C57BL/6JCya-Zfp536em1flox/Cya
Common Name:
Zfp536-flox
Product ID:
S-CKO-08560
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Zfp536-flox
Strain ID
CKOCMP-243937-Zfp536-B6J-VA
Gene Name
Product ID
S-CKO-08560
Gene Alias
9630010P11Rik; Znf536; mKIAA0390
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp536em1flox/Cya mice (Catalog S-CKO-08560) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000056338
NCBI RefSeq
NM_172385
Target Region
Exon 4
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Zfp536, a gene with currently no widely-known common aliases, is involved in multiple biological processes. It has been implicated in cell lineage differentiation, such as in dermal fibroblast development where it is a putative key driver gene for the papillary fibroblast trajectory [1]. It also seems to play a role in the development of the cerebral cortex, with a sense lncRNA SenZfp536 cis-regulating Zfp536 and affecting the proliferation of cortical neural progenitor cells [2]. In tooth initiation, Zfp536 is among the transcription factors found near the Sox2:Tfap2a/Tfap2b interface in the mandibular epithelium, suggesting its involvement in transcriptional regulatory networks controlling tooth development [3]. Additionally, it has been identified as a candidate gene for obesity through whole-genome sequencing of mouse lines divergently selected for fatness and leanness [4].
In the generation of oligodendroglial cells, forced expression of Zfp536 along with Sox10 and Olig2 can reprogram mouse and rat fibroblasts into induced oligodendrocyte precursor cells (iOPCs) with morphologies and gene expression signatures resembling primary OPCs [6]. Different combinations of transcription factors show differential efficiency in reprogramming iOPCs from mouse and rat fibroblasts, with the combination of Olig2, Sox10, and Zfp536 being more effective in rat fibroblasts [5]. Moreover, drugs can enhance the myelinating capacities of iOPCs generated using Zfp536-based conversion [7]. In the context of lens development, shRNA-mediated knockdown of Pax6, a key regulator of lens formation, leads to down-regulation of Zfp536, accompanied by changes in histone H3K4 methylation at enhancers and promoters [8].
In summary, Zfp536 is involved in diverse biological processes including cell lineage differentiation, neural progenitor cell proliferation, tooth development, and potentially obesity. Its role in generating oligodendroglial cells through direct lineage conversion has implications for disease modeling and regenerative medicine. The study of Zfp536 using gene-knockdown and other model-based research methods helps in understanding these biological processes and provides insights into potential therapeutic strategies related to demyelinating diseases and other conditions [1-4, 6-10].
References:
1. Lee, Hanjae, Kim, So Young, Kwon, Nak-Jung, Kwon, Ohsang, Kim, Jong-Il. 2023. Single-Cell and Spatial Transcriptome Analysis of Dermal Fibroblast Development in Perinatal Mouse Skin: Dynamic Lineage Differentiation and Key Driver Genes. In The Journal of investigative dermatology, 144, 1238-1250.e11. doi:10.1016/j.jid.2023.11.008. https://pubmed.ncbi.nlm.nih.gov/38072389/
2. Tian, Kuan, Wang, Andi, Wang, Junbao, Liu, Ying, Zhou, Yan. 2020. Transcriptome Analysis Identifies SenZfp536, a Sense LncRNA that Suppresses Self-renewal of Cortical Neural Progenitors. In Neuroscience bulletin, 37, 183-200. doi:10.1007/s12264-020-00607-2. https://pubmed.ncbi.nlm.nih.gov/33196962/
3. Shao, Fan, Phan, An-Vi, Yu, Wenjie, Van Otterloo, Eric, Cao, Huojun. 2024. Transcriptional programs of Pitx2 and Tfap2a/Tfap2b controlling lineage specification of mandibular epithelium during tooth initiation. In PLoS genetics, 20, e1011364. doi:10.1371/journal.pgen.1011364. https://pubmed.ncbi.nlm.nih.gov/39052671/
4. Šimon, Martin, Mikec, Špela, Atanur, Santosh S, Horvat, Simon, Kunej, Tanja. 2024. Whole genome sequencing of mouse lines divergently selected for fatness (FLI) and leanness (FHI) revealed several genetic variants as candidates for novel obesity genes. In Genes & genomics, 46, 557-575. doi:10.1007/s13258-024-01507-9. https://pubmed.ncbi.nlm.nih.gov/38483771/
5. Lee, Eun-Hye, Park, Chang-Hwan. . Comparison of Reprogramming Methods for Generation of Induced-Oligodendrocyte Precursor Cells. In Biomolecules & therapeutics, 25, 362-366. doi:10.4062/biomolther.2017.066. https://pubmed.ncbi.nlm.nih.gov/28605832/
6. Yang, Nan, Zuchero, J Bradley, Ahlenius, Henrik, Barres, Ben A, Wernig, Marius. 2013. Generation of oligodendroglial cells by direct lineage conversion. In Nature biotechnology, 31, 434-9. doi:10.1038/nbt.2564. https://pubmed.ncbi.nlm.nih.gov/23584610/
7. Pascual-Guerra, J, Torres-Rico, M, Marín-Rodríguez, B, Rodríguez-Navarro, J A, Paíno, C L. 2025. Repurposed Drugs to Enhance the Therapeutic Potential of Oligodendrocyte Precursor Cells Derived from Adult Rat Adipose Tissue. In Cells, 14, . doi:10.3390/cells14070533. https://pubmed.ncbi.nlm.nih.gov/40214487/
8. Sun, Jian, Zhao, Yilin, McGreal, Rebecca, Zheng, Deyou, Cvekl, Ales. 2016. Pax6 associates with H3K4-specific histone methyltransferases Mll1, Mll2, and Set1a and regulates H3K4 methylation at promoters and enhancers. In Epigenetics & chromatin, 9, 37. doi:10.1186/s13072-016-0087-z. https://pubmed.ncbi.nlm.nih.gov/27617035/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen