C57BL/6JCya-Mrgprb2em1flox/Cya
Common Name:
Mrgprb2-flox
Product ID:
S-CKO-08565
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mrgprb2-flox
Strain ID
CKOCMP-243979-Mrgprb2-B6J-VA
Gene Name
Product ID
S-CKO-08565
Gene Alias
4833406I20Rik; Mgrg14
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mrgprb2em1flox/Cya mice (Catalog S-CKO-08565) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000052730
NCBI RefSeq
NM_175531
Target Region
Exon 2
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Mrgprb2, the mouse ortholog of human MRGPRX2, is a mast-cell-specific G-protein-coupled receptor. It plays a crucial role in non-IgE-mediated mast cell activation, participating in various physiological and pathological processes such as neuro-immune regulation, inflammation, and pain response. Signaling through Mrgprb2 activates multiple pathways, and its activation can lead to the release of pro-inflammatory cytokines, chemokines, and enzymes like tryptase, which are involved in the development of inflammation [1,2,3,4,5,6,7,8,9,10].
In atopic dermatitis (AD) mouse models, Mrgprb2-conditional knockout (Mrgprb2-/-) mice showed milder phenotypes and less inflammatory infiltration compared to wild-type mice. Tryptase released by MRGPRX2/MRGPRB2 activation contributed to type 2 cytokine release and AD-related inflammation [1]. In Modic changes models, Mrgprb2 knockout inhibited mast cell activation, reducing the degree of Modic changes, and Mrgprb2-activated mast cells regulated immune niches by influencing macrophage polarization [3]. In acute colitis models, Mrgprb2-/-mice had impaired DSS-induced neutrophil influx and less severe colitis progression, indicating its role in colitis pathophysiology [5]. Alcohol-withdrawal-induced headache behaviors were absent in Mrgprb2-deficient mice, suggesting its role in this condition [7]. Mrgprb2-deficiency also decreased itch in allergic contact dermatitis models [8].
In conclusion, Mrgprb2 is a key mediator in mast-cell-related immune and inflammatory responses. Gene knockout mouse models have significantly advanced our understanding of its role in diseases such as atopic dermatitis, Modic changes, acute colitis, alcohol-withdrawal-associated headache, and allergic contact dermatitis. These studies provide potential therapeutic targets for treating these conditions by modulating Mrgprb2-mediated pathways.
References:
1. Jia, Tao, Che, Delu, Zheng, Yi, An, Jingang, Geng, Songmei. 2023. Mast Cells Initiate Type 2 Inflammation through Tryptase Released by MRGPRX2/MRGPRB2 Activation in Atopic Dermatitis. In The Journal of investigative dermatology, 144, 53-62.e2. doi:10.1016/j.jid.2023.06.201. https://pubmed.ncbi.nlm.nih.gov/37482287/
2. Bawazir, Maram, Amponnawarat, Aetas, Hui, Yvonne, Oskeritzian, Carole A, Ali, Hydar. 2022. Inhibition of MRGPRX2 but not FcεRI or MrgprB2-mediated mast cell degranulation by a small molecule inverse receptor agonist. In Frontiers in immunology, 13, 1033794. doi:10.3389/fimmu.2022.1033794. https://pubmed.ncbi.nlm.nih.gov/36275683/
3. Ji, Zhongyin, Li, Jie, Tao, Siyue, Liu, Junhui, Zhao, Fengdong. 2024. Mrgprb2-mediated mast cell activation exacerbates Modic changes by regulating immune niches. In Experimental & molecular medicine, 56, 1178-1192. doi:10.1038/s12276-024-01230-1. https://pubmed.ncbi.nlm.nih.gov/38689089/
4. Thapaliya, Monica, Ali, Hydar. 2023. GRK2 differentially regulates FcεRI and MRGPRB2-mediated responses in mast cells. In Frontiers in immunology, 14, 1155777. doi:10.3389/fimmu.2023.1155777. https://pubmed.ncbi.nlm.nih.gov/37063868/
5. Van Remoortel, Samuel, Lambeets, Lana, De Winter, Benedicte, Martinez, Sales Ibiza, Timmermans, Jean-Pierre. 2024. Mrgprb2-dependent Mast Cell Activation Plays a Crucial Role in Acute Colitis. In Cellular and molecular gastroenterology and hepatology, 18, 101391. doi:10.1016/j.jcmgh.2024.101391. https://pubmed.ncbi.nlm.nih.gov/39179175/
6. Green, Dustin P, Limjunyawong, Nathachit, Gour, Naina, Pundir, Priyanka, Dong, Xinzhong. 2019. A Mast-Cell-Specific Receptor Mediates Neurogenic Inflammation and Pain. In Neuron, 101, 412-420.e3. doi:10.1016/j.neuron.2019.01.012. https://pubmed.ncbi.nlm.nih.gov/30686732/
7. Son, Hyeonwi, Zhang, Yan, Shannonhouse, John, Gomez, Ruben, Kim, Yu Shin. 2023. Mast-cell-specific receptor mediates alcohol-withdrawal-associated headache in male mice. In Neuron, 112, 113-123.e4. doi:10.1016/j.neuron.2023.09.039. https://pubmed.ncbi.nlm.nih.gov/37909038/
8. Meixiong, James, Anderson, Michael, Limjunyawong, Nathachit, Kim, Brian S, Dong, Xinzhong. 2019. Activation of Mast-Cell-Expressed Mas-Related G-Protein-Coupled Receptors Drives Non-histaminergic Itch. In Immunity, 50, 1163-1171.e5. doi:10.1016/j.immuni.2019.03.013. https://pubmed.ncbi.nlm.nih.gov/31027996/
9. Duraisamy, Karthi, Kumar, Mukesh, Nawabjan, Abdullah, Leprince, Jérôme, Chow, Billy K C. 2024. MRGPRB2/X2 and the analogous effects of its agonist and antagonist in DSS-induced colitis in mice. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 174, 116471. doi:10.1016/j.biopha.2024.116471. https://pubmed.ncbi.nlm.nih.gov/38547764/
10. Wang, Jue, Zhang, Yongjing, Che, Delu, Qin, Qiaohong, Wang, Nan. 2020. Baicalin induces Mrgprb2-dependent pseudo-allergy in mice. In Immunology letters, 226, 55-61. doi:10.1016/j.imlet.2020.07.006. https://pubmed.ncbi.nlm.nih.gov/32707128/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen