C57BL/6JCya-Fsd2em1flox/Cya
Common Name:
Fsd2-flox
Product ID:
S-CKO-08572
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fsd2-flox
Strain ID
CKOCMP-244091-Fsd2-B6J-VA
Gene Name
Product ID
S-CKO-08572
Gene Alias
9830160G03Rik; Spryd1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fsd2em1flox/Cya mice (Catalog S-CKO-08572) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000042318
NCBI RefSeq
NM_172904
Target Region
Exon 4~5
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Fsd2, or Fibronectin Type III and SPRY Domain Containing 2, is associated with multiple biological functions. In plants like Arabidopsis thaliana, it is a chloroplast-localized iron superoxide dismutase, functioning in detoxifying superoxide, protecting chloroplast nucleoids from oxidative stress, and being essential for early chloroplast development [1,2,6,7]. In pigs, it's considered a candidate gene for pork quality, with its haplotypes related to intramuscular fat content, moisture percentage, and meat color [4]. In mammals, it's part of the myospryn complex in cardiac and skeletal muscle, interacting with proteins like Speg and Cmya5, and playing a role in stabilizing excitation-contraction coupling protein complexes [3,5,8].
In Arabidopsis, loss-of-function mutants of Fsd2 exhibit increased superoxide production, reduced chlorophyll levels, lower photosynthetic efficiency, and impaired growth [1]. In pap9/fsd2 mutants, under heat stress, there are declines in chlorophyll content and photosynthesis level, and abnormal regulation of photosynthesis-related genes [2]. In mice, Speg deficiency leads to a decrease in Fsd2 protein levels, accompanied by defective triad formation, abnormal excitation-contraction coupling, and calcium mishandling in skeletal muscles [5].
In summary, Fsd2 plays crucial roles in plant photosynthesis-related stress responses and in maintaining the stability of muscle-related protein complexes in animals. The study of Fsd2 through gene-knockout models in plants and mice has provided insights into its functions in these biological processes, which may have implications for understanding plant stress-tolerance mechanisms and muscle-related diseases.
References:
1. Gallie, Daniel R, Chen, Zhong. 2019. Chloroplast-localized iron superoxide dismutases FSD2 and FSD3 are functionally distinct in Arabidopsis. In PloS one, 14, e0220078. doi:10.1371/journal.pone.0220078. https://pubmed.ncbi.nlm.nih.gov/31329637/
2. Bychkov, Ivan A, Andreeva, Aleksandra A, Kudryakova, Natalia V, Pojidaeva, Elena S, Kusnetsov, Victor V. 2022. The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. In Plant science : an international journal of experimental plant biology, 322, 111359. doi:10.1016/j.plantsci.2022.111359. https://pubmed.ncbi.nlm.nih.gov/35738478/
3. Lee, Chang Seok, Jung, Sung Yun, Yee, Rachel Sue Zhen, Rodney, George G, Hamilton, Susan L. 2023. Speg interactions that regulate the stability of excitation-contraction coupling protein complexes in triads and dyads. In Communications biology, 6, 942. doi:10.1038/s42003-023-05330-y. https://pubmed.ncbi.nlm.nih.gov/37709832/
4. Lim, Kyu-Sang, Lee, Kyung-Tai, Lee, Si-Woo, Hong, Ki-Chang, Kim, Tae-Hun. 2016. Genomic structure, expression and association study of the porcine FSD2. In Molecular biology reports, 43, 1011-8. doi:10.1007/s11033-016-4029-4. https://pubmed.ncbi.nlm.nih.gov/27350214/
5. Li, Qifei, Lin, Jasmine, Luo, Shiyu, Perrella, Mark A, Agrawal, Pankaj B. 2024. Integrated multi-omics approach reveals the role of striated muscle preferentially expressed protein kinase in skeletal muscle including its relationship with myospryn complex. In Journal of cachexia, sarcopenia and muscle, 15, 1003-1015. doi:10.1002/jcsm.13470. https://pubmed.ncbi.nlm.nih.gov/38725372/
6. Garcia-Molina, Antoni, Altmann, Melina, Alkofer, Angela, Dangl, Jeffery L, Falter-Braun, Pascal. . LSU network hubs integrate abiotic and biotic stress responses via interaction with the superoxide dismutase FSD2. In Journal of experimental botany, 68, 1185-1197. doi:10.1093/jxb/erw498. https://pubmed.ncbi.nlm.nih.gov/28207043/
7. Myouga, Fumiyoshi, Hosoda, Chieko, Umezawa, Taishi, Ikeuchi, Masahiko, Shinozaki, Kazuo. 2008. A heterocomplex of iron superoxide dismutases defends chloroplast nucleoids against oxidative stress and is essential for chloroplast development in Arabidopsis. In The Plant cell, 20, 3148-62. doi:10.1105/tpc.108.061341. https://pubmed.ncbi.nlm.nih.gov/18996978/
8. Benson, Matthew A, Tinsley, Caroline L, Waite, Adrian J, Martin-Rendon, Enca, Blake, Derek J. 2017. Ryanodine receptors are part of the myospryn complex in cardiac muscle. In Scientific reports, 7, 6312. doi:10.1038/s41598-017-06395-6. https://pubmed.ncbi.nlm.nih.gov/28740084/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen