiao, Kai, Zhou, Yingna, Hogan, Brigid L M. . Identification of mZnf8, a mouse Krüppel-like transcriptional repressor, as a novel nuclear interaction partner of Smad1. In Molecular and cellular biology, 22, 7633-44. doi:. https://pubmed.ncbi.nlm.nih.gov/12370310/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen