C57BL/6JCya-Kat6aem1flox/Cya
Common Name:
Kat6a-flox
Product ID:
S-CKO-08595
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Kat6a-flox
Strain ID
CKOCMP-244349-Kat6a-B6J-VA
Gene Name
Product ID
S-CKO-08595
Gene Alias
1500036M03; 9930021N24Rik; MOZ; Myst3; Zfp220
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kat6aem1flox/Cya mice (Catalog S-CKO-08595) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000044331
NCBI RefSeq
NM_001081149
Target Region
Exon 4
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
KAT6A, also known as MOZ (monocytic leukemia zinc finger protein) and MYST3, is a histone lysine acetyltransferase belonging to the MYST family. It acetylates histone H3K23 and plays crucial roles in multiple biological processes, including embryonic development, hematopoiesis, and neural stem cell maintenance. It is involved in pathways such as estrogen signaling, cell cycle, Myc, and stem cell pathways [1,2,6]. Genetic models, like KO/CKO mouse models, are valuable for studying its functions.
In cancer research, loss-of-function studies have shown potential therapeutic implications. In acute myeloid leukemia (AML), inhibition of KAT6A in vitro and in vivo led to strong anti-AML phenotypes, suggesting it could be a therapeutic target for AML treatment [3]. In triple-negative breast cancer (TNBC), inhibiting KAT6A in combination with anti-PD-L1 therapy attenuated metastasis in TNBC xenograft-bearing animals [4]. Inhibitors of KAT6A/B, such as WM-8014 and WM-1119, induced cell cycle exit and cellular senescence without DNA damage, and WM-1119 arrested lymphoma progression in mice [5]. High levels of KAT6A promote PARPi resistance in ovarian cancer, and inhibiting its liquid-liquid phase separation restores PARPi sensitivity in vivo and in vitro [7].
In conclusion, KAT6A is essential for various biological processes and is implicated in multiple disease conditions, especially cancer. Studies using KO/CKO mouse models have significantly contributed to understanding its role in cancer, highlighting its potential as a therapeutic target for cancer treatment.
References:
1. Sharma, Shikhar, Chung, Chi-Yeh, Uryu, Sean, Visvader, Jane E, Paul, Thomas A. 2023. Discovery of a highly potent, selective, orally bioavailable inhibitor of KAT6A/B histone acetyltransferases with efficacy against KAT6A-high ER+ breast cancer. In Cell chemical biology, 30, 1191-1210.e20. doi:10.1016/j.chembiol.2023.07.005. https://pubmed.ncbi.nlm.nih.gov/37557181/
2. Zu, Gaoyu, Liu, Ying, Cao, Jingli, Zhang, Hang, You, Linya. 2022. BRPF1-KAT6A/KAT6B Complex: Molecular Structure, Biological Function and Human Disease. In Cancers, 14, . doi:10.3390/cancers14174068. https://pubmed.ncbi.nlm.nih.gov/36077605/
3. Yan, Fangxue, Li, Jinyang, Milosevic, Jelena, Sykes, David B, Blanco, M Andrés. . KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs. In Cancer discovery, 12, 792-811. doi:10.1158/2159-8290.CD-20-1459. https://pubmed.ncbi.nlm.nih.gov/34853079/
4. Yu, Bo, Luo, Fei, Sun, Bowen, Li, Yanxin, Feng, Haizhong. 2021. KAT6A Acetylation of SMAD3 Regulates Myeloid-Derived Suppressor Cell Recruitment, Metastasis, and Immunotherapy in Triple-Negative Breast Cancer. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 8, e2100014. doi:10.1002/advs.202100014. https://pubmed.ncbi.nlm.nih.gov/34392614/
5. Baell, Jonathan B, Leaver, David J, Hermans, Stefan J, Voss, Anne K, Thomas, Tim. 2018. Inhibitors of histone acetyltransferases KAT6A/B induce senescence and arrest tumour growth. In Nature, 560, 253-257. doi:10.1038/s41586-018-0387-5. https://pubmed.ncbi.nlm.nih.gov/30069049/
6. Wiesel-Motiuk, Naama, Assaraf, Yehuda G. 2020. The key roles of the lysine acetyltransferases KAT6A and KAT6B in physiology and pathology. In Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 53, 100729. doi:10.1016/j.drup.2020.100729. https://pubmed.ncbi.nlm.nih.gov/33130515/
7. Zhan, Zhiyan, Zhang, Jiarong, Liang, Huisheng, Hong, Li, Liu, Wenxue. 2024. KAT6A Condensates Impair PARP1 Trapping of PARP Inhibitors in Ovarian Cancer. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2400140. doi:10.1002/advs.202400140. https://pubmed.ncbi.nlm.nih.gov/38973255/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen